Age alters gait compensations following meniscal injury in male rats

Chan, Kiara M.; Yeater, Taylor; Allen, Kyle D.

doi:10.1002/jor.25306

Cited by 7 publications

(8 citation statements)

References 38 publications

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“…The copyright holder for this preprint (which this version posted April 19, 2023. ; https://doi.org/10.1101/2023.04.19.537488 doi: bioRxiv preprint uncertain. In previous gait studies conducted by our group, velocity increased across testing time points regardless of surgical grouping, potentially due to repeated exposure to gait testing (Chan et al, 2022;Kloefkorn et al, 2015). Analysis of repeated test exposures has also been performed in other classical behavioral assays such as the elevated plus maze, elevated zero maze, forced swim, open-field, and light/dark exploration test, but with largely mixed results depending on the type of tests, type of animal used, and frequency of exposures (Blokland et al, 2012;Bodden et al, 2018;Tucker and McCabe, 2017).…”

Section: Discussionmentioning

confidence: 68%

“…In total, each animal was tested at least 6 times. The (Chan et al, 2022). Age and weight were recorded at the time of testing.…”

Section: Animalsmentioning

confidence: 99%

“…Rodent gait analysis is a powerful preclinical assay to characterize behavioral changes associated with movement-related pain and disability. Gait analysis for rodent models has been particularly useful for identifying behavioral changes that manifest due to Parkinson's disease (Amende et al, 2005;Boix et al, 2018), spinal cord injury (Isvoranu et al, 2021;Pukale et al, 2021), osteoarthritis (Chan et al, 2022;Jacobs et al, 2014;Lakes and Allen, 2018), and other neurodevelopmental disorders (Akula et al, 2020). Detecting gait changes in rodents is not trivial, as gait analysis must be highly sensitive due to the small size and rapidity of the rodent gait sequence in comparison to humans.…”

Section: Introductionmentioning

confidence: 99%

“…Here, body weight is often used as a correlate to an animal's skeletal size, where weight can affect spatial gait parameters such as stride length and step width (Pitzer et al, 2021;Sheppard et al, 2022). Similarly, age affects both velocity over time and disease-related shifts in gait parameters (Bair et al, 2019;Broom et al, 2021;Chan et al, 2022). Careful consideration of these factors through experimental design and data analysis is key to interpreting gait data.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Repeat Test Exposure on Gait Parameters in Naïve Lewis Rats

Allen

Thurlow

Chan

et al. 2023

Preprint

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Rodent gait analysis has emerged as a powerful, quantitative behavioral assay to characterize the pain and disability associated with movement-related disorders. In other behavioral assays, the importance of acclimation and the effect of repeated testing have been evaluated. However, for rodent gait analysis, the effects of repeated gait testing and other environmental factors have not been thoroughly characterized. In this study, fifty-two naive male Lewis rats ages 8 to 42 weeks completed gait testing at semi-random intervals for 31 weeks. Gait videos and force plate data were collected and processed using a custom MATLAB suite to calculate velocity, stride length, step width, percentage stance time (duty factor), and peak vertical force data. Exposure was quantified as the number of gait testing sessions. Linear mixed effects models were used to evaluate the effects of velocity, exposure, age, and weight on animal gait patterns. Relative to age and weight, repeated exposure was the dominant parameter affecting gait variables with significant effects on walking velocity, stride length, fore and hind limb step width, fore limb duty factor, and peak vertical force. From exposure 1 to 7, average velocity increased by approximately 15 cm/s. Together, these data indicate arena exposure had large effects on gait parameters and should be considered in acclimation protocols, experimental design, and subsequent data analysis of rodent gait data.

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Section: Discussionmentioning

confidence: 68%

“…In total, each animal was tested at least 6 times. The (Chan et al, 2022). Age and weight were recorded at the time of testing.…”

Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Repeat Test Exposure on Gait Parameters in Naïve Lewis Rats

Allen

Thurlow

Chan

et al. 2023

Preprint

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“…This velocity increase over time is typical in rodents and has been previously observed in other rodent gait studies. 26 , 27 Here, velocities increased from 39.64 ± 2.12 cm/s at baseline to 47.88±2.22 cm/s by week 12 (error presented as ±95% confidence interval).…”

Section: Resultsmentioning

confidence: 95%

African Spiny Mice (Acomys) Exhibit Mild Osteoarthritis Following Meniscal Injury

et al. 2023

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Objective Hyaline cartilage has limited innate healing abilities and hyaline cartilage loss is a hallmark of osteoarthritis (OA). Animal models can provide important insights into cartilage regeneration potential. One such animal model, the African spiny mouse ( Acomys), is capable of regenerating skin, skeletal muscle, and elastic cartilage. This study aims to evaluate whether these regenerative abilities protect Acomys with meniscal injury from OA-related joint damage and behaviors indicative of joint pain and dysfunction. Design Acomys received destabilization of the medial meniscus (DMM) surgery ( n = 11) or a skin incision ( n = 10). Gait testing occurred at 4, 6, 8, 10, and 12 weeks after surgery. At endpoint, joints were processed for histology to assess cartilage damage. Results Following joint injury, Acomys with DMM surgery altered their walking patterns by increasing the percent stance time on the contralateral limb relative to the operated limb, thereby reducing the amount of time the injured limb must bear weight on its own throughout the gait cycle. Histological grading indicated evidence of OA-related joint damage in Acomys with DMM surgery; these changes were primarily driven by loss of structural integrity in the hyaline cartilage. Conclusions Acomys developed gait compensations, and the hyaline cartilage in Acomys is not fully protected from OA-related joint damage following meniscal injury, although this damage was less severe than that historically found in C57BL/6 mice with an identical injury. Thus, Acomys do not appear to be completely protected from OA-related changes, despite the ability to regenerate other wounded tissues.

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