Aggregation Pheromone of &lt;I&gt;Metamasius spinolae&lt;/I&gt; (Coleoptera: Curculionidae): Chemical Analysis and Field Test

Tafoya, Felipe; Whalon, Mark E.; Vandervoot, Christine; Coombs, Andrea B.; Cibrián‐Tovar, Juan

doi:10.1603/0046-225x(2007)36[53:apomsc]2.0.co;2

Cited by 15 publications

(7 citation statements)

References 11 publications

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“…The major compound (4) could reach 80% of the blend. The corresponding EAD‐active compounds were identified by GC‐MS as (1) 2‐methyl‐4‐heptanol [GC‐EI‐MS m‐z 112 (M + ‐18, 1%), 97 (1), 87 (38), 73 (68), 69 (70), 55 (100), 43 (90)] (mass spectrum match with the 2‐methyl‐4‐heptanol reported as pheromone component of Metamasius hemipterus L.; Ramirez‐Lucas et al., 1996); (2) 2‐methyl‐4‐octanol [GC‐EI‐MS m‐z 126 (M + ‐18, 1%), 111 (1), 87 (38), 69 (100), 57 (14), 43 (53)] (mass spectrum match with 2‐methyl‐4‐octanol reported as pheromone component of Sphenophorus levis Vaurie ; Zarbin et al., 2003); (3) 2‐methyl‐4‐heptanone [GC‐EI‐MS m‐z 128 (M + , 25%) (113 (M + ‐15, 10), 85 (75), 71 (73), 57 (100), 43 (75)] (mass spectrum match with 2‐methyl‐4‐heptanone reported as pheromone component of Metamasius spinolae Gyllenhal ; Tafoya et al., 2007); and (4) 2‐methyl‐4‐octanone [GC‐EI‐MS m‐z 142(M + , 15%), 127 (M + ‐15, 5), 100 (10), 85 (75), 57 (100), 43 (25)] (mass spectrum match with 2‐methyl‐4‐octanone produced by M. hemipterus sericus ; Perez et al., 1997). When 10 ng of synthetic compounds were subjected to GC‐EAD analysis, repeatable EAD responses were evoked by all four compounds (Figure 1B).…”

Section: Resultsmentioning

confidence: 99%

Aggregation pheromone of the agave weevil, Scyphophorus acupunctatus

Ruiz-Montiel¹,

García‐Coapio²,

Rojas³

et al. 2008

Entomologia Exp Applicata

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The agave weevil, Scyphophorus acupunctatus Gyllenhal (Coleoptera: Curculionidae), is the most important insect pest of wild and cultivated agaves in the world. Combined gas chromatography‐electroantennography (GC‐EAD) analysis of male volatile extracts showed that four peaks elicited antennal responses from males and females. The peaks were identified by GC‐mass spectrometry (GC‐MS) as 2‐methyl‐4‐heptanol (1), 2‐methyl‐4‐octanol (2), 2‐methyl‐4‐heptanone (3), and 2‐methyl‐4‐octanone (4). Electroantennogram (EAG) recordings of both sexes to 0.01‐, 0.1‐, 1‐, and 10‐µg stimulus load of synthetic compounds showed that the dose of the tested compounds and weevil sex significantly influenced the antennal response of S. acupunctatus. However, there was no sexual dimorphism in the antennal responses to the four synthetic compounds evaluated because the EAG profiles revealed no interaction between doses by sex. Antennae of S. acupunctatus were most sensitive to compounds 2 and 4, reaching the threshold at a 0.01‐µg stimulus load. Weevil antennae were less sensitive to compounds 1 and 3, and the threshold response to these compounds was 0.1 µg. Behavioural evaluation of the synthetic compounds showed them to be attractive to both males and females in a Y‐tube olfactometer. Field experiments confirmed the laboratory results, showing that all components, singly or in blends, were attractive to the weevils. In general, traps baited with the quaternary blend of compounds 1–4 captured significantly more weevils than traps baited with males. However, compounds 3 and 4 were sufficient to obtain captures equivalent to those by the quaternary blend. The potential use of the aggregation pheromone in the development of a mass‐trapping programme as a viable pest management alternative for S. acupunctatus is discussed.

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Section: Resultsmentioning

confidence: 99%

Aggregation pheromone of the agave weevil, Scyphophorus acupunctatus

Ruiz-Montiel¹,

García‐Coapio²,

Rojas³

et al. 2008

Entomologia Exp Applicata

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“…Análises posteriores em cromatógrafo gasoso acoplado a um espectrômetro de massas (CG-MS) do extrato natural de machos revelaram a presença de três compostos principais: a cetona 23, (E)-6-metil-hept-2-en-4-ona (26) e 2-hidroxi-2-metil-heptan-4-ona (27) (Figura 3), em ordem crescente de abundância. 42 Os compostos 26 e 27 ainda não tinham sido identificados em insetos. Testes de campo mostraram maior atratividade dos insetos para as armadilhas contendo os compostos 23 e 26 e para mistura dos três compostos (23, 26 e 27), mais a planta hospedeira.…”

Section: Metamasius Spinolaeunclassified

Feromônios de agregação em curculionidae (insecta: coleoptera) e sua implicação taxonômica

