Aging peach palm (Bactris gasipaes Kunth) cultures lose embryogenic potential and metabolic cellular function due to continuous culture in hypoxic environments

Ree, Joseph Francis; Polesi, Luiza Giacomolli; Back, Franklin Panato; Bertolazi, Amanda Azevedo; Silveira, Vanildo; Guerra, Miguel Pedro

doi:10.1007/s11240-019-01710-7

Cited by 16 publications

(9 citation statements)

References 110 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transverse TCLs were placed with the basal cut in contact with the culture medium composed of the Murashige and Skoog (1962) salts, supplemented with Morel and Wetmore (1951) vitamins, sucrose (30 g L –1 ), glutamine (0.5 g L –1 ), Phytagel ™ (2 g l –1 ) (Sigma-Aldrich, St. Louis, MO, USA), activated charcoal (1.5 g L –1 ) ( Steinmacher et al, 2007c ; Heringer et al, 2014 ; Ree et al, 2019 ), and contained in 9-cm Petri dishes (ca. 25 ml of medium in each).…”

Section: Methodsmentioning

confidence: 99%

“…Explants that developed somatic embryos were transferred to the "Development" medium, composed of the basal medium but with a lower picloram concentration (10 µM), contained 10.3389/fpls.2022.995307 in 9-cm Petri dishes and under dark conditions at 23-24 • C. After 2 months, cultures with mature somatic embryos were transferred to the "Conversion" culture medium, in which picloram was replaced by abscisic acid (ABA) (5 µM), also contained in 9-cm Petri dishes and under the same conditions described above. Finally, after two additional weeks, these explants were transferred to the culture medium without plant growth regulators in glass flasks (5.5 cm in diameter and 7 cm in height) and under light conditions (12 h photoperiod, 19 µmol m −2 s −1 ) and a temperature of 23-24 • C, to form plantlets (Steinmacher et al, 2007c;Heringer et al, 2014;Ree et al, 2019).…”

Section: Culture and Evaluationmentioning

confidence: 99%

See 1 more Smart Citation

Somatic embryogenesis and plant regeneration from transverse thin cell layers of adult peach palm (Bactris gasipaes) lateral offshoots

et al. 2022

View full text Add to dashboard Cite

In this work, we report a successful protocol to obtain in vitro peach palm (Bactris gasipaes Kunth) “Diamantes 10” plants through somatic embryogenesis from transverse thin cell layer (TCL) explants, dissected from three sections (basal, medial, and apical) of lateral offshoots of adult plants cultured on different concentrations of 4-amino-3,5,6-trichloropicolonic acid (picloram). After swelling and development of primary callus in all treatments, without any strong effect of explant origin or picloram concentration, it was possible to observe the formation of embryogenic structures and the exact point from where they developed. Browning was also observed and correlated to the induction treatments, although it was not an impairment for the production of embryogenic structures. Subsequent maturation and conversion of somatic embryos into plantlets allowed their acclimatization 17 months after culture initiation (ACI), which was quicker than previous reports with juvenile tissues (from embryos or seed-germinated plantlets). To the best of our knowledge, this is the first report on peach palm regeneration through somatic embryogenesis from TCL explants from adult plants and could constitute, after fine-tuning the acclimatization stage, a tool for mass clonal propagation of elite genotypes of this open-pollinated crop, as well as for the establishment of conservation strategies of in situ gene bank plant accessions endangered due to aging and other threats.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Culture and Evaluationmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration from transverse thin cell layers of adult peach palm (Bactris gasipaes) lateral offshoots

et al. 2022

View full text Add to dashboard Cite

show abstract

“…However, reports indicate that in pine species, the situation is quite different. It has been observed that the yields in maturation and the length of the Se decrease as a function of the number (and time) of subcultures [16][17][18][19][20]. In addition, morphological degradation prevents the formation of early embryos, indicating a global loss of MA, which may be increased by manipulation during subculture [21].…”

