2013
DOI: 10.18632/aging.100564
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Abstract: Non-coding small RNAs of the micro-RNA class (miRNA) are conserved regulators of gene function with a broad impact on biological processes. We screened miRNA levels for age-related changes in individual worms and investigated their influence on the lifespan of the nematode C. elegans. We measured the abundance of 69 miRNAs expressed in individual animals at different ages with over thirty five thousand discrete quantitative nano-fluidic polymerase chain reactions. We found that miRNA abundance was highly varia… Show more

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Cited by 23 publications
(12 citation statements)
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References 35 publications
(49 reference statements)
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“…Nine of the 13 germline miRNA candidates identified here are among the 50+ miRNAs that change in abundance as hermaphrodites age and cease reproduction (Kato et al, 2011; Lucanic et al, 2013). In particular, six members of the miR35 family dramatically increase in abundance in aged, post-reproductive worms, while miR-70, −244 , and −788 decrease.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Nine of the 13 germline miRNA candidates identified here are among the 50+ miRNAs that change in abundance as hermaphrodites age and cease reproduction (Kato et al, 2011; Lucanic et al, 2013). In particular, six members of the miR35 family dramatically increase in abundance in aged, post-reproductive worms, while miR-70, −244 , and −788 decrease.…”
Section: Discussionmentioning
confidence: 99%
“…Despite elegant studies of miRNAs in C. elegans identifying miRNAs that are differentially expressed during development (Kato et al, 2009) or with ageing (Kato et al, 2011; Lucanic et al, 2013), no studies targeting germline miRNAs have been reported. These studies were likely hindered by the inherent germline silencing of C. elegans transgenes, resulting in an inability of miRNA transgenes to express in the germline (Kelly et al, 1999; Martinez et al, 2008; Pierce et al, 2008; Shaw et al, 2010) and because in situ hybridizations for miRNAs in C. elegans had not yet been published, with the exception of miR-57 , the most abundant miRNA in our RNA-seq studies, Supplemental Table S1, localizing to the posterior of a late-stage embryo (Zhao et al, 2010).…”
Section: Introductionmentioning
confidence: 99%
“…The expression of many noncoding RNAs, including microRNAs (miRNAs), Piwi‐interacting RNAs (piRNAs), transfer RNAs (tRNAs), ribosomal RNAs (rRNAs), small nucleolar RNAs (snoRNAs), and circular RNAs (circRNAs), changes with age (Cortes‐Lopez et al, ; de Lencastre et al, ; Ibanez‐Ventoso et al, ; Inukai & Slack, ; Kato & Slack, ; Kato et al, ; Lucanic et al, ). The overall levels of miRNAs tend to decrease during aging (de Lencastre et al, ; Ibanez‐Ventoso et al, ), and this appears to be caused by age‐dependent reductions in the mRNA levels of dcr‐1 /Dicer (Mori et al, ) and alg‐1 /Argonaut (Inukai, Pincus, de Lencastre, & Slack, ).…”
Section: Age‐dependent Changesmentioning
confidence: 99%
“…Regular lifespan assays (non-multiwell, high-throughput, or automated lifespan machine based) were performed as previously described (Lucanic et al, 2013b). In brief, synchronized (from 2-h egg lays) worm populations were added to 3 cM NGM plates supplemented with 10 lg/ mL 5-fluoro-2 0 -deoxyuridine (FUdR) at day one of adulthood.…”
Section: Lifespan Assaysmentioning
confidence: 99%