Agar-supported lipid bilayers — basic structures for biosensor design. Electrical and mechanical properties

Ziegler, W.; Gaburjáková, Jana; Gaburjáková, Marta; Sivak, Branislav; Řeháček, V.; Tvarožek, V.; Hianik, Tibor

doi:10.1016/s0927-7757(97)00292-6

Cited by 28 publications

(25 citation statements)

References 17 publications

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“…8 Recent progress has been made by stabilizing such membrane architectures either by crystallization of S-layer proteins or deposition of hydrogel cushions or other polymer layers. [9][10][11] The membrane lifetime could be enhanced to days; [12][13][14] however the resulting lifetime improvement remains insufficient to allow for continuous monitoring over periods of months, which may be a requirement for some applications.…”

Section: Introductionmentioning

confidence: 99%

Stable insulating tethered bilayer lipid membranes

et al. 2008

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Tethered bilayer lipid membranes have been shown to be an excellent model system for biological membranes. Coupling of a membrane to a solid supports creates a stable system that is accessible for various surface analytical tools. Good electrical sealing properties also enable the use of the membranes in practical sensing applications. The authors have shown that tethered membranes have extended lifetimes up to several months. Air-stability of the bilayer can be achieved by coating the membrane with a hydrogel. The structure of a monolayer and its stability under applied dc potentials have been investigated by neutron scattering. © 2008 American Vacuum Society.

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Section: Introductionmentioning

confidence: 99%

Stable insulating tethered bilayer lipid membranes

et al. 2008

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“…[15][16][17] These BLMs have attracted increasing interest due to their mechanical stability, which affords fabricating robust chemical sensors and molecular electronic devices.…”

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confidence: 99%

“…17 The recordings of ion translocation so far reported as changes in the transmembrane ion currents [12][13][14][16][17][18] and the membrane potential, 15 have utilized mostly hydrogel-supported BLMs in which bioelements were embedded. However, few studies 13,14 have reported single-channel recordings, where a high electric resistance is essential for discriminating a singlechannel current at the level of a few pA from the background one.…”

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confidence: 99%

“…[15][16][17] These BLMs have attracted increasing interest due to their mechanical stability, which affords fabricating robust chemical sensors and molecular electronic devices. 7 However, the metal-supported BLMs lack an aqueous phase on the metal support side, and hence ion translocation across the BLMs is not feasible, and the microscopically rough metal surface causes a local disturbance of the membrane structure, and consequently leaks.…”

mentioning

confidence: 99%

“…7 However, the metal-supported BLMs lack an aqueous phase on the metal support side, and hence ion translocation across the BLMs is not feasible, and the microscopically rough metal surface causes a local disturbance of the membrane structure, and consequently leaks. 17 The recordings of ion translocation so far reported as changes in the transmembrane ion currents [12][13][14][16][17][18] and the membrane potential, 15 have utilized mostly hydrogel-supported BLMs in which bioelements were embedded. However, few studies 13,14 have reported single-channel recordings, where a high electric resistance is essential for discriminating a singlechannel current at the level of a few pA from the background one.…”

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confidence: 99%

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Single-Channel Recordings of Gramicidin at Agarose-Supported Bilayer Lipid Membranes Formed by the Tip-Dip and Painting Methods

et al. 2004

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Recordings of transmembrane ion currents of a single channel embedded in artificial lipid bilayer membranes (BLMs), i.e., single-channel recordings, are useful for electrophysiological and kinetic studies of biological channels, and also for the development of channel-based sensors that afford amplified detection of analytes. 1,2The majority of single-channel recordings has been performed using planar BLMs with two separated aqueous phases, which were formed by a variety of techniques, such as painting, 3 monolayer folding 4,5 and tip-dip 6 methods. Planar BLMs have been utilized for the design of various kinds of electrochemical sensors. 1On the other hand, various supported bilayer membranes have been reported, including those formed on metals, 7 tethered ones formed on gold 8,9 and a peptide, 10 those formed on porous supports 11 and those on hydrogels, such as agarose 12-14 and agar. [15][16][17] These BLMs have attracted increasing interest due to their mechanical stability, which affords fabricating robust chemical sensors and molecular electronic devices.7 However, the metal-supported BLMs lack an aqueous phase on the metal support side, and hence ion translocation across the BLMs is not feasible, and the microscopically rough metal surface causes a local disturbance of the membrane structure, and consequently leaks. 17 The recordings of ion translocation so far reported as changes in the transmembrane ion currents [12][13][14][16][17][18] and the membrane potential, 15 have utilized mostly hydrogel-supported BLMs in which bioelements were embedded. However, few studies 13,14 have reported single-channel recordings, where a high electric resistance is essential for discriminating a singlechannel current at the level of a few pA from the background one. Ide et al. succeeded in making simultaneous optical and electric recording of single channels, such as a BK-channel 14 and alametithin, 13 using painted BLMs formed on agarosecoated glasses. For the design of channel-based sensing devices, excellent ion-blocking properties and a long lifetime along with an easy procedure for BLM formation become essential.In the present paper, we describe agarose-supported BLMs formed by the tip-dip and painting methods, which enable single-channel recordings of gramicidin. Gramicidin channels have been utilized for designing different types of channelbased BLM sensors. 8,9,19,20 The recording of a single-channel current is essential for the design of an ion-channel sensor. 19,21The properties of the supported BLMs are compared with those of unsupported ones in terms of a lifetime of the membranes, probability of single-channel recordings and open-close kinetics of gramicidin channels. Experimental MaterialsPhosphatidylcholine (PC, purity >99%, chloroform solution), phosphatidylethanolamine (PE, chloroform solution), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(2,4-dinitrophenyl) (DNP-PE) and 1,2-dipalmitoleoyl-sn-glycero-3-phosphocholine (DPOPC, >99%, chloroform solution) were purchased from Avanti Polar Lipids, Inc...

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Biosensors Based on Thin Lipid Films and Liposomes

Nikolelis¹,

Hianik

Krull³

1999

Electroanalysis

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This article reports the theory and analytical applications of thin lipid ®lms. Recent advances of electrochemical devices based on lipid membranes have lead to reports of construction of biosensors for environmental and food applications, and may provide opportunities for commercial fabrication. The methods of formation of lipid membranes on various supports including metals (silver, gold, stainless steel), agar, conducting polymers and ultra®ltration membranes have provided stabilization of lipid ®lms with a diversity of analytical applications in real samples. Methods of immobilization and incorporation of various functional macromolecules are summarized. Several examples of the application of various methods for study of physical properties of supported bilayer lipid membranes are described. Applications of lipid-based biosensors in analytical chemistry for determination of compounds are demonstrated, including a diversity of chemical compounds such as environmental pollutants (ammonia and carbon dioxide, cyanide ions, etc.) and food toxins (a¯atoxin M 1 and direct detection of toxin in real samples such as milk and milk preparations). Methods for application of liposomes as a sensing system are also summarized.

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Agar-supported lipid bilayers — basic structures for biosensor design. Electrical and mechanical properties

Cited by 28 publications

References 17 publications

Stable insulating tethered bilayer lipid membranes

Stable insulating tethered bilayer lipid membranes

Single-Channel Recordings of Gramicidin at Agarose-Supported Bilayer Lipid Membranes Formed by the Tip-Dip and Painting Methods

Biosensors Based on Thin Lipid Films and Liposomes

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