2014
DOI: 10.18632/aging.100708 View full text |Buy / Rent full text
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Abstract: Reprogramming of human fibroblasts into induced pluripotent stem cells (iPSCs) leads to mitochondrial rejuvenation, making iPSCs a candidate model to study the mitochondrial biology during stemness and differentiation. At present, it is generally accepted that iPSCs can be maintained and propagated indefinitely in culture, but no specific studies have addressed this issue. In our study, we investigated features related to the 'biological age' of iPSCs, culturing and analyzing iPSCs kept for prolonged periods i… Show more

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“…These data emphasize that different levels of mitochondrial maturation and metabolism predilection exist, depending on the pluripotency stage. In the same vein, iPSCs maintained for extended in vitro culture periods display increased mitochondrial content and reduced potential to undergo neurogenesis in comparison with young iPSCs [25]. These data emphasize the importance of controlling the precise pluripotency stage and time in culture of stem cells in studies interested in their pluripotency and metabolic features.…”
Section: Opposite Mitochondrial Remodeling and Metabolic Shifts Durinmentioning
“…As adult neural stem cells differentiate in vitro to neurons they also decrease aerobic glycolysis though a HIF1α-independent mechanism [25]. Interestingly pre-neural cells that retain an elevated number of mitochondria have a neuronal stem cell differentiation defect [29], emphasizing the developmental link between metabolic flux and cell fate decisions.…”
Section: Introductionmentioning
“…[27][28][29][30][31] Hence, comprehensive testing of iPSCs and their potential aging signature should be conducted. In the current study, we sought to thoroughly investigate the aging characteristics of iPSCs derived from aged somatic cells.…”
Section: Discussionmentioning
“…Therefore, to shed light on these issues, we investigated some aspects of mitochondrial aging and their effects on neuronal differentiation [5]. In particular, we compared the mitochondrial network of ‘young’ and ‘aged’ iPSCs (y- and a-iPSCs, cultured respectively one month and one year) both in self-renewal and neural differentiating conditions.…”
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“…Mitochondria were depolarized and formed a convoluted network that occupied most of the cytoplasmic space ultimately leading cells to apoptosis. Furthermore, a-iPSCs showed an abnormal expression pattern of genes involved in mitochondrial biogenesis and functionality [5]. …”
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