2017
DOI: 10.18632/aging.101333
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Abstract: Gene expression is influenced by both genetic variants and the environment. As individuals age, changes in gene expression may be associated with decline in physical and cognitive abilities. We measured transcriptome-wide expression levels in lymphoblastoid cell lines derived from members of the Lothian Birth Cohort 1936 at mean ages 70 and 76 years. Changes in gene expression levels were identified for 1,741 transcripts in 434 individuals. Gene Ontology enrichment analysis indicated an enrichment of biologica… Show more

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Cited by 29 publications
(25 citation statements)
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References 64 publications
(68 reference statements)
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“…A similar result was obtained in other analysis using several aging organs -kidney, lung, brain cortex, liver-from humans, mice, and rats [7]. In human lymphoblastoid cells, the proportion was 1:1 [31]. Two studies using whole blood cells found a proportion close to 1.4:1 [32,33].…”
Section: General Transcriptomic Landscape Of Aging In Pfcsupporting
confidence: 86%
“…A similar result was obtained in other analysis using several aging organs -kidney, lung, brain cortex, liver-from humans, mice, and rats [7]. In human lymphoblastoid cells, the proportion was 1:1 [31]. Two studies using whole blood cells found a proportion close to 1.4:1 [32,33].…”
Section: General Transcriptomic Landscape Of Aging In Pfcsupporting
confidence: 86%
“…Gene expression data. Expression data were acquired for the 876 LBC1936 individuals 48 . In summary, peripheral blood mononuclear cells (PBMCs) from 1091 LBC1936 individuals were extracted from whole blood collected at mean age 70 years (S.D.…”
Section: Methodsmentioning
confidence: 99%
“…Frozen LCL pellets were returned to the ECRF Genetics Core, where RNA was extracted using a Qiagen miRNeasy kit, biotin-labelled, and genome-wide gene expression levels measured using Illumina's HumanHT-12 v4 Expression BeadChip which contains 47,231 probes; a control RNA sample was included on each array. Individuals with a signal-to-noise ratio <10 or with fewer than 9000 detected transcripts (P < 0.01) were excluded (n = 130), and only probes expressed in >20% of individuals were retained 48 . The microarray data was quantile normalised using the Bioconductor pacakage lumi 49 , after which probes with no detectable expression were removed, resulting in 38,764 probes.…”
Section: Methodsmentioning
confidence: 99%
“…Aging of multi-cellular organisms is a complex biological process involving various molecular alterations such as epigenetic [1], genetic [2], and transcriptional changes [3][4][5][6]. These changes are likely modulated by various environmental factors that can either induce premature aging [7] or decelerate the natural aging rate [8].…”
Section: Introductionmentioning
confidence: 99%