Alendronate delivery on amino modified mesoporous bioactive glass scaffolds to enhance bone regeneration in osteoporosis rats

Wang, Xiao; Zeng, Deliang; Weng, Wei-min; Huang, Qing-Guo; Zhang, Xiangkai; Wen, Ji; Wu, Jiannan; Jiang, Xinquan

doi:10.1080/21691401.2018.1453825

Cited by 34 publications

(31 citation statements)

References 39 publications

(51 reference statements)

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“…isoprenoid biosynthesis and subsequent protein prenylation to inhibit cell activity [33]. e administration of bioactive glass associated with risedronate to the osteoporotic bone induces the reconnection of bone trabecular.…”

Section: Bone Quality and Quantity: Assessment By μCtmentioning

confidence: 99%

Risedronate Effects on the In Vivo Bioactive Glass Behavior: Nuclear Magnetic Resonance and Histopathological Studies

Mosbahi

Oudadesse

Roiland

et al. 2019

BioMed Research International

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e present study aimed to enhance the anti-osteoporotic performance of bioactive glass (46S6) through its association with bisphosphonate such as risedronate with amounts of 8, 12, and 20%. Obtained composites have been called 46S6-8RIS, 46S6-12RIS, and 46S6-20RIS, respectively. In vitro and in vivo explorations have been carried out. Bioactive glass and risedronate association has been performed by adsorption process. Structure analyses have been carried out to evaluate and to understand their chemical interactions. Solid Nuclear Magnetic Resonance (NMR) has been employed to study the structural properties of obtained biocomposite. e spectra deconvolution showed the appearance of a species ( 4 ) in the biocomposites 46S6-8RIS, 46S6-12RIS, and 46S6-20RIS indicating their successful chemical association. In vitro experiments showed the enhancement of the chemical reactivity of the composites 46S6-xRIS compared to the pure bioactive glass. In fact, the silicon liberation a er 30 days of immersion was 50 ppm for pure bioactive glass 46S6, and 41, 64, and 62 from 46S6-8RIS, 46S6-12RIS, and 46S6-20RIS, respectively. Based on the in vitro results, 46S6-8RIS was implanted in the femoral condyle of an ovariectomized rat and compared with implanted pure glass in the goal to highlight its anti-osteoporotic performance. A er 60 days, implanted group with 46S6-8RIS showed the increase in bone mineral density (BMD with 10%) and bone volume fraction (BV/TV with 80%) and the decrease in trabecular separation (Tb/Sp with 74%) when compared to that of 46S6 group. ese results are con rmed by the histopathological analyses, which showed the bone trabeculae reconnection a er the 46S6-8RIS implantation. Chemical analyses showed the reduction in silicon (Si) and sodium (Na) ion concentrations, and the rise in calcium (Ca) and phosphorus (P) ion levels, which was explained by the dissolution of biocomposite matrix and the deposition of hydroxyapatite layer. Histomorphometric results highlighted the risedronate e ect on the antiosteoporotic phenomenon. Obtained results showed good behavior with only 8% of introduced risedronate in the glass matrix.

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Section: Bone Quality and Quantity: Assessment By μCtmentioning

confidence: 99%

Risedronate Effects on the In Vivo Bioactive Glass Behavior: Nuclear Magnetic Resonance and Histopathological Studies

Mosbahi

Oudadesse

Roiland

et al. 2019

BioMed Research International

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“…Many clinical studies have been performed on calvarial defect models to evaluate bone regeneration related to different diseases (43)(44)(45)(46). Histological techniques have been used to demonstrate tissue damage (47)(48)(49).…”

Section: Discussionmentioning

confidence: 99%

Effects of alloplastic graft material combined with a topical ozone application on calvarial bone defects in rats

et al. 2020

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Background:The present study presents the evaluation of the damage in the bone tissue resulting from a calvarial defect in rats and the efficiency of exposure to an ozone application with an alloplastic bone graft on the calvarial bone damage. Materials and methods:Wistar male rats (n = 56) were divided into four groups: a control group (n = 14), defect and ozone group (n = 14), defect and graft group (n = 14), and defect, graft, and ozone group (n = 14). Under anaesthesia, a circular full-thickness bone defect was created in all groups, and the experimental groups were further divided into two sub-groups, with seven rats in each group sacrificed at the end of the 4 th and 8 th weeks. Bone samples were dissected, fixed in 10% formalin solution, and decalcified with 5% ethylene-diaminetetraacetic acid (EDTA). After the routine follow-up on tissues, immunostaining of osteopontin and osteonectin antibodies was applied to sections and observed under a light microscope. Results:The control group exhibited osteopontin and osteonectin expression in fibroblasts and inflammatory cells at the end of the 4 th week with an acceleration at the 8 th week. Ozone administration elucidated new trabecular bone formation by increasing osteoblastic activity.Lastly, our observations underscore that a combination of allograft and ozone application increased the osteoblast, osteocyte, and bone matrix development at the 4 th and 8 th weeks. Conclusions:Exposure to an ozone application with an alloplastic bone graft on calvarial bone damage may induce osteoblastic activity, matrix development, mature bone cell formation, and new bone formation in rats.

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“…Postoperatively, penicillin was administered to reduce the risk of inflammation. 29 OVX BMSC isolation and culture OVX BMSCs were harvested from the ovariectomized rats 3 months after ovariectomy. Briefly, the osteoporotic rats were sacrificed, and both ends of the femora were cut off to expose the medullary canal.…”

Section: Fabrication Of Materials and Characterization Measurementsmentioning

confidence: 99%

Study of Sr–Ca–Si-based scaffolds for bone regeneration in osteoporotic models

Wang

Jiang

et al. 2020

Int J Oral Sci

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Bone tissue engineering has emerged as a promising alternative therapy for patients who suffer bone fractures or defects caused by trauma, congenital diseases or tumours. However, the reconstruction of bone defects combined with osteoporosis remains a great challenge for clinicians and researchers. Based on our previous study, Ca–Si-based bioceramics (MSCs) showed enhanced bone formation capabilities under normal conditions, and strontium was demonstrated to be therapeutic in promoting bone quality in osteoporosis patients. Therefore, in the present study, we attempted to enlarge the application range of MSCs with Sr incorporation in an osteoporotic bone regeneration model to evaluate whether Sr could assist in regeneration outcomes. In vitro readout suggested that Sr-incorporated MSC scaffolds could enhance the expression level of osteogenic and angiogenic markers of osteoporotic bone mesenchymal stem cells (OVX BMSCs). Animal experiments showed a larger new bone area; in particular, there was a tendency for blood vessel formation to be enhanced in the Sr-MSC scaffold group, showing its positive osteogenic capacity in bone regeneration. This study systematically illustrated the effective delivery of a low-cost therapeutic Sr agent in an osteoporotic model and provided new insight into the treatment of bone defects in osteoporosis patients.

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Alendronate delivery on amino modified mesoporous bioactive glass scaffolds to enhance bone regeneration in osteoporosis rats

Cited by 34 publications

References 39 publications

Risedronate Effects on the In Vivo Bioactive Glass Behavior: Nuclear Magnetic Resonance and Histopathological Studies

Risedronate Effects on the In Vivo Bioactive Glass Behavior: Nuclear Magnetic Resonance and Histopathological Studies

Effects of alloplastic graft material combined with a topical ozone application on calvarial bone defects in rats

Study of Sr–Ca–Si-based scaffolds for bone regeneration in osteoporotic models

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