Alkaline pretreatment methods followed by acid hydrolysis of Saccharum spontaneum for bioethanol production

Chaudhary, Gaurav; Singh, Lalit Kumar; Ghosh, Sanjoy

doi:10.1016/j.biortech.2012.08.067

Cited by 82 publications

(39 citation statements)

References 29 publications

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“…to reduce the lignin content [16]. This pretreatment is normally related with the removal of lignin because NaOH breaks the ester bonds cross-linkage in lignin and xylan [9]. This breaking augments the biomass porosity allowing an easier access of the enzymes to the cellulose.…”

Section: Cellulose Conversionmentioning

confidence: 99%

“…Cellulose conversion and ethanol yield can be improved by modifying the pretreatment's conditions. Several works proved the efficiency of basic hydrolysis to decrease the lignin content in biomass [9]. Additionally, in sweet sorghum bagasse, the combination of alkaline hydrolysis simultaneously with ultrasonication augmented the final ethanol yield and cellulose conversion [10].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of alkali ultrasonication pretreatment in bioethanol production from cotton gin trash using FT-IR spectroscopy and principal component analysis

Plácido

Capareda

2014

Bioresour. Bioprocess.

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Background: Cotton gin trash (CGT) is a lignocellulosic residue that can be used in the production of cellulosic ethanol. In a previous research, the sequential use of ultrasonication, liquid hot water, and ligninolytic enzymes was selected as pretreatment for the production of ethanol from CGT. However, an increment in the ethanol production is necessary. To accomplish that, this research evaluated the effect of pretreating CGT using alkaline ultrasonication before a liquid hot water and ligninolytic enzymes pretreatments for ethanol production. Three NaOH concentrations (5%, 10%, and 15%) were employed for the alkaline ultrasonication. Additionally, this work is one of the first applications of Fourier transform infrared (FT-IR) spectrum and principal component analysis (PCA) as fast methodology to identify the differences in the biomass after different types of pretreatments. Results: The three concentrations employed for the alkaline ultrasonication pretreatment produced ethanol yields and cellulose conversions higher than the experiment without NaOH. Furthermore, 15% NaOH concentration achieved twofold increment yield versus the treatment without NaOH. The FT-IR spectrum confirmed modifications in the CGT structure in the different pretreatments. PCA was helpful to determine differences between the pretreated and un-pretreated biomass and to evaluate how the CGT structure changed after each treatment. Conclusions: The combination of alkali ultrasonication hydrolysis, liquid hot water, and ligninolytic enzymes using 15% of NaOH improved 35% the ethanol yield compared with the original treatment. Additionally, we demonstrated the use of PCA to identify the modifications in the biomass structure after different types of pretreatments and conditions.

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Section: Cellulose Conversionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of alkali ultrasonication pretreatment in bioethanol production from cotton gin trash using FT-IR spectroscopy and principal component analysis

Plácido

Capareda

2014

Bioresour. Bioprocess.

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“…The application of acid hydrolysis involves the possibility of the transformation of sugars (including pentoses) to furfural and levulinic acid, and to 5-HMF in the case of glucose [23,39]. Partial hydrolysis of lignin as a result of which phenolic compounds pass into the hydrolysate solution is also possible.…”

Section: Influence Of the Pre-treatment And Saccharification Conditiomentioning

confidence: 99%

Comparison and Optimization of Saccharification Conditions of Alkaline Pre-Treated Triticale Straw for Acid and Enzymatic Hydrolysis Followed by Ethanol Fermentation

et al. 2018

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This paper concerns the comparison of the efficiency of two-stage hydrolysis processes, i.e., alkaline pre-treatment and acid hydrolysis, as well as alkaline pre-treatment followed by enzymatic hydrolysis, carried out in order to obtain reducing sugars from triticale straw. For each of the analyzed systems, the optimization of the processing conditions was carried out with respect to the glucose yield. For the alkaline pre-treatment, an optimal catalyst concentration was selected for constant values of temperature and pre-treatment time. For enzymatic hydrolysis, optimal process time and concentration of the enzyme preparation were determined. For the acidic hydrolysis, performed with 85% phosphoric acid, the optimum temperature and hydrolysis time were determined. In the hydrolysates obtained after the two-stage treatment, the concentration of reducing sugars was determined using HPLC. The obtained hydrolysates were subjected to ethanol fermentation. The concentrations of fermentation inhibitors are given and their effects on the alcoholic fermentation efficiency are discussed.

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“…Lignin is a protective physical barrier to enzyme attack. Under alkaline conditions, lignin is almost completely removed from the lignocellulosic biomass (Chaudhary et al 2012). To facilitate the rapid and efficient hydrolysis of carbohydrates, alkaline delignification pretreatment was used in this study.…”

Section: Composition Changes After Pretreatmentmentioning

confidence: 99%

Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane Bagasse

Liu¹,

Xu²,

Zhang³

et al. 2016

BioResources

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Alkali-pretreated sugarcane bagasse fiber was subjected to fed-batch simultaneous saccharification and fermentation (SSF) with a prehydrolysis process to increase the solids loading and produce a high concentration of ethanol. The hydrolysis medium and yeast feeding modes were investigated to determine suitable conditions for high sugar yield and ethanol production. Batch addition resulted in a cumulative substrate concentration of up to 36% (w/v) and enhanced ethanol concentrations, while ethanol conversion efficiency gradually declined. Enzymatic prehydrolysis and fermentation with fed-batch mode contributed to the SSF process. The highest ethanol concentration was 66.915 g/L with the conversion efficiency of 72.89%, which was achieved at 30% (w/v) solids content after 96 h of fermentation. Hydrolyzed medium and yeast were added in batch mode at 24 h of enzymatic hydrolysis and fermentation, respectively. Thus, combining the fed-batch mode with pre-hydrolysis SSF produced a high yield of ethanol.

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Alkaline pretreatment methods followed by acid hydrolysis of Saccharum spontaneum for bioethanol production

Cited by 82 publications

References 29 publications

Analysis of alkali ultrasonication pretreatment in bioethanol production from cotton gin trash using FT-IR spectroscopy and principal component analysis

Analysis of alkali ultrasonication pretreatment in bioethanol production from cotton gin trash using FT-IR spectroscopy and principal component analysis

Comparison and Optimization of Saccharification Conditions of Alkaline Pre-Treated Triticale Straw for Acid and Enzymatic Hydrolysis Followed by Ethanol Fermentation

Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane Bagasse

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