Agrobacterium Tumefaciens Mediated Transformation of rolC::Hd3a-GFP in Black Rice (Oryza Sativa L. cv. Cempo Ireng) to Promote Early Flowering

Purwestri, Yekti Asih; Sari, Resta Dewi Komala; Anggraeni, Lisa Novita; Sasongko, Aries Bagus

doi:10.1016/j.proche.2015.03.063

“…In order to promote early flowering in Cempo Ireng, insertion flowering gene Hd3a can be done by transformation rolC::Hd3a-GFP mediated by Agrobacterium tumefaciens into callus of the Cempo Ireng [34,35]. According to Tamaki et al [36], the expression of Hd3a in japonica rice sub-species was controlled by rolC promoter which accelerate the flowering period.…”

Section: Transgenic Lines Of Cempo Irengmentioning

confidence: 99%

Potential of Pigmented Rice Variety Cempo Ireng in Rice Breeding Program for Improving Food Sustainability

Dwiningsih¹,

Alkahtani²

2023

Preprint

2

1

0

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Cempo Ireng (Oryza sativa L. var. Cempo Ireng) is one of the black rice variety in Indonesia that consumed as functional food, not as a staple food because of the black rice contains anthocyanin pigment which has beneficial to human health. High concentration of anthocyanin reaching 393.93 ppm cause black color in the pericarp layer of Cempo Ireng which make this variety as an antioxidant source, low glycemic index, and free of gluten, leading to have higher price in the rice market. However, most of the farmers are not cultivate Cempo Ireng due to the longer vegetative phase up to 150 days compared to the white rice varieties which only takes 90 days, the taller phenotype of Cempo Ireng cause problems in cultivation, and also the purple color of Cempo Ireng attract birds and earhead bug (Leptocorisa oratorius) to feed on black rice caryopsis that cause severe yield loss. Transgenic methods, mutation technique, intensification systems, and physical treatment have been applied in Cempo Ireng to overcome those lack characteristics. In the future, Cempo Ireng also has a big potential to become a parent in cross breeding program because of the positive characters. The aim of this review is to describe the potential of Cempo Ireng in rice breeding program for improving food sustainability.

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“…In order to promote early fl owering in Cempo Ireng, insertion fl owering gene Hd3a can be done by transformation rolC:Hd3a-GFP mediated by Agrobacterium tumefaciens into callus of the Cempo Ireng [34,35]. According to Tamaki S, et al [36], the expression of Hd3a in japonica rice sub-species was controlled by rolC promoter which accelerate the fl owering period.…”

Section: Transgenic Lines Of Cempo Irengmentioning

confidence: 99%

Potential of Pigmented Rice Variety Cempo Ireng in Rice Breeding Program for Improving Food Sustainability

Dwiningsih¹,

Alkahtani²

2023

J Biomed Res Environ Sci

2

0

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Cempo Ireng (Oryza sativa L. var. Cempo Ireng) is one of the black rice variety in Indonesia that consumed as functional food, not as a staple food because of the black rice contains anthocyanin pigment which has beneficial to human health. High concentration of anthocyanin reaching 393.93 ppm cause black color in the pericarp layer of Cempo Ireng which make this variety as an antioxidant source, low glycemic index, and free of gluten, leading to have higher price in the rice market. However, most of the farmers are not cultivate Cempo Ireng due to the longer vegetative phase up to 150 days compared to the white rice varieties which only takes 90 days, the taller phenotype of Cempo Ireng cause problems in cultivation, and also the purple color of Cempo Ireng attract birds and earhead bug (Leptocorisa oratorius) to feed on black rice caryopsis that cause severe yield loss. Transgenic methods, mutation technique, intensification systems, and physical treatment have been applied in Cempo Ireng to overcome those lack characteristics. In the future, Cempo Ireng also has a big potential to become a parent in cross breeding program because of the positive characters. The aim of this review is to describe the potential of Cempo Ireng in rice breeding program for improving food sustainability.

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“…Thus, we should implement efforts to shorten this flowering period to enhance yield. To overcome this problem, a preliminary study has been initiated in order to investigate and understand flowering behaviour [53]. Recent studies suggest that 'Cempo Ireng' black rice grown under neutral day conditions has a flowering pathway conserved with a rice model grown under SD/LD condition.…”

Section: Black Rice For Functional Foodsmentioning

confidence: 99%

Black rice as a functional food in Indonesia

Pratiwi

¹

,

Purwestri²

2017

FFHD

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Background: There are many local black rice cultivars in Indonesia, yet only a few of these are formally described in the literature. It has been reported that black rice has many phytochemical variants which may contribute to its use as a functional food, including nutraceuticals and secondary metabolites such as anthocyanin, oryzanol, and more. The purpose of this article was to review literature describing black rice cultivars from Indonesia, with a particular focus on its potential use as a functional food. Our literature search revealed several articles that describe black rice in relation to its nutraceutical properties and its role in reducing non-communicable diseases. Other studies describe the diversity of local pigmented rice and its potential for lowering the risk of hyperlipidemia, hyperglycemia, and for cancer prevention. Black rice has been described as a functional food in several countries; however, there is great diversity among cultivars and further research on Indonesian varieties will determine whether local variants are candidates as well for the development of functional foods. Keywords: black rice cultivar, functional food, non-communicable diseases, nutraceutical, phytochemical

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Agrobacterium Tumefaciens Mediated Transformation of rolC::Hd3a-GFP in Black Rice (Oryza Sativa L. cv. Cempo Ireng) to Promote Early Flowering

Cited by 7 publications

References 6 publications

Potential of Pigmented Rice Variety Cempo Ireng in Rice Breeding Program for Improving Food Sustainability

Potential of Pigmented Rice Variety Cempo Ireng in Rice Breeding Program for Improving Food Sustainability

Potential of Pigmented Rice Variety Cempo Ireng in Rice Breeding Program for Improving Food Sustainability

Black rice as a functional food in Indonesia

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