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Cited by 22 publications
(22 citation statements)
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References 44 publications
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“…In this study, we demonstrate significant inhibition of FLG expression in keratinocyte cultures, organotypic skin cultures, and the skin of the animals exposed in vivo to PM2.5. This observation is consistent with previous reports (27,29). However, this is the first study that examined the effects of PM2.5 on skin both in vitro and in vivo.…”
Section: Discussionsupporting
confidence: 93%
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“…In this study, we demonstrate significant inhibition of FLG expression in keratinocyte cultures, organotypic skin cultures, and the skin of the animals exposed in vivo to PM2.5. This observation is consistent with previous reports (27,29). However, this is the first study that examined the effects of PM2.5 on skin both in vitro and in vivo.…”
Section: Discussionsupporting
confidence: 93%
“…In contrast, earlier studies examined the effects of PM in HaCat tumor keratinocyte cell line and human keratinocytes, using doses that far exceed those in the environment. We found that these PM2.5 doses were cytotoxic to primary keratinocytes in the current study (27)(28)(29).…”
Section: Discussionsupporting
confidence: 54%
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“…The cells from RHEs tissues were lysed, according to Hieda et al [41]. Protein concentrations were determined with the Pierce BCA protein assay kit (Thermo Fisher Scientific, USA).…”
Section: Protein Expression By Western Blottingmentioning
confidence: 99%
“…Total protein (40 µg) was subjected to electrophoresis in 8%, 12%, and 15% polyacrylamide gels under reducing conditions and transferred to polyvinylidene fluoride membranes (Hybond, Amersham Pharmacia Biotech, Piscataway, NJ, USA). The membranes were blocked in 3% BSA diluted in TBS-Tween 20 [41] for 1 h at room temperature and probed with the following antibodies: against cytokeratin 14 (Abcam-ab7800 1:1000), against cytokeratin 10 (Abcam ab76318 1:1000) and against involucrin (Abcam-ab27495 1:1000), and against β-actin (Abcam ab8227 1:2000) overnight at 4 • C. After washing, the membranes were incubated for 1 h at room temperature with the secondary antibody. Protein bands were detected by an enhanced chemiluminescence system (ECL, Amersham Pharmacia Biotech, Piscataway, NJ, USA).…”
Section: Protein Expression By Western Blottingmentioning
confidence: 99%