Agrobacterium-mediated genetic transformation of grapevine (Vitis vinifera L.) with a novel stilbene synthase gene from Chinese wild Vitis pseudoreticulata

Fan, Chaohong; Pu, Ni; Wang, Xiping; Wang, Yuejin; Li, Fang; Xu, Weirong; Zhang, Jianxia

doi:10.1007/s11240-007-9324-2

Cited by 68 publications

(50 citation statements)

References 36 publications

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“…Some of these compounds also possess promising therapeutic activity in various cancers, including breast, prostate, and neuroblastoma (Aggarwal et al 2004;Laux and Aregullin 2004;King et al 2005;Baur and Sinclair 2006). Direct evidence of resveratrol's biological activity (Adrian et al 1997) and a clear relationship between its production and disease resistance have been demonstrated using transgenic tobacco (Hain et al 1993), oil-seed (Thomzik 1993), tomato Giovinazzo et al 2005;Nicoletti et al 2007), rice (Stark-Lorenzen et al 1997), wheat (Leckband and Lörz 1998), kiwi (Kobayashi et al 2000), alfalfa (Hipskind and Paiva 2000), apple (Szankowski et al 2003), papaya (Zhu et al 2004), banana (Vishnevetsky et al 2005), pea (Richter et al 2006), hop (Schwekendiek et al 2007) and grapevine (Fan et al 2008). On the other hand, little is known about cis-acting elements and transcription factors controlling expression of stilbene synthase in response to various environmental stimuli.…”

Section: Introductionmentioning

confidence: 99%

“…VpSTS-overexpressing V. vinifera cv. Thompson seedless, under control of the highly active cauliXower mosaic virus 35S promoter were shown to have a resveratrol concentration 5.5 times higher than that of non-transformed control plants (Fan et al 2008). This prompted us to investigate the novel regulatory mechanism of STS in Chinese wild Vitis.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata

Ding

et al. 2009

Planta

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126

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Stilbene synthase is a plant-specific polyketide synthase, and plays important roles in diverse metabolic processes. The genomic stilbene synthase gene was cloned from accession "Baihe-35-1" of Chinese wild Vitis pseudoreticulata, and a stilbene synthase of V. pseudoreticulata (VpSTS) transcripts expressed in the grape-powdery mildew interaction were determined by semi-quantitative RT-PCR. To monitor VpSTS expression in plant, the promoter region flanking the 5' VpSTS coding region was isolated from the genomic DNA of Chinese wild V. pseudoreticulata accession Baihe-35-1. Alignment of the VpSTS promoter sequence showed a 56.4% identity to Vitis vinifera. To identify the upstream region of the VpSTS gene required for promoter activity, a series of VpSTS promoter deletion derivatives was constructed. Each deletion construct was analyzed by Agrobacterium-mediated transient transformation in grapevine and tobacco leaves after infection by Uncinula necator and Alternaria alternata. In transiently transformed grapevine leaves, GUS activity was also determined after treatment with salicylic acid (SA) and 4 degrees C cold. Analysis of a series of 5' deletions of the VpSTS promoter in grapevine leaves indicated that the proximal 162 bp from the transcription initiation site was proved to be necessary for establishing both the constitutive and induced pattern of expression.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata

Ding

et al. 2009

Planta

Self Cite

126

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“…To construct the expression vector of VpROMT, the expression vector pWR306 derived from pCAMBIA1303 and pSB166 as previously described by Fan et al (2008) was used. The VpROMT gene cloned from V. pseudoreticulata driven by enhanced cauliflower mosaic virus 35S promoter (ED35S) fused after a 5′-untranslated leader sequence from tobacco mosaic virus Omega (designated as ED35S/X) was inserted into pWR306.…”

Section: Construction Of Pvpromt::gus Fusion Vectormentioning

confidence: 99%

“…We previously reported the gene encoding STS in transgenic Thompson seedless (Fan et al 2008) and recently characterized the novel STS promoter from the highly powdery mildew (PM)-resistant Chinese wild Vitis pseudoreticulata (Xu et al 2010). Here, we isolated the VpROMT gene from Chinese wild V. pseudoreticulata, which had 98.9% and 98.3% identity to the resveratrol O-methyltransferase gene of V. vinifera at the nucleotide and amino acid levels.…”

Section: Introductionmentioning

confidence: 99%

Co-expression of VpROMT gene from Chinese wild Vitis pseudoreticulata with VpSTS in tobacco plants and its effects on the accumulation of pterostilbene

