Allergy to bovine β‐lactoglobulin: specificity of human IgE to tryptic peptides

Sélo, Isabelle; Clément, Gilles; Bernard, Hervé; Chatel, Jean Marc; Créminon, Christophe; Peltre, Gabriel; Wal, Jean‐Michel

doi:10.1046/j.1365-2222.1999.00612.x

Cited by 205 publications

(163 citation statements)

References 14 publications

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“…24) In pevious studies β-lg allergy was generally considered to be a Th2-dominant chronic inflammatory disease. 25) In our study, increased Th2-cytokine, IgE levels and eosinophils are observed. Recent studies on non-Th1/Th2-cell-based mechanisms for the pathogenesis of allergic diseases identified two additional Th cell subsets, Th17 cells and Treg cells.…”

Section: Discussionsupporting

confidence: 63%

Suppressive Effects of Oral Administration of Heat-Killed <i>Lactobacillus acidophilus</i> on T Helper-17 Immune Responses in a Bovine β-Lactoglobulin-Sensitized Mice Model

Li¹,

Meng²,

Duan³

et al. 2013

Biological & Pharmaceutical Bulletin

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The present study was planned to explore the effect of Lactobacillus (L.) acidophilus on the T helper-17 (Th17) immune response in a mouse model of β-lactoglobulin (β-lg) allergy. Bovine β-lg sensitised BALB/c mice were orally administered with different doses of heat-killed L. acidophilus (low, 5×10 7 colony forming unit (CFU); medium, 5×10 8 CFU; high, 5×10 9 CFU) in 200 µL of phosphate buffered saline (PBS) three times a week, starting from 1 week before β-lg sensitisation for 4 weeks. After the allergen challenge, the numbers of blood eosinophils and neutrophils were examined by light microscope; the levels of cytokine (interleukin (IL)-12, IL-4, tumor growth factor (TGF)-β, IL-10, IL-6 and IL-17A), total immunoglobulin E (IgE) and β-lg-specific IgE contents in the serum were measured with enzyme-linked immunosorbent assay (ELISA); The mRNA expression levels of TGF-β, IL-17A,CD25, Foxp3, retinoic acid-related orphan receptor γt (RORγt) and IL-10 were analyzed using real-time polymerase chain reaction (PCR). The results showed that oral administration of L. acidophilus suppressed hypersensitivity responses, attenuated the numbers of inflammatory cells and inhibited IgE production. We found up-regulation of TGF-β and down-regulation of IL-17A in the serum of L. acidophilus-treated group, along with IL-6 levels was significantly decreased than that of the allergy group (p<0.05). Moreover, the mRNA expression levels of CD25, forkhead box P3 and TGF-β were significantly higher in the spleen of L. acidophilus-treated group, while the mRNA expression levels of IL-17A, RORγt and IL-10 were significantly lower than that in the allergy group (p<0.05). In conclusion, the suppression of major allergic symptoms by oral administration of L.acidophilus was probably due to improve the regulatory T (Treg)/Th17 balance and inhibit the IL-6 production.

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Section: Discussionsupporting

confidence: 63%

Suppressive Effects of Oral Administration of Heat-Killed <i>Lactobacillus acidophilus</i> on T Helper-17 Immune Responses in a Bovine β-Lactoglobulin-Sensitized Mice Model

Li¹,

Meng²,

Duan³

et al. 2013

Biological & Pharmaceutical Bulletin

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“…The site of substitution for BLG coding sequences was thus adequate, since PrtB-BLG and PrtB-T6 were properly processed by the L. lactis secretion machinery and escaped intracellular proteolysis. Anti-BLG antiserum was not reactive, in both ELISA and immunoblot analysis, to T6-expressing clones, confirming previous reports of the low level of antigenicity of this BLG peptide (22,28). The fact that anti-T6 antiserum, which recognizes native BLG in ELISA, failed to react with recombinant L. lactis expressing full-length BLG might reflect either epitope masking or subtle conformational changes in the fusion protein.…”

Section: Discussionsupporting

confidence: 84%

Lactobacillus bulgaricus Proteinase Expressed in Lactococcus lactis Is a Powerful Carrier for Cell Wall-Associated and Secreted Bovine β-Lactoglobulin Fusion Proteins

