Alkaline phosphatase is involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1

Ali, Aus Tariq; Penny, Clem; Paiker, Janice; Niekerk, Chantal van; Smit, A. M.; Ferris, William F.; Crowther, Nigel J.

doi:10.1016/j.cccn.2004.11.026

Cited by 69 publications

(79 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…9,10 The present study has confirmed the parallel relationship between elevated ALP enzyme activity and cellular fat accumulation in abdominal subcutaneous adipose tissue. The intracellular deposition of lipid is the defining feature of the maturation of preadipocytes into adipocytes, a process termed "adipogenesis".…”

Section: Discussionsupporting

confidence: 85%

“…Results from 3T3-L1 cells using ALP inhibitors and PCR demonstrate that it is the tissue-non-specific isoform that is expressed in this cell type. 9 The ability of levamisole to increase ALP activity in human preadipocytes has been reported previously, 10 and a study of rat hepatocytes, which are known to express the tissue-non-specific ALP isoform, also showed a similar effect. 22 It should be noted that levamisole inhibits ALP activity in cell extracts of human preadipocytes.…”

Section: Discussionsupporting

confidence: 56%

“…For example, preadipocytes that are isolated from the visceral depot differentiate more slowly, compared with subcutaneous preadipocytes. 8 Previously, we demonstrated a positive correlation between ALP activity and fat accumulation in murine 3T3-L1 preadipocytic cells 9 and human mammary gland preadipocytes. 10 The ability of ALP inhibitors to pharmacologically inhibit intracellular lipid accumulation suggests that ALP may play a role in preadipocyte lipid storage.…”

Section: Introductionmentioning

confidence: 89%

“…These doses were based on a previous study using the murine 3T3-L1 preadipocyte cell line. 9 ALP activity and intracellular lipid accumulation were measured before intracellular lipid accumulation was initiated (day 0), and 12 days after stimulation of adipogenesis in preadipocytes isolated from all study subjects. These variables were also measured in the presence of the ALP inhibitors, but not in all subjects.…”

Section: Alkaline Phosphatase Inhibitorsmentioning

confidence: 99%

“…10 The ability of ALP inhibitors to pharmacologically inhibit intracellular lipid accumulation suggests that ALP may play a role in preadipocyte lipid storage. 9,10 It is not known whether ALP is expressed in other body fat depots, or whether it is involved in the molecular control of adipogenesis at such sites. Abdominal subcutaneous adipose tissue is an important fat storage depot and may influence whole body insulin sensitivity.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Lipid accumulation and alkaline phosphatase activity in human preadipocytes isolated from different body fat depots

Ali

Ferris

Penny

et al. 2013

Journal of Endocrinology, Metabolism and Diabetes of South Afri

View full text Add to dashboard Cite

Background: Alkaline phosphatase (ALP) controls intracellular lipid accumulation in human preadipocytes, but it is not known whether ALP is expressed in all body fat depots, or whether it has a similar role at all sites.Design: Cross-sectional.Setting and subjects: Subjects undergoing breast reduction and abdominal fat biopsies operations at Charlotte Maxeke Johannesburg Academic Hospital. Outcome measures:This study compared intracellular lipid accumulation and ALP activity in the presence and absence of ALP inhibitors in preadipocytes that were obtained from different adipose depots. Abdominal and mammary gland preadipocytes were isolated from women and induced to differentiate in culture. ALP activity and intracellular lipid levels were measured at baseline and after 12 days of differentiation in the presence and absence of the ALP inhibitors, histidine and levamisole.Results: ALP activity was detected in nondifferentiated abdominal (134 ± 7.5 mU/mg protein) and mammary gland (136 ± 9.6 mU/mg protein) preadipocytes. Its activity had increased significantly (p-value < 0.0005 for both) by day 12 of differentiation (388 ± 55 for abdominal and 278 ± 28 mU/mg protein for mammary). Preadipocytes treated with histidine had lower fat accumulation (p-value < 0.0005) and ALP activity (p-value < 0.005) than nontreated cells on day 12, while those treated with levamisole had lower fat accumulation (p-value < 0.005), but elevated ALP activity (p-value < 0.05), compared to nontreated cells. Lipid accumulation (p-value < 0.005) and ALP activity (p-value < 0.05) were higher in abdominal than mammary gland preadipocytes by day 12.Conclusion: ALP is involved in the control of intracellular lipid accumulation in human preadipocytes that are isolated from both adipose depots. The ability of levamisole to inhibit this process while activating ALP, suggests that this molecule acts via an ALP-independent pathway, while histidine attenuates both lipid deposition and ALP activity.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 89%

