Alishewanella solinquinati sp. nov., Isolated from Soil Contaminated with Textile Dyes

Abstract

A novel Gram-negative, motile, rod-shaped, facultative anaerobic bacterial strain, KMK6(T), was isolated from soil contaminated with textile dyes from an industrial estate located at Ichalkaranji, Maharashtra, India, and its taxonomical position was established by using a polyphasic approach. The major cellular fatty acids included C17:1ω8c, summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), C17:0, C16:0, and C18:1ω7c. The DNA G+C content of strain KMK6(T) was 48.8 mol %. 16S rRNA gene sequence analysis confirmed its placement in the genus Alishewanella, and exhibited sequence similarity levels of below 97 % to the type strains of validly published Alishewanella species. On the basis of genotypic and phenotypic evidence, strains KMK6(T) is considered to be a novel species of the genus Alishewanella, for which we propose that strain KMK6(T) (=NCIM 5295(T) =BCRC 17848(T)) is assigned to a novel species, Alishewanella solinquinati sp. nov.

Application of Ion Torrent Sequencing to the Assessment of the Effect of Alkali Ballast Water Treatment on Microbial Community Diversity

“…SG13. NCBI#:HQ413096), a human fetus [55], tidal sediments [56], fermented seafood [57], landfills [58], textile dye contaminated soils [59], and industrial effluents [60]. According to a previous study by Kim et al 2010 [58], Alishewanella agri BL06 can grow in the pH range between 5.5 and 12.0.…”

Optimized production, characterization and application of alkaline proteases from taxonomically assessed microbial isolates from Lonar soda lake, India

“…Isolates were screened for alkaline protease production by using skimmed milk agar (pH 10.0; NaCl 1%) plates (Pathak and Rathod, 2013). Morphological, microscopic and physiological characters, enzyme profile, sugar utilization and antibiotic susceptibility pattern of the selected isolates were determined (Sneath et al, 1986;Vos et al, 2009;Khan et al, 2009;Kolekar et al, 2013;Sardar and Pathak, 2014;Pathak and Rathod, 2015c;Pathak et al, 2015a,b,c,d,e). The selected isolates were subjected for the 16S rRNA partial gene sequencing as per the methods described previously by Rathod and Pathak, (2014a).…”

Draft Whole-Genome Sequence of the Alkaliphilic Alishewanella aestuarii Strain HH-ZS, Isolated from Historical Lime Kiln Waste-Contaminated Soil

“…The genus has received further attention following isolations from tidal flats (2), mountainous lakes (3), landfill soils (4), industrially contaminated soils (5), and traditional fermented foods (6). This genus has received particular attention because of its bioremediation potential and broad temperature and pH ranges for growth (7–9).…”

“…The genome has a G+C content of 51.0% and contains 68 genes encoding rRNA (5S, 16S, 23S), 60 tRNA, and five ncRNA. RAST system annotation assigned proteins to stress response, metal resistance (As, Cd, Co, Cr, Zn) and tolerance (Cu), metabolism of carbohydrates, and aromatic compounds associated with contaminated and high-pH environments (5, 7, 10). …”