Section: Introductionmentioning

confidence: 99%

Effects of Long-Term Subculture on Maturation Ability and Plant Conversion in Pinus radiata: Using FT-IR Spectroscopy to Determine Biomarkers of Embryogenic Tissue Aging

Lineros

Rojas-Rioseco

Hernández

et al. 2023

Forests

View full text Add to dashboard Cite

The forestry industry has integrated somatic embryogenesis into its clonal programs due to the generation of a high number of plants from selected genotypes at low cost. Somatic embryos are generated in a stressful environment after multiplication of the proembryogenic masses; thus, it is critical to determine the degree of stability of the embryogenic cultures and their potential for mass propagation. Maturation ability in cultures of different ages was evaluated in conjunction with the integrity of the proembryogenic masses, germination rate, hypocotyl and root length, plant conversion, and ex vitro survival. To identify differences in embryogenic tissue from different subcultures, their DNA was analyzed using FT-IR spectroscopy. A significant decrease in the production of somatic embryos was detected from week 15, and some lines even stopped producing embryos. Germination rate, hypocotyl length, and plant conversion were negatively affected by long-term cultivation, while root length and ex vitro survival were not significantly affected. The results obtained from the FT-IR spectroscopy analysis indicate that it is feasible to use mid-infrared spectroscopy to differentiate between embryogenic tissues with different cumulative subculture times based on the spectra obtained from their DNA, which is directly related to maturation ability.

show abstract

“…Somatic embryogenesis is the production of thousands of identical plants from a single explant (Ree et al 2020), which can be any part of the plant. However, this technique remains challenging for many species, particularly E. edulis.…”

Section: Introductionmentioning

confidence: 99%

Cell reprogramming via direct somatic embryogenesis in an Atlantic Forest species vulnerable to extinction: polarity of Euterpe edulis stem segments induced with pyridinecarboxylic acid

Mello

Correia

Hegedus

et al. 2023

Preprint

View full text Add to dashboard Cite

Euterpe edulis Martius, commonly known as juçara, has high economic value because its palm heart is considered a delicacy and its fruit, which is rich in antioxidants, is considered a “super fruit.” Because this endangered species can only be propagated via the seminiferous route, we aimed to analyze somatic embryogenesis of stem explants from E. edulis seedlings in response to their polarity and the type and concentration of growth regulators. Immature seeds were collected from a selected matrix in Pedra Menina (ES/MG, Brazil) and germinated in vitro. Six-month-old seedlings were segmented into four explants based on their polarity, and placed in culture medium supplemented with 50, 100, 150, 200, 250, 300, 450 or 600 µM picloram (PIC). After induction, the explants were transferred to maturation medium supplemented with 1-naphthaleneacetic acid (0.53 µM) and 2-isopentenyladenine (12.3 µM) and two maturation times (30 and 60 days) were evaluated. After 60 days of induction, proembryos appeared asynchronously directly from the stem segments. Upon transfer to maturation medium, a large number of somatic embryos and masses were observed at both times. The polarity of the explants did not influence their embryogenic induction, and all four stem segments could be used for somatic embryogenesis following treatment with 150 µM PIC. A large number of somatic embryos were generated during later stages of maturation. It is recommended to remove the explants from the maturation medium after 30 days to avoid oxidation.

show abstract

Aging peach palm (Bactris gasipaes Kunth) cultures lose embryogenic potential and metabolic cellular function due to continuous culture in hypoxic environments

Cited by 16 publications

References 110 publications

Somatic embryogenesis and plant regeneration from transverse thin cell layers of adult peach palm (Bactris gasipaes) lateral offshoots

Somatic embryogenesis and plant regeneration from transverse thin cell layers of adult peach palm (Bactris gasipaes) lateral offshoots

Effects of Long-Term Subculture on Maturation Ability and Plant Conversion in Pinus radiata: Using FT-IR Spectroscopy to Determine Biomarkers of Embryogenic Tissue Aging

Cell reprogramming via direct somatic embryogenesis in an Atlantic Forest species vulnerable to extinction: polarity of Euterpe edulis stem segments induced with pyridinecarboxylic acid

Contact Info

Product

Resources

About