Section: Alkaline Phosphatase Inhibitorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Lipid accumulation and alkaline phosphatase activity in human preadipocytes isolated from different body fat depots

Ali

Ferris

Penny

et al. 2013

Journal of Endocrinology, Metabolism and Diabetes of South Afri

View full text Add to dashboard Cite

show abstract

In Situ Localization of Enzyme Activity in Live Cells by a Molecular Probe Releasing a Precipitating Fluorochrome

Liu

et al. 2017

Angewandte Chemie

View full text Add to dashboard Cite

Current enzyme-responsive,f luorogenic probes fail to provide in situ information because the released fluorophores tend to diffuse away from the reaction sites.T he problem of diffusive signal dilution can be addressed by designing ap robe that upon enzyme conversion releases afluorophore that precipitates.Anexcited-state intramolecular proton transfer (ESIPT)-based solid-state fluorophore HTPQ was developed that is strictly insoluble in water and emits intense fluorescence in the solid state,with l ex/em = 410/550 nm, thus making it far better suited to use with ac ommercial confocal microscope.HTPQ was further utilized in the design of an enzyme-responsive,f luorogenic probe (HTPQA), targeting alkaline phosphatase (ALP) as am odel enzyme. HTPQA makes possible diffusion-resistant in situ detection of endogenous ALP in live cells.I tw as also employed in the visualizing of different levels of ALP in osteosarcoma cells and tissue,t hus demonstrating its interest for the diagnosis of this type of cancer.

show abstract

An Activatable AIEgen Probe for High‐Fidelity Monitoring of Overexpressed Tumor Enzyme Activity and Its Application to Surgical Tumor Excision

Yao

et al. 2020

Angewandte Chemie

View full text Add to dashboard Cite

Monitoring fluctuations in enzyme overexpression facilitates early tumor detection and excision. An AIEgen probe (DQM‐ALP) for the imaging of alkaline phosphatase (ALP) activity was synthesized. The probe consists of a quinoline‐malononitrile (QM) core decorated with hydrophilic phosphate groups as ALP‐recognition units. The rapid liberation of DQM‐OH aggregates in the presence of ALP resulted in aggregation‐induced fluorescence. The up‐regulation of ALP expression in tumor cells was imaged using DQM‐ALP. The probe permeated into 3D cervical and liver tumor spheroids for imaging spatially heterogeneous ALP activity with high spatial resolution on a two‐photon microscopy platform, providing the fluorescence‐guided recognition of sub‐millimeter tumorigenesis. DQM‐ALP enabled differentiation between tumor and normal tissue ex vivo and in vivo, suggesting that the probe may serve as a powerful tool to assist surgeons during tumor resection.

show abstract

Alkaline phosphatase is involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1

Cited by 69 publications

References 45 publications

Lipid accumulation and alkaline phosphatase activity in human preadipocytes isolated from different body fat depots

Lipid accumulation and alkaline phosphatase activity in human preadipocytes isolated from different body fat depots

In Situ Localization of Enzyme Activity in Live Cells by a Molecular Probe Releasing a Precipitating Fluorochrome

An Activatable AIEgen Probe for High‐Fidelity Monitoring of Overexpressed Tumor Enzyme Activity and Its Application to Surgical Tumor Excision

Contact Info

Product

Resources

About