Agrobacterium-mediated transformation of leaf base derived callus tissues of popular indica rice (Oryza sativa L. sub sp. indica cv. ADT 43)

Karthikeyan, Alagarsamy; Pandian, Shunmugiah Karutha; Ramesh, Manikandan

doi:10.1016/j.plantsci.2011.05.011

Cited by 41 publications

(27 citation statements)

References 86 publications

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“…However, the use of such technologies for the successful transformation of non-embryogenic cultures in general, and of grapevine and gymnosperms in particular, is limited [41,56,62,63]. We report herein the successful stable transformation of Vitis vinifera cv.…”

Section: Discussionmentioning

confidence: 99%

A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

et al. 2015

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One of the major intent of metabolic engineering in cell culture systems is to increase yields of secondary metabolites. Efficient transformation methods are a priority to successfully apply metabolic engineering to cell cultures of plants that produce bioactive or therapeutic compounds, such as Vitis vinifera and Taxus x media. The aim of this study was to establish a reliable method to transform non-embryogenic cell cultures of these species. The V. vinifera cv. Gamay/cv. Monastrell cell lines and Taxus x media were used for Agrobacterium-mediated transformation using the Gateway-compatible Agrobacterium sp. binary vector system for fast reliable DNA cloning. The Taxus x media and Vitis cell lines were maintained in culture for more than 4 and 15 months, respectively, with no loss of reporter gene expression or antibiotic resistance. The introduced genes had no discernible effect on cell growth, or led to extracellular accumulation of phytoalexin trans-Resveratrol (t-R) in response to elicitation with methylated cyclodextrins (MBCD) and methyl jasmonate (MeJA) in the grapevine transgenic cell lines compared to the parental control. The method described herein provides an excellent tool to exploit exponentially growing genomic resources to enhance, optimize or diversify the production of bioactive compounds generated by grapevine and yew cell cultures, and offers a better understanding of many grapevine and yew biology areas.

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Section: Discussionmentioning

confidence: 99%

A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

et al. 2015

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“…Cocultivation for more than 2 days not only resulted in browning of calli but also led to repeated Agrobacterium infection, which ultimately resulted in the loss of regeneration potential (Karthikeyan et al, 2011). For cocultivation of explants of recalcitrant crops with Agrobacterium, the vir gene inducer AS was employed (Veluthambi et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Ploidy stability of Oryza sativa. L cv IR64 transformed with the moth bean P5CS gene with significant tolerance against drought and salinity

Priya¹,

Krishnan²,

Ramesh³

2015

Turk J Biol

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Rice is one of the world's most important crops. Thus, it is necessary to improve it by genetic transformation. IR64 is a highyielding and disease-resistant variety, but it is sensitive to abiotic stresses, mainly salinity and drought. Therefore, an efficient transformation system is a prerequisite for transforming this indica rice with a stress-tolerant gene, the potent osmolyte proline-coding P5CS gene of Vigna aconitifolia. Successful transgene integration was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. A Mendelian pattern of transgene inheritance was also observed in the progeny plants. The individual transformants expressed significant tolerance over wild-type control plants, as exhibited by NaCl tolerance, lipid peroxidation levels, and total chlorophyll content. Percentage of germination, height, and weight of the plantlet at 400 mM mannitol stress was 60%, 8.1 cm, and 92.5 mg, respectively. Total chlorophyll retention and malondialdehyde content at 200 mM NaCl stress was 65.3% and 8.2 n mol g -1 FW, respectively. These results revealed the abiotic stress tolerance conferred by Δ1-pyrroline-5-carboxylate synthetase. Additionally, flow cytometry analysis confirmed the absence of somaclonal variation. Hence, an efficient genetic transformation can be accomplished in other indica rice cultivars by using this protocol.

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“…Saika and Toki (2010) observed that calli of the indica cultivar Kasalath were transformed 10 times as efficiently as was the japonica cultivar Nipponbare. Karthikeyan et al (2011) found that callus induced from the leaf bases of germinating seeds was good for transformation of indica cultivar ADT 43. Sahoo et al (2011) optimized transformation protocols for seed-derived callus of four indica cultivars.…”

Section: Progress In Rice Transformationmentioning

confidence: 99%

Progress of cereal transformation technology mediated by Agrobacterium tumefaciens

2014

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Monocotyledonous plants were believed to be not transformable by the soil bacterium Agrobacterium tumefaciens until two decades ago, although convenient protocols for infection of leaf disks and subsequent regeneration of transgenic plants had been well established in a number of dicotyledonous species by then. This belief was reinforced by the fact that monocotyledons are mostly outside the host range of crown gall disease caused by the bacterium and by the failures in trials in monocotyledons to mimic the transformation protocols for dicotyledons. However, a key reason for the failure could have been the lack of active cell divisions at the wound sites in monocotyledons. The complexity and narrow optimal windows of critical factors, such as genotypes of plants, conditions of the plants from which explants are prepared, tissue culture methods and culture media, pre-treatments of explants, strains of A. tumefaciens, inducers of virulence genes, transformation vectors, selection marker genes and selective agents, kept technical hurdles high. Eventually it was demonstrated that rice and maize could be transformed by co-cultivating cells of callus cultures or immature embryos, which are actively dividing or about to divide, with A. tumefaciens. Subsequently, these initial difficulties were resolved one by one by many research groups, and the major cereals are now transformed quite efficiently. As many as 15 independent transgenic events may be regenerated from a single piece of immature embryo of rice. Maize transformation protocols are well established, and almost all transgenic events deregulated for commercialization after 2003 were generated by Agrobacterium-mediated transformation. Wheat, barley, and sorghum are also among those plants that can be efficiently transformed by A. tumefaciens.

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Agrobacterium-mediated transformation of leaf base derived callus tissues of popular indica rice (Oryza sativa L. sub sp. indica cv. ADT 43)

Cited by 41 publications

References 86 publications

A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

Ploidy stability of Oryza sativa. L cv IR64 transformed with the moth bean P5CS gene with significant tolerance against drought and salinity

Progress of cereal transformation technology mediated by Agrobacterium tumefaciens

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