Alleviating damage of photosystem and oxidative stress from chilling stress with exogenous zeaxanthin in pepper (Capsicum annuum L.) seedlings

Tang, Chaonan; Xie, Jianming; Lv, Jian; Li, Jing; Zhang, Jing; Wang, Cheng; Liang, Guoping

doi:10.1016/j.plaphy.2021.03.010

Cited by 49 publications

(45 citation statements)

References 57 publications

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“…10 mM trehalose increased the maximum electron transfer rate (rETRmax), light energy utilization efficiency (α) and hard light resistance (Ik) of tomato plants under salt stress. Tang et al (2021) also reached a similar conclusion in her study of zeaxanthin in alleviating chilling stress. Trehalose can relieve photoinhibition, which in turn will lead to positive effects on plant growth under saline conditions.…”

Section: Discussionsupporting

confidence: 58%

“…The contents of hydrogen peroxide (H 2 O 2 ) and superoxide anion (O 2 − ) were measured by the H 2 O 2 and O 2 − assay kit (Comin Botechnology, Suzhou, China). H 2 O 2 and O 2 − were visually detected in the fourth leaves according to the method of Tang et al (2021) . The activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were determined in tomato leaves using the SOD, POD, and CAT assay kit (Comin Botechnology, Suzhou, China).…”

Section: Methodsmentioning

confidence: 99%

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Trehalose Alleviated Salt Stress in Tomato by Regulating ROS Metabolism, Photosynthesis, Osmolyte Synthesis, and Trehalose Metabolic Pathways

Yang

Yao

et al. 2022

Front. Plant Sci.

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Trehalose plays a critical role in plant response to salinity but the involved regulatory mechanisms remain obscure. Here, this study explored the mechanism of exogenous trehalose-induced salt tolerance in tomato plants by the hydroponic test method. Our results indicated that 10 mM trehalose displayed remarkable plant biomass by improving growth physiology, which were supported by the results of chlorophyll fluorescence and rapid light–response curve. In the salinity environment, trehalose + NaCl treatment could greatly inhibit the decrease of malondialdehyde level, and it increases the contents of other osmotic substances, carbohydrates, K+, and K+/Na+ ratio. Meanwhile, trehalose still had similar effects after recovery from salt stress. Furthermore, trehalose pretreatment promoted trehalose metabolism; significantly increased the enzymatic activity of the trehalose metabolic pathway, including trehalose-6-phosphate synthase (TPS), trehalose-6-phosphate phosphatase (TPP), and trehalase (TRE); and upregulated the expression of SlTPS1, SlTPS5, SlTPS7, SlTPPJ, SlTPPH, and SlTRE under saline conditions. However, the transcriptional levels of SlTPS1, SlTPS5, and SlTPS7 genes and the activity of TPS enzyme were reversed after recovery. In addition, we found that hydrogen peroxide (H2O2) and superoxide anion (O2−) were accumulated in tomato leaves because of salt stress, but these parameters were all recovered by foliar-applied trehalose, and its visualization degree was correspondingly reduced. Antioxidant enzyme activities (SOD, POD, and CAT) and related gene expression (SlCu/Zn-SOD, SlFe-SOD, SlMn-SOD, SlPOD, and SlCAT) in salt-stressed tomato leaves were also elevated by trehalose to counteract salt stress. Collectively, exogenous trehalose appeared to be the effective treatment in counteracting the negative effects of salt stress.

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Section: Discussionsupporting

confidence: 58%

Section: Methodsmentioning

confidence: 99%

Trehalose Alleviated Salt Stress in Tomato by Regulating ROS Metabolism, Photosynthesis, Osmolyte Synthesis, and Trehalose Metabolic Pathways

Yang

Yao

et al. 2022

Front. Plant Sci.

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“…This study found that EL significantly increased in pepper seedlings under LL stress, and the RWC of pepper leaves initially decreased and then increased with increasing stress duration. However, the application of Z allowed the mitigation of LL stressed pepper seedlings where the seedlings showed reduced EL and improved RWC ( Figure 1 A,B); similar research on peppers supports this assumption [ 39 ]. This implies that Z may protect cell membrane integrity by increasing the number of unsaturated bonds in membrane lipids.…”

Section: Discussionmentioning

confidence: 60%

“…Low temperature combined with low light (LL) is complex abiotic stress that affects plant biochemistry through photoinhibition and oxidative stress [ 25 , 39 ]. Morphological changes were found in plants when suddenly exposed to unfavorable conditions compared to normal growth.…”

Section: Discussionmentioning

confidence: 99%

Exogenous Zeaxanthin Alleviates Low Temperature Combined with Low Light Induced Photosynthesis Inhibition and Oxidative Stress in Pepper (Capsicum annuum L.) Plants

