Agrobacterium-mediated Transformation of Tomato (Lycopersicon esculentum var. Pusa Ruby) with Coat-protein Gene of Physalis Mottle Tymovirus

Vidya, Chinnasamy; Manoharan, Muthusamy; Kumar, C.T. Ranjit; Savtthri, H.S.; Sita, G. Lakshmi

doi:10.1016/s0176-1617(00)80279-5

Cited by 31 publications

(3 citation statements)

References 21 publications

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“…It is an indeterminate, early growing variety with medium-sized, round, lobed, and slightly ribbed fruits, suitable for both table and processing purposes ( Figure 1 ) ( ; accessed on 17 June 2022). In India, the majority of biotechnological studies involving transformation are performed on PR [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ]. Multiple functional annotation, phenotypic, and transcriptomic studies also use the PR genotype [ 26 , 27 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Reference Guided De Novo Genome Assembly of Transformation Pliable Solanum lycopersicum cv. Pusa Ruby

Vats

Kumar

Mandlik

et al. 2023

Genes

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Solanum lycopersicum cv. Pusa Ruby (PR) is a superior tomato cultivar routinely used as a model tomato variety. Here, we report a reference-guided genome assembly for PR, covering 97.6% of the total single-copy genes in the solanales order. The PR genome contains 34,075 genes and 423,288 variants, out of which 127,131 are intragenic and 1232 are of high impact. The assembly was packaged according to PanSol guidelines (N50 = 60,396,827) with the largest scaffold measuring 85 megabases. The similarity of the PR genome assembly to Heinz1706, M82, and Fla.8924 was measured and the results suggest PR has the lowest affinity towards the hybrid Fla.8924. We then analyzed the regeneration efficiency of PR in comparison to another variety, Pusa Early Dwarf (PED). PR was found to have a high regeneration rate (45.51%) and therefore, we performed allele mining for genes associated with regeneration and found that only AGAMOUS-LIKE15 has a null mutation. Further, allele mining for fruit quality-related genes was also executed. The PR genome has an Ovate mutation leading to round fruit shape, causing economically undesirable fruit cracking. This genomic data can be potentially used for large scale crop improvement programs as well as functional annotation studies.

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Section: Introductionmentioning

confidence: 99%

Reference Guided De Novo Genome Assembly of Transformation Pliable Solanum lycopersicum cv. Pusa Ruby

Vats

Kumar

Mandlik

et al. 2023

Genes

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“…Various factors involve in efficient Agrobacterium transformation such as, Arobacterium cell density (Murray et al, 1998), regeneration and co-cultivation conditions (Hu and Phillips, 2001) and cell competence after wounding (Murray et al, 1998). McCormick, et al (1986) first reported on tomato transformation and ever since, numerous publications on transformation of various tomato cultivars (Sree Vidya et al, 2000;Hu and Phillips, 2001;Park et al, 2003;Raj et al, 2005;Sun et al, 2006 andSaker et al, 2007). Development of highly regenerative transformation protocol is essential in Agrobacterium transformation.…”

Section: Introductionmentioning

confidence: 99%

Development of Transgenic Tomato Expressing Antimicrobial Peptide Gene through Agrobacterium-Mediated Transformation

S¹

2015

IJIRSET

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Agrobacterium-mediated transformation was developed for tomato plant (cv. Arka vikas) using Antimicrobial peptide gene under the control of CaMV 35S promoter in pCAMBIA 2301 vector. Protocol was optimised by various parameters influencing transformation. Hypocotyledonary explants where treated with Agrobacterium 0.4 O.D 600 for 2 min and selected on kanamycin 100 mg l -1 . 59 shoots regenerated initially on MS media containing BAP -2.0 mg l -1 and TDZ -1.0 mg l -1 . 13 plants were finally survived on hardening and screened for the presence of gene by PCR. 360 bp of gene amplification confirmed the presence of gene in 8 plants. Transformation frequency of 11 % was observed.

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“…The explants were co-cultivated with 48 hr-grown Agrobacterium culture (OD 600 = 0.4) (with and without construct) and incubated for two days in the dark at 25°C (4). After co-cultivation, tomato explants were washed gently by half strength MS medium (17) and air dried on sterile filter paper. The explants were transferred to selection callusing medium containing augumentin (0.5 mg/ml) and kanamycin (0.1 mg/ml) and incubated at 25°C in 16hrs photoperiod.…”

mentioning

confidence: 99%

Nucleocapsid protein gene mediated resistance against groundnut bud necrosis virus in tomato using sense and antisense constructs

Raja¹,

Rk²

2011

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Agrobacterium-mediated Transformation of Tomato (Lycopersicon esculentum var. Pusa Ruby) with Coat-protein Gene of Physalis Mottle Tymovirus

Cited by 31 publications

References 21 publications

Reference Guided De Novo Genome Assembly of Transformation Pliable Solanum lycopersicum cv. Pusa Ruby

Reference Guided De Novo Genome Assembly of Transformation Pliable Solanum lycopersicum cv. Pusa Ruby

Development of Transgenic Tomato Expressing Antimicrobial Peptide Gene through Agrobacterium-Mediated Transformation

Nucleocapsid protein gene mediated resistance against groundnut bud necrosis virus in tomato using sense and antisense constructs

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