Zhao

et al. 2011

Protoplasma

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Plant secondary metabolites, such as stilbenes, have fungicidal potential and have been found in several plant species. Stilbenes in grapevine, such as resveratrol and pterostilbene, have recently attracted much attention, they are not only helping the plant to fight against pathogen attack, but they are also being widely used as ingredients of fungicide, anti-inflammatory drugs, antioxidant, and anti-infective agents. However, resveratrol O-methyltransferase gene, related with the synthesis of pterostilbene from resveratrol, has not been characterized effectively from Chinese wild Vitis pseudoreticulata. In this study, a candidate of resveratrol O-methyltransferase gene designated as VpROMT was isolated from a powdery mildew-resistant Chinese wild V. pseudoreticulata 'Baihe-35-1', and characterization studies were performed. Expression studies showed that VpROMT was predominantly expressed in developing roots yet not found in the leaves, stems, nor tendrils when the plants are not challenged. Results of qRT-PCR showed that VpROMT was rapidly induced by Erysiphe necator in V. pseudoreticulata and by methyl-jasmonate, UV-irradiation in suspension culture cells of Vitis romanetii. The expression level varies in different tissues of grapevine, which MeJA and UV-C treatment significantly upregulated the expression of VpROMT gene while UV-B treatment failed to. Co-expression of VpROMT and grapevine stilbene synthase (VpSTS) gene leads to the accumulation of pterostilbene in leaves of tobacco (Nicotiana tabacum) indicating that VpROMT was able to catalyze the biosynthesis of pterostilbene from resveratrol in over-expression transgenic tobacco plants.

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“…Stilbene synthase (STS) or resveratrol synthase condenses three molecules of malonylCoA and one molecule of cumaryl-CoA to form t-resveratrol (Rupprich et al 1980;Xu et al 2010). STS exists as a multigene family in most stilbenoid-producing species, including grapes, peanuts, and pines (Sparvoli et al 1994;Fan et al 2008;Chong et al 2009). Southern blot experiments have suggested that the genome of Vitis vinifera contains 15-20 STS genes (Sparvoli et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Salicylic acid induces alterations in the methylation pattern of the VaSTS1, VaSTS2, and VaSTS10 genes in Vitis amurensis Rupr. cell cultures

2014

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Salicylic acid (SA) treatment selectively reduced the cytosine DNA methylation of stilbene synthase ( STS ) genes and stimulated resveratrol production in cell cultures of Vitis amurensis. The effect of salicylic acid (SA) on plant growth, flowering time, and fruit number is known to correlate with the level of DNA methylation, while the potential correlation between SA-induced changes in DNA methylation and biosynthesis of secondary metabolites has not been studied. Trans-resveratrol, a naturally occurring plant phenol, has been reported to exhibit a wide range of valuable biological and pharmacological properties. In this study, cell cultures of Vitis amurensis capable of producing t-resveratrol were used as a model system to study whether the SA-induced increase in t-resveratrol production is associated with changes in DNA methylation of stilbene synthase (STS) genes. T-resveratrol is synthesized via the phenylpropanoid pathway, in which STS genes are the key enzymes. Treatment of V. amurensis callus cultures with SA significantly increased t-resveratrol production and the expression of certain STS genes (e.g., VaSTS2 and VaSTS10). A marked decrease in the methylation of the VaSTS2 and VaSTS10 genes in response to SA was demonstrated using bisulfite sequencing, while no considerable changes were detected in the methylation of VaSTS1, a constitutively and highly expressed STS gene. The obtained results show that SA treatment selectively reduced cytosine methylation of VaSTS genes. The data suggest that selective DNA demethylation of particular STS genes could be necessary for the activation of t-resveratrol biosynthesis in response to SA. This finding provides an insight into the mechanism of SA action and biosynthesis of secondary metabolites in plant cells.

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Agrobacterium-mediated genetic transformation of grapevine (Vitis vinifera L.) with a novel stilbene synthase gene from Chinese wild Vitis pseudoreticulata

Cited by 68 publications

References 36 publications

Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata

Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata

Co-expression of VpROMT gene from Chinese wild Vitis pseudoreticulata with VpSTS in tobacco plants and its effects on the accumulation of pterostilbene

Salicylic acid induces alterations in the methylation pattern of the VaSTS1, VaSTS2, and VaSTS10 genes in Vitis amurensis Rupr. cell cultures

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