Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons

Carrascal, Livia; Gorton, Ella; Pardillo-Díaz, Ricardo; Pérez-García, Patricia; Gómez-Oliva, Ricardo; Castro, Carmen; Nuñez-Abades, Pedro

doi:10.3390/antiox9121307

Cited by 4 publications

(4 citation statements)

References 64 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to previous studies, oxidative stress vulnerability increases with age, with adults being more vulnerable and juveniles being partially resistant (30,31). In the present study, effects of 1 mM H 2 O 2 on GnRH neurons were studied in three groups according to age: juvenile, 8 to 25 postnatal days (PND); peripubertal, 26 to 45 PND; and adults, more than 60 PND.…”

Section: Hydrogen Peroxide Exposure Induces Variegated Response In Gn...mentioning

confidence: 84%

“…However, unlike adults, most immature GnRH neurons were stimulated by the same concentration of H 2 O 2 . According to previous studies, oxidative stress vulnerability increase with age, with young rats being more resistant to ROS than adults (30). Furthermore, H 2 O 2 has both excitatory and inhibitory effects on neuronal excitability depending on neuronal population and brain location (43).…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Hydrogen peroxide suppresses excitability of gonadotropin-releasing hormone neurons in adult mouse

Rijal

Jang²,

Cho³

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

It has been reported that reactive oxygen species (ROS) derived from oxygen molecule reduction can interfere with the cross-talk between the hypothalamic-pituitary-gonadal (HPG) axis and other endocrine axes, thus affecting fertility. Furthermore, ROS have been linked to GnRH receptor signaling in gonadotropes involved in gonadotropin release. There has been evidence that ROS can interfere with the HPG axis and gonadotropin release at various levels. However, the direct effect of ROS on gonadotropin-releasing hormone (GnRH) neuron remains unclear. Thus, the objective of this study was to determine the effect of hydrogen peroxide (H2O2), an ROS source, on GnRH neuronal excitabilities in transgenic GnRH-green fluorescent protein-tagged mice using the whole-cell patch-clamp electrophysiology. In adults, H2O2 at high concentrations (mM level) hyperpolarized most GnRH neurons tested, whereas low concentrations (pM to μM) caused slight depolarization. In immature GnRH neurons, H2O2 exposure induced excitation. The sensitivity of GnRH neurons to H2O2 was increased with postnatal development. The effect of H2O2 on adult female GnRH neurons was found to be estrous cycle-dependent. Hyperpolarization mediated by H2O2 persisted in the presence of tetrodotoxin, a voltage-gated Na+ channel blocker, and amino-acids receptor blocking cocktail containing blockers for the ionotropic glutamate receptors, glycine receptors, and GABAA receptors, indicating that H2O2 could act on GnRH neurons directly. Furthermore, glibenclamide, an ATP-sensitive K+ (KATP) channel blocker, completely blocked H2O2-mediated hyperpolarization. Increasing endogenous H2O2 by inhibiting glutathione peroxidase decreased spontaneous activities of most GnRH neurons. We conclude that ROS can act as signaling molecules for regulating GnRH neuron’s excitability and that adult GnRH neurons are sensitive to increased ROS concentration. Results of this study demonstrate that ROS have direct modulatory effects on the HPG axis at the hypothalamic level to regulate GnRH neuron’s excitabilities.

show abstract

Section: Hydrogen Peroxide Exposure Induces Variegated Response In Gn...mentioning

confidence: 84%

Section: Discussionmentioning

confidence: 97%

Hydrogen peroxide suppresses excitability of gonadotropin-releasing hormone neurons in adult mouse

Rijal

Jang²,

Cho³

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

“…Using a micromanipulator (MP-225, Sutter Instrument, CA, United States), micropipettes were visually placed in the motor cortex area. Within this region the pyramidal neurons were identified based on their characteristic morphology ( Pardillo-Díaz et al, 2016 ; Carrascal et al, 2020 ). An amplified (MultiClamp 700B, Axon Instruments, Molecular Devices, Sunnyvale, CA, United States) was also used to achieve the whole-cell patch clamp configuration.…”

Section: Methodsmentioning

confidence: 99%

Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development

Pérez-García

Pardillo-Díaz

Geribaldi-Doldán

et al. 2021

Front. Mol. Neurosci.

Self Cite

View full text Add to dashboard Cite

Achieving the distinctive complex behaviors of adult mammals requires the development of a great variety of specialized neural circuits. Although the development of these circuits begins during the embryonic stage, they remain immature at birth, requiring a postnatal maturation process to achieve these complex tasks. Understanding how the neuronal membrane properties and circuits change during development is the first step to understand their transition into efficient ones. Thus, using whole cell patch clamp recordings, we have studied the changes in the electrophysiological properties of layer V pyramidal neurons of the rat primary motor cortex during postnatal development. Among all the parameters studied, only the voltage threshold was established at birth and, although some of the changes occurred mainly during the second postnatal week, other properties such as membrane potential, capacitance, duration of the post-hyperpolarization phase or the maximum firing rate were not defined until the beginning of adulthood. Those modifications lead to a decrease in neuronal excitability and to an increase in the working range in young adult neurons, allowing more sensitive and accurate responses. This maturation process, that involves an increase in neuronal size and changes in ionic conductances, seems to be influenced by the neuronal type and by the task that neurons perform as inferred from the comparison with other pyramidal and motor neuron populations.

show abstract

“…Therefore, based on all our previous results, it is reasonable to hypothesize that damage caused by oxidants such as CH mainly contributes to neuronal dysfunction in aged brains, and therefore, neurodegenerative diseases such as ALS are more likely to occur in the older population [ 140 ].…”

Section: Impact Of Oxidative Stress In Motor Cortex Neurons Hyperexci...mentioning

confidence: 99%

Oxidative Stress as a Potential Mechanism Underlying Membrane Hyperexcitability in Neurodegenerative Diseases

et al. 2022

Self Cite

View full text Add to dashboard Cite

Neurodegenerative diseases are characterized by gradually progressive, selective loss of anatomically or physiologically related neuronal systems that produce brain damage from which there is no recovery. Despite the differences in clinical manifestations and neuronal vulnerability, the pathological processes appear to be similar, suggesting common neurodegenerative pathways. It is well known that oxidative stress and the production of reactive oxygen radicals plays a key role in neuronal cell damage. It has been proposed that this stress, among other mechanisms, could contribute to neuronal degeneration and might be one of the factors triggering the development of these pathologies. Another common feature in most neurodegenerative diseases is neuron hyperexcitability, an aberrant electrical activity. This review, focusing mainly on primary motor cortex pyramidal neurons, critically evaluates the idea that oxidative stress and inflammation may be involved in neurodegeneration via their capacity to increase membrane excitability.

show abstract

Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons

Cited by 4 publications

References 64 publications

Hydrogen peroxide suppresses excitability of gonadotropin-releasing hormone neurons in adult mouse

Hydrogen peroxide suppresses excitability of gonadotropin-releasing hormone neurons in adult mouse

Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development

Oxidative Stress as a Potential Mechanism Underlying Membrane Hyperexcitability in Neurodegenerative Diseases

Contact Info

Product

Resources

About