2018
DOI: 10.18632/aging.101472
| View full text |Cite|
|
Sign up to set email alerts
|

Abstract: It remains unclear how exercise, as an entropic event, brings benefit against human aging. Here we examined longitudinal changes of p16Ink4a+ senescent cells in skeletal muscle of young men (aged 22.5±1.7 y) before and after resistance exercise (0 h and 48 h) with multiple biopsies at two different protein availabilities: low protein (14%) and isocaloric high protein (44%) supplemented conditions. Immunohistochemistry analysis of muscle cross-sections using p16Ink4a and CD34 antibodies confirmed that the detec… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
24
1

Year Published

2019
2019
2023
2023

Publication Types

Select...
4
2

Relationship

1
5

Authors

Journals

citations
Cited by 27 publications
(25 citation statements)
references
References 22 publications
0
24
1
Order By: Relevance
“…Although many p16 antibodies used in previous studies of senescence are no longer available, 5,6,35 we utilized three different commercially available p16 antibodies. One, used by various labs for immunohistochemical analysis of p16, 36‐39 labeled > 60% of all nuclei in both young and old muscle (Figure S1), in spite of the fact that p16 mRNA expression is low in muscle, as is p21 mRNA (CT values 35‐36; data not shown). Consistent with mRNA results, two of the antibodies detected no p16 + cells in human skeletal muscle, regardless of age (data not shown).…”
Section: Resultsmentioning
confidence: 98%
“…Although many p16 antibodies used in previous studies of senescence are no longer available, 5,6,35 we utilized three different commercially available p16 antibodies. One, used by various labs for immunohistochemical analysis of p16, 36‐39 labeled > 60% of all nuclei in both young and old muscle (Figure S1), in spite of the fact that p16 mRNA expression is low in muscle, as is p21 mRNA (CT values 35‐36; data not shown). Consistent with mRNA results, two of the antibodies detected no p16 + cells in human skeletal muscle, regardless of age (data not shown).…”
Section: Resultsmentioning
confidence: 98%
“…Despite the evolvement as a highly specialized multicellular system by populating cells can increase fitness of the human body, persistent growth together with senescent cell The illustration is reproduced according to Yang et al [6] accumulation can be the fundamental cause of aging and eventual death. Exercise accelerates death of unfit senescent cells in the human body and slows down weight gain during early stage of a multicellular life.…”
Section: Resultsmentioning
confidence: 99%
“…The protein p16 INK4a is a widely used senescence marker for replicable cells, which increases as tissue ages [14]. We recently reported decreased senescent cells in capillaries of human skeletal muscle immediately after and 48 h after resistance exercise [6] (Fig. 3), suggesting death of senescent cells in tissue after exercise ( Fig.…”
Section: Resistance Exercise Eliminates Senescent Cells In Human Bodymentioning
confidence: 94%
See 2 more Smart Citations