Age-related micro-RNA abundance in individual C. elegans

Lucanic, Mark; Graham, Jill; Scott, Gary K.; Bhaumik, Dipa; Benz, Christopher C.; Hubbard, Alan; Lithgow, Gordon J.; Melov, Simon

doi:10.18632/aging.100564

Cited by 23 publications

(12 citation statements)

References 35 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nine of the 13 germline miRNA candidates identified here are among the 50+ miRNAs that change in abundance as hermaphrodites age and cease reproduction (Kato et al, 2011; Lucanic et al, 2013). In particular, six members of the miR35 family dramatically increase in abundance in aged, post-reproductive worms, while miR-70, −244 , and −788 decrease.…”

Section: Discussionmentioning

confidence: 99%

“…Despite elegant studies of miRNAs in C. elegans identifying miRNAs that are differentially expressed during development (Kato et al, 2009) or with ageing (Kato et al, 2011; Lucanic et al, 2013), no studies targeting germline miRNAs have been reported. These studies were likely hindered by the inherent germline silencing of C. elegans transgenes, resulting in an inability of miRNA transgenes to express in the germline (Kelly et al, 1999; Martinez et al, 2008; Pierce et al, 2008; Shaw et al, 2010) and because in situ hybridizations for miRNAs in C. elegans had not yet been published, with the exception of miR-57 , the most abundant miRNA in our RNA-seq studies, Supplemental Table S1, localizing to the posterior of a late-stage embryo (Zhao et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Small RNA in situ hybridization in Caenorhabditis elegans, combined with RNA-seq, identifies germline-enriched microRNAs

McEwen

Yao

Yun³

et al. 2016

Developmental Biology

View full text Add to dashboard Buy / Rent full text

Over four hundred different microRNAs (miRNAs) have been identified in the genome of the model organism the nematode Caenorhabditis elegans. As the germline is dedicated to the preservation of each species, and almost half of all the cells in an adult nematode are germline, it is likely that regulatory miRNAs are important for germline development and maintenance. In C. elegans the miR35 family has strong maternal effects, contributing to normal embryogenesis and to adult fecundity. To determine whether any particular miRNAs are greatly enriched in the C. elegans germline we used RNA-seq to compare the miRNA populations in several germline-defective strains of adult C. elegans worms, including glp-4(germline proliferation-4), glh-1(germline helicase-1) and dcr-1(dicer-1). Statistical analyses of RNA-seq comparisons identified 13 miRNAs that are germline-enriched, including seven members of the well-studied miR35 family that were reduced as much as 1000-fold in TaqMan qRT PCR miRNA assays. Along with the miR35s, six others: miR-56 (a member of the miR51 family),−70, −244, −260 , −788 and −4813, none of which previously considered as such, were also identified by RNA-seq as germline-enriched candidates. We went on to develop a successful miRNA in situ hybridization protocol for C. elegans, revealing miR35s specifically concentrate during oogenesis in the pachytene region of the gonad, and persist throughout early embryogenesis, while in adult animals neither let-7 nor miR-228 has a germline-bias.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Small RNA in situ hybridization in Caenorhabditis elegans, combined with RNA-seq, identifies germline-enriched microRNAs

McEwen

Yao

Yun³

et al. 2016

Developmental Biology

View full text Add to dashboard Buy / Rent full text

show abstract

“…The expression of many noncoding RNAs, including microRNAs (miRNAs), Piwi‐interacting RNAs (piRNAs), transfer RNAs (tRNAs), ribosomal RNAs (rRNAs), small nucleolar RNAs (snoRNAs), and circular RNAs (circRNAs), changes with age (Cortes‐Lopez et al, ; de Lencastre et al, ; Ibanez‐Ventoso et al, ; Inukai & Slack, ; Kato & Slack, ; Kato et al, ; Lucanic et al, ). The overall levels of miRNAs tend to decrease during aging (de Lencastre et al, ; Ibanez‐Ventoso et al, ), and this appears to be caused by age‐dependent reductions in the mRNA levels of dcr‐1 /Dicer (Mori et al, ) and alg‐1 /Argonaut (Inukai, Pincus, de Lencastre, & Slack, ).…”

Section: Age‐dependent Changesmentioning

confidence: 99%

Age‐dependent changes and biomarkers of aging inCaenorhabditis elegans

et al. 2019

View full text Add to dashboard Buy / Rent full text

Caenorhabditis elegans is an exceptionally valuable model for aging research because of many advantages, including its genetic tractability, short lifespan, and clear age‐dependent physiological changes. Aged C. elegans display a decline in their anatomical and functional features, including tissue integrity, motility, learning and memory, and immunity. Caenorhabditis elegans also exhibit many age‐associated changes in the expression of microRNAs and stress‐responsive genes and in RNA and protein quality control systems. Many of these age‐associated changes provide information on the health of the animals and serve as valuable biomarkers for aging research. Here, we review the age‐dependent changes in C. elegans and their utility as aging biomarkers indicative of the physiological status of aging.

show abstract

“…Regular lifespan assays (non-multiwell, high-throughput, or automated lifespan machine based) were performed as previously described (Lucanic et al, 2013b). In brief, synchronized (from 2-h egg lays) worm populations were added to 3 cM NGM plates supplemented with 10 lg/ mL 5-fluoro-2 0 -deoxyuridine (FUdR) at day one of adulthood.…”

Section: Lifespan Assaysmentioning

confidence: 99%

Chemical activation of a food deprivation signal extends lifespan

Lucanic

Garrett

et al. 2016

Aging Cell

Self Cite

View full text Add to dashboard Buy / Rent full text

SummaryModel organisms subject to dietary restriction (DR) generally live longer. Accompanying this lifespan extension are improvements in overall health, based on multiple metrics. This indicates that pharmacological treatments that mimic the effects of DR could improve health in humans. To find new chemical structures that extend lifespan, we screened 30 000 synthetic, diverse drug‐like chemicals in Caenorhabditis elegans and identified several structurally related compounds that acted through DR mechanisms. The most potent of these NP1 impinges upon a food perception pathway by promoting glutamate signaling in the pharynx. This results in the overriding of a GPCR pathway involved in the perception of food and which normally acts to decrease glutamate signals. Our results describe the activation of a dietary restriction response through the pharmacological masking of a novel sensory pathway that signals the presence of food. This suggests that primary sensory pathways may represent novel targets for human pharmacology.

show abstract

Age-related micro-RNA abundance in individual C. elegans

Cited by 23 publications

References 35 publications

Small RNA in situ hybridization in Caenorhabditis elegans, combined with RNA-seq, identifies germline-enriched microRNAs

Small RNA in situ hybridization in Caenorhabditis elegans, combined with RNA-seq, identifies germline-enriched microRNAs

Age‐dependent changes and biomarkers of aging inCaenorhabditis elegans

Chemical activation of a food deprivation signal extends lifespan

Contact Info

Product

Resources

About