Bernasconi

Germond

Delley

et al. 2002

Appl Environ Microbiol

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Lactic acid bacteria have a good potential as agents for the delivery of heterologous proteins to the gastrointestinal mucosa and thus for the reequilibration of inappropriate immune responses to food antigens. Bovine ␤-lactoglobulin (BLG) is considered a major allergen in cow's milk allergy. We have designed recombinant Lactococcus lactis expressing either full-length BLG or BLG-derived octapeptide T6 (IDALNENK) as fusions with Lactobacillus bulgaricus extracellular proteinase (PrtB). In addition to constructs encoding fulllength PrtB for the targeting of heterologous proteins to the cell surface, we generated vectors aiming at the release into the medium of truncated PrtB derivatives lacking 100 (PrtB٢, PrtB٢-BLG, and PrtB٢-T6) or 807 (PrtB⌬) C-terminal amino acids. Expression of recombinant products was confirmed using either anti-PrtB, anti-BLG, or anti-peptide T6 antiserum. All forms of the full-length and truncated recombinant products were efficiently translocated, irrespective of the presence of eucaryotic BLG sequences in the fusion proteins. L. lactis expressing PrtB٢-BLG yielded up to 170 g per 10 9 CFU in the culture supernatant and 9 g per 10 9 CFU at the bacterial cell surface within 14 h. Therefore, protein fusions relying on the use of PrtB gene products are adequate for concomitant cell surface display and secretion by recombinant L. lactis and thus may ensure maximal bioavailability of the eucaryotic antigen in the gut-associated lymphoid tissue.An inappropriate immunological response to bovine milk proteins, including ␤-lactoglobulin (BLG), has been put forward as a key factor in cow's milk allergy (34). Studies have shown that a reequilibration of this response toward a state of specific oral tolerance can be achieved upon oral administration of partially hydrolyzed cow's milk formula or BLG peptides obtained by tryptic hydrolysis (8,22). Interestingly, the degradation of cow's milk proteins by lactic acid bacteria (LAB) used in food fermentation was also found to generate tolerogenic peptides, with a suppression of the specific lymphoproliferative response (32). In addition, certain LAB strains are more and more widely used as a vehicle for the delivery of heterologous proteins to the mucosal immune system (13,20). Therefore, it can be hypothesized that the introduction of LAB capable of expressing full-length or BLG fragments may prove them to be a useful tool to aid in host protection against allergic sensitization to this dietary antigen.Lactococcus lactis is a gram-positive, nonpathogenic bacterium that is widely used in industrial food fermentation. A noncolonizing bacterium, L. lactis has been shown to be quite resistant to gastric acidity when administered together with food, remaining metabolically active all the way through the digestive tract (3). Its use as an antigen delivery vehicle for mucosal immunization has been reported (1,4,26,31). Induction of oral tolerance might require a large amount of target antigen, in contrast to immune modulators such as cytokines (30,31). Therefo...

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“…For example, ovomucoid [1] from the egg white, M fraction from fish [2] and beta lactoglobulin [3] from cow's milk have maximum antigenicity comparatively with other antigens from the same foods.…”

mentioning

confidence: 99%

Particularities of the Diet Used in Milk Allergy

Mogos¹,

Dondoi²,

Bajko³

2017

Romanian Journal of Diabetes Nutrition and Metabolic Diseases

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Allergies become more common nowadays because of numerous risk factors and better medical resources for diagnostics. So, it is imperative to have at least an idea how toThrough conservation, culinary processing [4] and digestion, food suffers modifications that can reduce the degree of antigenicity or on the contrary can increase it. Thereby, tomatoes become more allergenic as they become ripe because of incorporating the M-glycosidic fragment in their protein complex [5]. Furthermore, beta lactoglobulins' antigenicity is accentuated through incubation with lactose because of the increase of glycosidic binding reactions responsible for the browning action. Also, heat denaturation amplifies some foods' antigenic capacities (ex. Fish [6]) or diminishes it (ex. boiled milk can be better tolerated if the sensibility is toward heat sensitive proteins [7]).During digestion, following action of enzymatic factors, new antigens can appear that have a different binding capacity than of the original antigens. On the other hand, it must be taken into consideration that through the act of feeding, haptens are introduced in the body. These are incomplete antigenic substances with

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Allergy to bovine β‐lactoglobulin: specificity of human IgE to tryptic peptides

Abstract: Three peptides were identified as major epitopes, recognized by a large majority of human IgE antibodies. Numerous other epitopes are scattered all along the Blg sequence.

Cited by 205 publications

References 14 publications

Suppressive Effects of Oral Administration of Heat-Killed <i>Lactobacillus acidophilus</i> on T Helper-17 Immune Responses in a Bovine β-Lactoglobulin-Sensitized Mice Model

Suppressive Effects of Oral Administration of Heat-Killed <i>Lactobacillus acidophilus</i> on T Helper-17 Immune Responses in a Bovine β-Lactoglobulin-Sensitized Mice Model

Lactobacillus bulgaricus Proteinase Expressed in Lactococcus lactis Is a Powerful Carrier for Cell Wall-Associated and Secreted Bovine β-Lactoglobulin Fusion Proteins

Particularities of the Diet Used in Milk Allergy

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