Agar Diffusion Method for Detection and Bioassay of Antiviral Antibiotics

Production of Potato Common Scab-antagonistic Biofertilizer from Swine Feces with Streptomyces albidoflavus

“…The antibiotic activity of the supernatant (0.05 ml), for 14 strains, was assayed by the paper-disc agar diffusion method. 8 ) Composting process for swine feces. The selected strain was inoculated onto a slant of swine feces extract agar medium and then incubated at 30°C for 5 days.…”

A simple plaque assay for influenza A2 viruses and its application to antiviral screening

“…Initially this plaque technique was used for screening the activity of antiviral agents by incorporating the test compound in a filter paper disc placed on the overlay surface and measuring the zone of plaque inhibition, as described by H~RRM~N~ et al (6). Although this method facilitated the screening of large numbers of compounds, it was found to be unreliable, probably due to inability of some compounds to diffuse through the overlay.…”

Cardinal Problems in Search for Antiviral Drugs

“…Also, so far, chemicals appear ed to be more successful than the agents from biological sources. On the other hand, it has been clearly indicated by many (4,5,6) that natural sources contain a wealth of diverse biochemicals, potentially useful as antiviral drugs.…”

“…This might seem to be a fairly adequate approach if it were not for the fact that the advent of a broad spectrum antiviral chemotherapeutic agent still seems to be remote and the balance of evidence is rather in favour of the develop ment of antiviral drugs with a narrow range of activity. Perhaps the maximum that could be reasonably expected from one chemical com pound is a breadth activity to embrace viruses with identical patterns of virus-cell interaction (2,4,11,12,13).…”