Ambrogi¹,

Vidal²,

Zarbin³

et al. 2009

Quím. Nova

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Recebido em 26/9/08; aceito em 24/4/09; publicado na web em 7/10/09 AGGREGATION PHEROMONE IN CURCULIONIDAE (INSECTA: COLEOPTERA) AND THEIR TAXONOMIC IMPLICATION. The pheromones of the weevils has been the most studied and used so far for Coleoptera species. The majority of reported weevil pheromones is produced by males and usually attract both sexes. The identified pheromone compounds are classified in two categories: ten-carbon compounds with terpenoid branching and compounds of various sizes, apparently of fatty-acid origin. These pheromone structural categories are consistent within subfamilies. This review aims to give an overview of the aggregation pheromones identified for Curculionidae pests, describing the relationship of the molecules structural pattern among subfamilies, and propose an identification key based on the structure of the pheromone components.Keywords: weevils; pheromone structure; identification key. INTRODUÇÃOOs curculionídeos são besouros da superfamília Curculionoidea, com cerca de 60.000 espécies descritas.1 Os adultos da família Curculionidae são facilmente reconhecidos pela presença de uma cabeça prolongada em um rostro de comprimento muito variável, assim como antenas genículo-clavadas 2 (Figura 1). Os curculionídeos, em sua grande maioria, são fitófagos, tanto na fase larval como na adulta. 3Muitas espécies são importantes pragas de diversas culturas, bem como de grãos armazenados, e a necessidade constante de medidas de controle mais efetivas e seguras ao meio ambiente tem exigido da comunidade científica a busca por novos métodos de controle para minimizar o impacto desses insetos. Como em outros grupos de insetos, esta necessidade tem aumentado a investigação do uso de feromônios como mais uma ferramenta no manejo dessas pragas. Um trabalho extensivo tem sido feito na identificação de feromônios e seu uso dentro da família Curculionidae, 4 exemplificado pelas décadas de pesquisa conduzidas com o bicudo do algodoeiro, Anthonomus grandis.5 Seguindo a identificação de quatro componentes do feromônio de agregação de A. grandis, 6 feromônios de várias espécies de curculionídeos têm sido identificados.A maioria dos feromônios relatada em curculionídeos é produzida pelos machos e usualmente atrai ambos os sexos, por isso são conhecidos como feromônios de agregação. Entretanto, alguns feromônios também têm sido verificados em fêmeas. Estes incluem feromônios sexuais de longa-distância, que atraem principalmente machos, curta-distância ou feromônio de contato, que permitem reconhecer coespecíficos e feromônio de oviposição, que algumas fêmeas usam para marcar os hospedeiros, nos quais elas colocam seus ovos. Finalmente, existem exemplos de curculionídeos utilizando feromônios de outras espécies de besouros, como cairomônios. 4 Em virtude de seus hábitos de vida, o estudo comportamental de curculionídeos é relativamente complexo. Uma alternativa que evita muitos problemas com bioensaios e acelera o processo de identificação é a cromatografia gasosa acoplada à eletroantenografia (CG-EAG)....

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“…Differences in susceptibility of male and female adults of the same species to entomopathogenic fungi have been previously reported (Dimbi et al. 2003), and these differences may be important when developing assisted autodissemination devices such as that being developed against M. spinolae (Roy and Pell 2000; Tafoya et al. 2003, 2007; Vega et al.…”

Section: Discussionmentioning

confidence: 88%

Susceptibility of the cactus weevil Metamasius spinolae to Beauveria bassiana and Metarhizium anisopliae under laboratory and field conditions

Orduño-Cruz

Guzmán‐Franco

Rodríguez‐Leyva

et al. 2011

Journal of Applied Microbiology

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Aims: To evaluate different entomopathogenic fungal isolates against the cactus weevil Metamasius spinolae under laboratory and field conditions, and select an isolate to be used as a tool in the management of this insect pest. Methods and Results: Four experiments were carried out. The effect of temperature on the in vitro growth of eight isolates of entomopathogenic fungi (two Metarhizium anisopliae and six Beauveria bassiana) was assessed. The susceptibility of adult M. spinolae to the same isolates was evaluated. Using three selected isolates, the interaction between susceptibility and sex of the insect was studied. Finally, a field experiment was carried out to evaluate infection of adult M. spinolae by the same three isolates under natural abiotic conditions. Overall, growth rate was greatest at 25°C for all the isolates. In vitro growth of M. anisopliae was greater than B. bassiana. Mortality of adult M. spinolae was greater when inoculated with B. bassiana compared with isolates of M. anisopliae. Susceptibility had no interaction with the sex of the insect. The proportion of insects succumbing to infection was smaller when incubated under field conditions than when incubated under laboratory conditions. Conclusions: The experiments described here showed a complex interaction between entomopathogenic fungi and M. spinolae, and these data allows us to select isolate Bb107 as a first step towards its use in the management of this pest insect. Significance and Impact of the Study: Our results showed that entomopathogenic fungi can be used for the control of M. spinolae, which may help reduce the use of chemical insecticides and, therefore, the exposure of Opuntia ficus‐indica producers to pesticides.

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Aggregation Pheromone of <I>Metamasius spinolae</I> (Coleoptera: Curculionidae): Chemical Analysis and Field Test

Cited by 15 publications

References 11 publications

Aggregation pheromone of the agave weevil, Scyphophorus acupunctatus

Aggregation pheromone of the agave weevil, Scyphophorus acupunctatus

Feromônios de agregação em curculionidae (insecta: coleoptera) e sua implicação taxonômica

Susceptibility of the cactus weevil Metamasius spinolae to Beauveria bassiana and Metarhizium anisopliae under laboratory and field conditions

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