Alantolactone induces concurrent apoptosis and GSDME-dependent pyroptosis of anaplastic thyroid cancer through ROS mitochondria-dependent caspase pathway

Hu, Yiqun; Qi, Wen; Cai, Yefeng; Liu, Yunye; Ma, Wenli; Li, Qinglin; Song, Fahuan; Guo, Yawen; Zhu, Lei; Ge, Jingyan; Zeng, Qian; Wang, Jiahui; Yin, Changtian; Zheng, Guowan; Ge, Minghua

doi:10.1016/j.phymed.2022.154528

Cited by 32 publications

(21 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, cell apoptosis can also be classified into caspase‐dependent and cell‐independent pathways. Many anticancer drugs also induced cell apoptosis via mitochondria‐mediated effects and reduced mitochondria membrane potential, causing cytochrome c release from mitochondria and promoting caspase‐9 and ‐3 activations 48,49 . From these results, the combination of IRI with PEITC promoted apoptotic cell death may be through caspase signaling pathways.…”

Section: Discussionmentioning

confidence: 95%

Phenethyl isothiocyanate and irinotecan synergistically induce cell apoptosis in colon cancer HCT 116 cells in vitro

Lai,

Chueh,

et al. 2023

Environmental Toxicology

View full text Add to dashboard Cite

Irinotecan (IRI), an anticancer drug to treat colon cancer patients, causes cytotoxic effects on normal cells. Phenethyl isothiocyanate (PEITC), rich in common cruciferous plants, has anticancer activities (induction of cell apoptosis) in many human cancer cells, including colon cancer cells. However, the anticancer effects of IRI combined with PEITC on human colon cancer cells in vitro were unavailable. Herein, the aim of this study is to focus on the apoptotic effects of the combination of IRI and PEITC on human colon cancer HCT 116 cells in vitro. Propidium iodide (PI) exclusion and Annexin V/PI staining assays showed that IRI combined with PEITC decreased viable cell number and induced higher cell apoptosis than that of IRI or PEITC only in HCT 116 cells. Moreover, combined treatment induced higher levels of reactive oxygen species (ROS) and Ca2+ than that of IRI or PEITC only. Cells pre‐treated with N‐acetyl‐l‐cysteine (scavenger of ROS) and then treated with IRI, PEITC, or IRI combined with PEITC showed increased viable cell numbers than that of IRI or PEITC only. IRI combined with PEITC increased higher caspase‐3, ‐8, and ‐9 activities than that of IRI or PEITC only by flow cytometer assay. IRI combined with PEITC induced higher levels of ER stress‐, mitochondria‐, and caspase‐associated proteins than that of IRI or PEITC treatment only in HCT 116 cells. Based on these observations, PEITC potentiates IRI anticancer activity by promoting cell apoptosis in the human colon HCT 116 cells. Thus, PEITC may be a potential enhancer for IRI in humans as an anticolon cancer drug in the future.

show abstract

Section: Discussionmentioning

confidence: 95%

Phenethyl isothiocyanate and irinotecan synergistically induce cell apoptosis in colon cancer HCT 116 cells in vitro

Lai,

Chueh,

et al. 2023

Environmental Toxicology

View full text Add to dashboard Cite

show abstract

“…This malignancy poses a serious threat to human health and is currently resistant to effective treatment. An increasing number of studies on ATC, including targeted therapy, , immunotherapy, , nanomedicines, and Chinese medicine therapy, , have shown encouraging results. Because herbal medicines and monomers have a wide range of sources, their research prospects for treating ATC are substantial.…”

Section: Discussionmentioning

confidence: 99%

“…The use of Chinese herbs for treating ATC has recently increased. , Digitalis has been employed for centuries to treat heart failure and arrhythmias and remains one of the most effective medications. Recent findings have suggested that the Digitalis extract has significant antitumor efficacy against various malignant tumors, including cholangiocarcinoma, lung cancer, cervical cancer, pancreatic cancer, and multiple myeloma. − However, the main components of Digitalis and the molecular mechanisms by which it inhibits ATC are poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Incorporating Network Pharmacology and Experimental Validation to Identify Bioactive Compounds and Potential Mechanisms of Digitalis in Treating Anaplastic Thyroid Cancer

Zhu,

Liang,

Guo

et al. 2024

ACS Omega

Self Cite

View full text Add to dashboard Cite

Anaplastic thyroid cancer (ATC) is one of the most lethal malignant tumors for which there is no effective treatment. There are an increasing number of studies on herbal medicine for treating malignant tumors, and the classic botanical medicine Digitalis and its active ingredients for treating heart failure and arrhythmias have been revealed to have significant antitumor efficacy against a wide range of malignant tumors. However, the main components of Digitalis and the molecular mechanisms of its anti-ATC effects have not been extensively studied. Here, we screened the main components and core targets of Digitalis and verified the relationship between the active components and targets through network pharmacology, molecular docking, and experimental validation. These experiments showed that the active ingredients of Digitalis inhibit ATC cell activity and lead to ATC cell death through the apoptotic pathway.

show abstract

“…Pytoptosis, also termed as inflammation-related cell death, is an important natural immune response of the body ( Hu et al, 2022 ; Naryzhnaya et al, 2022 ). Although there are no researches reporting the possible relationships between pyroptosis and curcumin alleviating IRI yet, the inhibition of pyroptosis has been confirmed to mitigate IRI.…”

Section: Curcumin and Pyroptosis In Irimentioning

confidence: 99%

Role of curcumin in ischemia and reperfusion injury

Zhang

2023

Front. Pharmacol.

View full text Add to dashboard Cite

Ischemia-reperfusion injury (IRI) is an inevitable pathological process after organic transplantations. Although traditional treatments restore the blood supply of ischemic organs, the damage caused by IRI is always ignored. Therefore, the ideal and effective therapeutic strategy to mitigate IRI is warrented. Curcumin is a type of polyphenols, processing such properties as anti-oxidative stress, anti-inflammation and anti-apoptosis. However, although many researches have been confirmed that curcumin can exert great effects on the mitigation of IRI, there are still some controversies about its underlying mechanisms among these researches. Thus, this review is to summarize the protective role of curcumin against IRI as well as the controversies of current researches, so as to clarify its underlying mechanisms clearly and provide clinicians a novel idea of the therapy for IRI.

show abstract

Alantolactone induces concurrent apoptosis and GSDME-dependent pyroptosis of anaplastic thyroid cancer through ROS mitochondria-dependent caspase pathway

Cited by 32 publications

References 46 publications

Phenethyl isothiocyanate and irinotecan synergistically induce cell apoptosis in colon cancer HCT 116 cells in vitro

Phenethyl isothiocyanate and irinotecan synergistically induce cell apoptosis in colon cancer HCT 116 cells in vitro

Incorporating Network Pharmacology and Experimental Validation to Identify Bioactive Compounds and Potential Mechanisms of Digitalis in Treating Anaplastic Thyroid Cancer

Role of curcumin in ischemia and reperfusion injury

Contact Info

Product

Resources

About