Algae in a phosphorus‐limited landscape

Grossman, Arthur R.; Aksoy, Munevver

doi:10.1002/9781118958841.ch12

Cited by 16 publications

(30 citation statements)

References 216 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…a). This is approximately the same number of Pi transporters as annotated in Arabidopsis thaliana (The Arabidopsis Information Ressource, TAIR, database), whereas Chlamydomonas reinhardtii has 14 annotated putative Pi transporters (Grossman & Aksoy, ). Twelve of the Pi transporter genes in P. tricornutum were highly upregulated under Pi depletion, with 11 being dramatically downregulated on Pi resupply (Fig.…”

Section: Resultsmentioning

confidence: 99%

Noncoding and coding transcriptome responses of a marine diatom to phosphate fluctuations

et al. 2015

View full text Add to dashboard Cite

SummaryPhosphorus (P) is an essential element to all living cells, yet fluctuations in P concentrations are recurrent in the marine environment. Diatoms are amongst the most successful phytoplankton groups, adapting to and surviving periods of suboptimal conditions and resuming growth as soon as nutrient concentrations permit. A knowledge of the molecular underpinnings of diatom ecological success is, however, still very incomplete.By strand-specific RNA sequencing, we analyzed the global transcriptome changes of the diatom Phaeodactylum tricornutum in response to P fluctuations over a course of 8 d, defining five distinct physiological states.This study reports previously unidentified genes highly responsive to P stress in P. tricornutum. Our data also uncover the complexity of the P. tricornutum P-responsive sensory and signaling system that combines bacterial two-component systems with more complex pathways reminiscent of metazoans. Finally, we identify a multitude of novel long intergenic nonprotein coding RNAs (lincRNAs) specifically responsive to P depletion, suggesting putative regulatory roles in the regulation of P homeostasis.Our work provides additional molecular insights into the resilience of diatoms and their ecological success, and opens up novel routes to address and explore the function and regulatory roles of P. tricornutum lincRNAs in the context of nutrient stress.

show abstract

Section: Resultsmentioning

confidence: 99%

Noncoding and coding transcriptome responses of a marine diatom to phosphate fluctuations

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Importantly, phosphate may be a strong limiting factor for phytoplankton growth in the field ( Alexander et al. 2015 ; Grossman and Aksoy 2015 ; Lin et al. 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Inter- and Intra-Specific Transcriptional and Phenotypic Responses of Pseudo-nitzschia under Different Nutrient Conditions

Lema

Metegnier

Quéré

et al. 2019

Genome Biology and Evolution

View full text Add to dashboard Cite

Untangling the functional basis of divergence between closely related species is a step toward understanding species dynamics within communities at both the evolutionary and ecological scales. We investigated cellular (i.e., growth, domoic acid production, and nutrient consumption) and molecular (transcriptomic analyses) responses to varying nutrient concentrations across several strains belonging to three species of the toxic diatom genus Pseudo-nitzschia. Three main results were obtained. First, strains from the same species displayed similar transcriptomic, but not necessarily cellular, responses to the experimental conditions. It showed the importance of considering intraspecific diversity to investigate functional divergence between species. Second, a major exception to the first finding was a strain recently isolated from the natural environment and displaying contrasting gene expression patterns related to cell motility and domoic acid production. This result illustrated the profound modifications that may occur when transferring a cell from the natural to the in vitro environment and asks for future studies to better understand the influence of culture duration and life cycle on expression patterns. Third, transcriptomic responses were more similar between the two species displaying similar ecology in situ, irrespective of the genetic distance. This was especially true for molecular responses related to TCA cycle, photosynthesis, and nitrogen metabolism. However, transcripts related to phosphate uptake were variable between species. It highlighted the importance of considering both overall genetic distance and ecological divergence to explain functional divergence between species.

show abstract

“…The effects of P limitation or P starvation on most pathways of the carbon metabolism may be viewed as secondary. P-specific responses are strong and robust, and are consistently observed across photosynthetic organisms [ 23 ]. Responses of the carbon metabolism pathways, however, are variable between different studies, making general observations on these responses difficult.…”

Section: Carbon Metabolism Responses To Phosphorus Limitation In Diatmentioning

confidence: 99%

“…A number of reviews in the last few years have provided updated knowledge on the biogeochemical P cycle [ 2 , 3 ] as well as physiological and molecular mechanisms of P uptake, metabolism and storage in microalgae [ 5 , 23 , 24 ]. Here, we review the effect of P availability on carbon metabolism pathways in diatoms, using two transcriptome analyses as a basis, supported by other studies at transcriptome, proteome and metabolite levels.…”

Section: Introductionmentioning

confidence: 99%

The effects of phosphorus limitation on carbon metabolism in diatoms

Brembu¹,

Mühlroth²,

Alipanah³

et al. 2017

Phil. Trans. R. Soc. B

113

View full text Add to dashboard Cite

Phosphorus is an essential element for life, serving as an integral component of nucleic acids, lipids and a diverse range of other metabolites. Concentrations of bioavailable phosphorus are low in many aquatic environments. Microalgae, including diatoms, apply physiological and molecular strategies such as phosphorus scavenging or recycling as well as adjusting cell growth in order to adapt to limiting phosphorus concentrations. Such strategies also involve adjustments of the carbon metabolism. Here, we review the effect of phosphorus limitation on carbon metabolism in diatoms. Two transcriptome studies are analysed in detail, supplemented by other transcriptome, proteome and metabolite data, to gain an overview of different pathways and their responses. Phosphorus, nitrogen and silicon limitation responses are compared, and similarities and differences discussed. We use the current knowledge to propose a suggestive model for the carbon flow in phosphorus-replete and phosphorus-limited diatom cells.This article is part of the themed issue ‘The peculiar carbon metabolism in diatoms’.

show abstract

Algae in a phosphorus‐limited landscape

Cited by 16 publications

References 216 publications

Noncoding and coding transcriptome responses of a marine diatom to phosphate fluctuations

Noncoding and coding transcriptome responses of a marine diatom to phosphate fluctuations

Inter- and Intra-Specific Transcriptional and Phenotypic Responses of Pseudo-nitzschia under Different Nutrient Conditions

The effects of phosphorus limitation on carbon metabolism in diatoms

Contact Info

Product

Resources

About