Alanine-Threonine Polymorphism of
            <i>Helicobacter pylori</i>
            RpoB Is Correlated with Differential Induction of Interleukin-8 in MKN45 Cells

Lee, Keun Hwa; Cho, Myung Je; Yamaoka, Yoshio; Graham, David Y.; Yun, Yong Ju; Woo, So Yon; Lim, Chang Young; Ko, Kwan Soo; Kim, Bum Joon; Jung, Hyun Chae; Lee, Woo Kon; Rhee, Kwang Ho; Kook, Yoon Hoh

doi:10.1128/jcm.42.8.3518-3524.2004

Cited by 13 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These observations demonstrate that gastric cells expressing a1,4-GlcNAc-capped Oglycans protect themselves against Hp infection. Our results showed no expression of terminal a1,4-GlcNAc with the gastric carcinoma cell lines studied ( Table 2), demonstrating that the cell lines used in the present and in previous studies (Lee et al 2004;Keates et al 2005) are suitable models for Hp coculture/adhesion experiments.…”

Section: Discussionmentioning

confidence: 59%

Terminal α1,4-linked N-acetylglucosamine in Helicobacter pylori-associated Intestinal Metaplasia of the Human Stomach and Gastric Carcinoma Cell Lines

Ferreira

Marcos

David

et al. 2006

J Histochem Cytochem.

View full text Add to dashboard Cite

(JN) S U M M A R Y Helicobacter pylori (Hp) infection is associated with the development of gastric lesions including gastritis, intestinal metaplasia (IM), and gastric carcinoma. In humans, Hp is found almost exclusively in the foveolar epithelium of the gastric mucosa and rarely colonizes the deeper portions where mucous cells of the glands produce mucins with terminal a1, 4-GlcNAc O-glycans. This structure exerts antimicrobial activity against Hp. The development of IM in the stomach is characterized by Hp clearance from the metaplastic glands and by major alterations in the expression of mucins and mucin-carbohydrates. The present work evaluated whether terminal a1,4-GlcNAc and sialyl-Tn antigen are implicated in the process of Hp clearance from metaplastic glands by analyzing the expression of these antigens in different types of IM-complete (n512) and incomplete (n58)-and in gastric cell lines. Terminal a1,4-GlcNAc was not detected in IM except in a single foci of one case, indicating that this structure is not implicated in the clearance of Hp from IM, in contrast to what is observed in normal gastric mucosa. None of the gastric carcinoma cell lines studied showed terminal a1,4-GlcNAc, suggesting that they do not display a gastric gland mucous cell phenotype and therefore are useful models for in vitro Hp studies. Finally, sialyl-Tn antigen colocalizes with MUC2 mucin and is present in all cases of complete and incomplete IM, suggesting that either or both can be implicated in Hp clearance from IM. (J Histochem Cytochem 54:585-591, 2006)

show abstract

Section: Discussionmentioning

confidence: 59%

Terminal α1,4-linked N-acetylglucosamine in Helicobacter pylori-associated Intestinal Metaplasia of the Human Stomach and Gastric Carcinoma Cell Lines

Ferreira

Marcos

David

et al. 2006

J Histochem Cytochem.

View full text Add to dashboard Cite

show abstract

“…Japan, USA) [11]. In this study, samples were collected by the same team and RNA extraction and pathology diagnosis were performed by the same individuals, allowing for a comparison between areas that differed greatly in relation to gastric cancer incidence and H. pylori cagA genotype.…”

Section: Discussionmentioning

confidence: 99%

Differences in interleukin 8 expression in Helicobacter pylori–infected gastric mucosa tissues from patients in Bhutan and the Dominican Republic

et al. 2015

Self Cite

View full text Add to dashboard Cite

The outcomes of Helicobacter pylori infection vary geographically. H. pylori strains, disease presentation, and environments differ markedly in Bhutan and Dominican Republic. The aims were to compare the strains, histology and expression of interleukin (IL)-8 and IL-10 from gastric mucosa from the two countries. H. pylori status was assessed by the combination of rapid urease test, culture and histology. Histology was evaluated using the updated Sydney System and cytokines in gastric biopsies were measured using real-time PCR. There were 138 subjects from Bhutan and 155 from Dominican Republic. The prevalence of H. pylori infection was 65% and 59%, respectively. The genotype of cagA was predominantly East-Asian type in Bhutan vs. Western type in Dominican Republic. Gastritis severity was significantly higher in H. pylori-infected subjects from Bhutan than those from Dominican Republic. IL-8 expression by H. pylori-infection was 5.5-fold increase in Bhutan vs. 3-fold in Dominican Republic (p <0.001); IL-10 expression was similar. IL-8 expression levels among H. pylori-infected cases tended to be positively correlated with polymorphonuclear leucocyte (PMN) and monocyte infiltration (MNC) scores in both countries. IL-8 expression among those with grade 2 and 3 PMN and MNC was significantly higher in Bhutan than in Dominican Republic. The difference in IL-8 expression in two countries is reflected in the different disease pattern between them. Whether the dominant factor is differences in H. pylori virulence, in host-H. pylori-environmental interactions, genetic factors or all remains unclear. However, severity of inflammation appears to be a critical factor in disease pathogenesis. We compared IL-8 mRNA levels between high gastric cancer risk country; Bhutan (mainly East Asian type H. pylori) and lower gastric cancer risk country; Dominican Republic (mainly Western type H. pylori).

show abstract

“…Interestingly, 67.6% of East Asian H. pylori strains possessed RpoB Thr , compared to only 2.9% of western strains, and RpoB Thr induced significantly more IL-8 secretion from MKN45 cells than RpoB Ala . 55 This protein may therefore increase inflammation in infected subjects in East Asia and increase the risk of development of more severe gastroduodenal diseases.…”

Section: Blood Group Antigen Binding Adhesion a (Baba)mentioning

confidence: 99%

The inflammatory and immune response to Helicobacter pylori infection

Robinson

Argent

Atherton

2007

Best Practice & Research Clinical Gastroenterology

158

149

View full text Add to dashboard Cite

Alanine-Threonine Polymorphism of Helicobacter pylori RpoB Is Correlated with Differential Induction of Interleukin-8 in MKN45 Cells

Cited by 13 publications

References 35 publications

Terminal α1,4-linked N-acetylglucosamine in Helicobacter pylori-associated Intestinal Metaplasia of the Human Stomach and Gastric Carcinoma Cell Lines

Terminal α1,4-linked N-acetylglucosamine in Helicobacter pylori-associated Intestinal Metaplasia of the Human Stomach and Gastric Carcinoma Cell Lines

Differences in interleukin 8 expression in Helicobacter pylori–infected gastric mucosa tissues from patients in Bhutan and the Dominican Republic

The inflammatory and immune response to Helicobacter pylori infection

Contact Info

Product

Resources

About