Ding

Xie

et al. 2022

CIMB

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Low temperature combined with low light (LL) affects crop production, especially the yield and quality of peppers, in northwest China during the winter and spring seasons. Zeaxanthin (Z) is a known lipid protectant and active oxygen scavenger. However, whether exogenous Z can mitigate LL-induced inhibition of photosynthesis and oxidative stress in peppers remains unclear. In this study, we investigated the effects of exogenous Z on photosynthesis and the antioxidant machinery of pepper seedlings subject to LL stress. The results showed that the growth and photosynthesis of pepper seedlings were significantly inhibited by LL stress. In addition, the antioxidant machinery was disturbed by the uneven production and elimination of reactive oxygen species (ROS), which resulted in damage to the pepper. For example, membrane lipid peroxidation increased ROS content, and so on. However, exogenous application of Z before LL stress significantly increased the plant height, stem diameter, net photosynthetic rate (Pn), and stomata, which were obviously closed at LL. The activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), mono de-hydroascorbate reductase (MDHAR), de-hydroascorbate reductase (DHAR), ascorbate peroxidase (APX), and ascorbate oxidase (AAO) improved significantly due to the increased expression of CaSOD, CaCAT, CaAPX, CaMDHAR, and CaDHAR. The ascorbic (AsA) and glutathione (GSH) contents and ascorbic/dehydroascorbate (AsA/DHA) and glutathione/oxidized glutathione (GSH/GSSG) ratios also increased significantly, resulting in the effective removal of hydrogen peroxide (H2O2) and superoxide anions (O2•−) caused by LL stress. Thus, pre-treatment with Z significantly reduced ROS accumulation in pepper seedlings under LL stress by enhancing the activity of antioxidant enzymes and accumulation of components of the ascorbate–glutathione (AsA–GSH) cycle and upregulated key genes in the AsA–GSH cycle.

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“…The chlorophyll fluorescence parameters of cucumber leaves were measured using an FMS-2 pulse-modulated fluorometer (Hansatech Instruments Ltd., Norfolk, England). FS, Fm′ under light adaptation, Fo, Fm, and Fv/Fm under dark adaptation were calculated using the formulae ΦPS II, NPQ, qP, and Fv′/Fm′ ( Tang et al, 2021 ). The contents of photosynthetic pigments (chlorophyll a, chlorophyll b, chlorophyll a + b, and carotenoids) in fresh cucumber leaves were determined ( Arnon, 1949 ), and each sample (0.1 g) was transferred to a 20-ml tube with 10 ml of 80% acetone and then placed in the dark for 48 h (shaken every 8 h) until the leaves turned white.…”

Section: Methodsmentioning

confidence: 99%

Physiological Responses of Cucumber Seedlings to Different Supplemental Light Duration of Red and Blue LED

et al. 2021

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Normal development of plants is inhibited by inadequate light in winter in greenhouses in Northwest China. Growth lamps, using light-emitting diodes (LEDs) with red blue light (7R2B), were used to supplement daylight for 1, 2, and 3 h. Seedling growth, photosynthesis, and photosynthetic product; the Calvin cycle key and sugar metabolism-related enzymes and their encoding genes; and the light signal sensing regulation of key gene expression were studied in greenhouse cucumbers under three treatments to determine the best supplemental light durations to enhance cucumber cultivation in greenhouses in winter. Treatment with LED red and blue light for 3 h significantly promoted the growth and development of cucumbers, root growth, and dry matter accumulation. It improved the photosynthetic rate, photosynthetic pigment content, and light energy utilization efficiency in cucumbers. Supplementation with red and blue LED light for 3 h upregulated the expression levels of key genes encoding the Calvin cycle and enzymes related to sugar metabolism in cucumber leaves, which promoted the synthesis and accumulation of photosynthates. The expression levels of phytochrome B, cryptochrome 1, and hypocotyl 5 in the cucumber leaves were also significantly upregulated after 3 h of light supplementation. Combined LED red and blue light for 3 h should be used to supplement natural light to enhance the cucumber cultivation in greenhouses in winter.

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Alleviating damage of photosystem and oxidative stress from chilling stress with exogenous zeaxanthin in pepper (Capsicum annuum L.) seedlings

Cited by 49 publications

References 57 publications

Trehalose Alleviated Salt Stress in Tomato by Regulating ROS Metabolism, Photosynthesis, Osmolyte Synthesis, and Trehalose Metabolic Pathways

Trehalose Alleviated Salt Stress in Tomato by Regulating ROS Metabolism, Photosynthesis, Osmolyte Synthesis, and Trehalose Metabolic Pathways

Exogenous Zeaxanthin Alleviates Low Temperature Combined with Low Light Induced Photosynthesis Inhibition and Oxidative Stress in Pepper (Capsicum annuum L.) Plants

Physiological Responses of Cucumber Seedlings to Different Supplemental Light Duration of Red and Blue LED

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