2017
DOI: 10.1590/s0102-865020170110000010 View full text |Buy / Rent full text
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Abstract: Purpose: To investigate the use Aldefluor ® and N, N -Dimethylaminobenzaldehyde (DEAB) to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. Methods: Activated Aldefluor ® aliquots were prepared and maintained at temperature between 2 to 8°C, or stored at -20°C. Next, the cells were collected following the standard protocol of sample preparation. Results: Best results were obtained with Aldefluor ® 1.5µl and DEAB 15 µl for 1 x 10 6 cells, incubated at 37°C for 1… Show more

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