Agrobacterium-mediated transformation of Solanum tuberosum L. cv. ?Russet Burbank?

Newell, C. A.; Rozman, Renee J.; Hinchee, Maud A. W.; Lawson, E. C.; Haley, Lisa; Sanders, Patricia R.; Kaniewski, Wojciech; Tumer, Nilgun E.; Horsch, Robert B.; Fraley, Robert T.

doi:10.1007/bf00233028

Cited by 70 publications

(35 citation statements)

References 22 publications

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“…A plant expression vector incorporating this gene was cloned into the T-DNA segment between the right-and left-border region and introduced into potato via Agrobacterium tumefaciensmediated transfer [22]. The A. tumefaciens ABI strain used to deliver this plasmid (pMON10547) contained the disarmed (lacking the T-DNA phytohormone genes) pTiC58 plasmid pMP90RK [16] in the chloramphenicol-resistant derivative ofA.…”

Section: Resultsmentioning

confidence: 99%

Genetically improved potatoes: protection from damage by Colorado potato beetles

et al. 1993

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Russet Burbank potato plants have been genetically improved to resist insect attack and damage by Colorado potato beetles (Leptinotarsa decemlineata (Say)) by the insertion of a cryIIIA gene encoding the insect control protein of Bacillus thuringiensis var. tenebrionis. A modified gene that dramatically improved plant expression of this protein was utilized. Its expression in Russet Burbank potato plants resulted in protection from damage by all insect stages in the laboratory and in dramatic levels of protection at multiple field locations. Analysis of these genetically modified potatoes indicated that they conform to the standards for Russet Burbank potatoes in terms of agronomic and quality characteristics including taste.

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Section: Resultsmentioning

confidence: 99%

Genetically improved potatoes: protection from damage by Colorado potato beetles

et al. 1993

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“…Samsun and Nicotiana benthamiana) by the leaf disc method (13) and potato (Solanum tuberosum cv. Russet Burbank) by transformation of stem sections (14). Vacuum Infiltration.…”

Section: Methodsmentioning

confidence: 99%

Broad-spectrum virus resistance in transgenic plants expressing pokeweed antiviral protein.

Lodge

Kaniewski

Tumer

1993

Proc. Natl. Acad. Sci. U.S.A.

257

165

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Exogenous application of pokeweed antiviral protein (PAP), a ribosome-inhibiting protein found in the cell walls of Phytolacca americana (pokeweed), protects heterologous plants from viral infection. A cDNA clone for PAP was isolated and introduced into tobacco and potato plants by transformation with Agrobacterium tumefaciens. Transgenic plants that expressed either PAP or a double mutant derivative of PAP showed resistance to infection by different viruses. Resistance was effective against both mechanical and aphid transmission. Analysis of the vacuum infiltrate of leaves expressing PAP showed that it is enriched in the intercellular fluid. Analysis of resistance in transgenic plants suggests that PAP confers viral resistance by inhibiting an early event in infection. Previous methods for creating virus-resistant plants have been specific for a particular virus or closely related viruses. To protect plants against more than one virus, multiple genes must be introduced and expressed in a single transgenic lne. Expression of PAP in transgenic plants offers the possibility of developing resistance to a broad spectrum of plant viruses by expression of a single gene.

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“…Porém, quando cultivados na ausência de can, esses explantes diferenciaram parte aé-rea (Figura 3). A utilização de can como agente seletivo também foi observada por Newell et al (1991) que transformaram entrenós da cv. Russet Burbank com o gene da capa protéica do vírus X e Y. Os explantes produziram brotações com uma freqüência de 60% na ausên-cia de seleção e de 10% em meio com 100 mg.L -1 de can.…”

Section: Resultsunclassified

Transformação genética da batata cultivar Achat via Agrobacterium tumefaciens

et al. 2000

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O melhoramento genético tradicional da batata (Solanum tuberosum L.) é lento e defronta-se com uma gama de dificuldades. A espécie cultivada é tetraplóide com segregação polissômica, e muitas das cultivares comerciais apresentam baixa fertilidade de pólen ou não florescem (Vayda et al., 1992). Nesse cenário, a utilização das técnicas de transformação genética em batata teria a vantagem de possibilitar a incorporação, em clones considerados elites, de genes específicos que codificam características de interesse. Achat é uma das cultivares lideres no mercado nacional, no entanto, reduções nos níveis de produtividade desse genótipo têm sido reportados devido a infecção por viroses do grupo potivirus e luteovirus.Estratégias de engenharia genética usando genes derivados de patógenos têm sido empregadas com sucesso no controle de doenças virais em diversas culturas incluindo a batata (Scholthof et al., 1993), permitindo o desenvolvimento, a curto prazo, de cultivares melhora- ABSTRACT Genetic transformation of potato cultivar Achat by Agrobacterium tumefaciens.Nodal segment explants of potato (Solanum tuberosum L.) cv Achat were excised from in vitro growing plants, and transformed with Agrobacterium tumefaciens LBA4404 carrying the binary vector pBI121 with npt II and gus-intron genes. The regeneration of putative transformants was done in medium supplemented with 50 mg.L -1 of kanamycin. Putative transformants expressing kanamycin resistance and b-glucuronidase activity were identified. PCR analysis of these plants showed amplification of the npt II in gus + plants. Southern blotting hybridization revealed the insertion of at least one copy of the npt II gene.

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Agrobacterium-mediated transformation of Solanum tuberosum L. cv. ?Russet Burbank?

Cited by 70 publications

References 22 publications

Genetically improved potatoes: protection from damage by Colorado potato beetles

Genetically improved potatoes: protection from damage by Colorado potato beetles

Broad-spectrum virus resistance in transgenic plants expressing pokeweed antiviral protein.

Transformação genética da batata cultivar Achat via Agrobacterium tumefaciens

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