Aggregation and adhesion of gold nanoparticles in phosphate buffered saline

Du, Shangfeng; Kendall, Kevin; Toloueinia, Panteha; Mehrabadi, Yasamin; Gupta, Gaurav; Newton, Jill E.

doi:10.1007/s11051-012-0758-z

Cited by 62 publications

(55 citation statements)

References 27 publications

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“…TEM images of Au nanoparticles in PBS showed substantial agglomeration, (Supplementary Figure S11) justifying the red shift in the plasmon resonance peak compared to those synthesized in presence of K 2 CO 3 . The increased ionic strength of the medium might have led to the agglomeration of the Au nanoparticles synthesized in PBS as previously reported34. The sizes of all the metallic nanoparticles (Au, Ag and Pt) synthesized using urease are well in accordance with the sizes of the nanoparticles synthesized using biosynthetic routes involving natural reducing agents.…”

Section: Resultssupporting

confidence: 81%

Biogenic Growth of Alloys and Core-Shell Nanostructures Using Urease as a Nanoreactor at Ambient Conditions

Sharma

Mandani

Sarma

2013

Sci Rep

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Biomineralization is an extremely efficient biologically guided process towards the advancement of nano-bio integrated materials. As a prime module of the natural world, enzymes are expected to play a major role in biogenic growth of inorganic nanostructures. Although there have been developments in designing enzyme-responsive nanoparticle systems or generation of inorganic nanostructures in an enzyme-stimulated environment, reports regarding action of enzymes as reducing agents themselves for the growth of inorganic nanoparticles still remains elusive. Here we present a mechanistic investigation towards the synthesis of metal and metallic alloy nanoparticles using a commonly investigated enzyme, Jack bean urease (JBU), as a reducing as well as stabilizing agent under physiological conditions. The catalytic functionality of urease was taken advantage of towards the development of metal-ZnO core-shell nanocomposites, making urease an ideal bionanoreactor for synthesizing higher order nanostructures such as alloys and core- shell under ambient conditions.

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Section: Resultssupporting

confidence: 81%

Biogenic Growth of Alloys and Core-Shell Nanostructures Using Urease as a Nanoreactor at Ambient Conditions

Sharma

Mandani

Sarma

2013

Sci Rep

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“…This ultimately causes destabilization, which is mediated through random van der Waals interactions. 40,41 Methylcellulose is an amphiphilic molecule, which is commonly employed in stabilizing hydrophobic carbon nanomaterials, especially uncharged carbon nanotubes. 42 However, in the case of cationic NPs, it can reduce the positive charge density of the surface by masking the functional groups through adsorption.…”

Section: Dispersion Stability Of Lysine Nds In Formulationsmentioning

confidence: 99%

Lysine-functionalized nanodiamonds as gene carriers: development of stable colloidal dispersion for in vitro cellular uptake studies and siRNA delivery application

Alwani¹,

Kaur²,

Michel³

et al. 2016

IJN

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Purpose Nanodiamonds (NDs) are emerging as an attractive tool for gene therapeutics. To reach their full potential for biological application, NDs should maintain their colloidal stability in biological milieu. This study describes the behavior of lysine-functionalized ND (lys-ND) in various dispersion media, with an aim to limit aggregation and improve the colloidal stability of ND-gene complexes called diamoplexes. Furthermore, cellular and macromolecular interactions of lys-NDs are also analyzed in vitro to establish the understanding of ND-mediated gene transfer in cells. Methods lys-NDs were synthesized earlier through covalent conjugation of lysine amino acid to carboxylated NDs surface generated through re-oxidation in strong oxidizing acids. In this study, dispersions of lys-NDs were prepared in various media, and the degree of sedimentation was monitored for 72 hours. Particle size distributions and zeta potential measurements were performed for a period of 25 days to characterize the physicochemical stability of lys-NDs in the medium. The interaction profile of lys-NDs with fetal bovine serum showed formation of a protein corona, which was evaluated by size and charge distribution measurements. Uptake of lys-NDs in cervical cancer cells was analyzed by scanning transmission X-ray microscopy, flow cytometry, and confocal microscopy. Cellular uptake of diamoplexes (complex of lys-NDs with small interfering RNA) was also analyzed using flow cytometry. Results Aqueous dispersion of lys-NDs showed minimum sedimentation and remained stable over a period of 25 days. Size distributions showed good stability, remaining under 100 nm throughout the testing period. A positive zeta potential of >+20 mV indicated a preservation of surface charges. Size distribution and zeta potential changed for lys-NDs after incubation with blood serum, suggesting an interaction with biomolecules, mainly proteins, and a possible formation of a protein corona. Cellular internalization of lys-NDs was confirmed by various techniques such as confocal microscopy, soft X-ray spectroscopy, and flow cytometry. Conclusion This study establishes that dispersion of lys-NDs in aqueous medium maintains long-term stability and also provides evidence that lysine functionalization enables NDs to interact effectively with the biological system to be used for RNAi therapeutics.

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“…Citrate stabilizes AuNPs electrostatically, via charge repulsion. In cell culture medium or other simulated extracellular fluids of high ionic strength, ions in solution shield the charges, reducing colloidal stability (Du et al, 2012). This was observed immediately when 60 nm AuNPs were dispersed.…”

Section: Discussionmentioning

confidence: 99%

Citrate-stabilized gold nanoparticles as negative controls for measurements of neurite outgrowth

Jeerage

Oreskovic

Curtin

et al. 2015

Toxicology in Vitro

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Aggregation and adhesion of gold nanoparticles in phosphate buffered saline

Cited by 62 publications

References 27 publications

Biogenic Growth of Alloys and Core-Shell Nanostructures Using Urease as a Nanoreactor at Ambient Conditions

Biogenic Growth of Alloys and Core-Shell Nanostructures Using Urease as a Nanoreactor at Ambient Conditions

Lysine-functionalized nanodiamonds as gene carriers: development of stable colloidal dispersion for in vitro cellular uptake studies and siRNA delivery application

Citrate-stabilized gold nanoparticles as negative controls for measurements of neurite outgrowth

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