Ag(I)-cysteamine complex based electrochemical stripping immunoassay: Ultrasensitive human IgG detection

Noh, Hui-Bog; Rahman, Md. Aminur; Yang, Jee Eun; Shim, Yoon‐Bo

doi:10.1016/j.bios.2011.04.058

Cited by 28 publications

(12 citation statements)

References 35 publications

(34 reference statements)

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“…Thiophene-based oligomers and polymers have attracted considerable attention because of their unique π-conjugation which allows them to be utilized as building blocks of next-generation functional materials in environmental science, biological sensing technologies, and organic electronic technologies. − The majority of these applications involve the formation of interfaces between metals and thiophene-based molecules. Examples include uses such as terthiophene-functionalized Ag nanoparticles as H 2 O 2 sensors or for immunoassays in biological systems, use of oligothiophene-functionalized metal nanoparticles as “dendrons” to immobilize and stabilize dendrimers on highly ordered metal surfaces for energy storage applications, or employing oligo- or polythiophenes as active layers in organic photovoltaic devices in which thiophenes directly contact Al or Ag/Mg alloy electrodes. , Therefore, understanding the fundamental nature of the chemistry at oligothiophene/metal interface is essential to facilitate and improve materials design and applications at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Understanding the Reaction Chemistry of 2,2′:5′,2″-Terthiophene Films with Vapor-Deposited Ag, Al, and Ca

2015

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The reaction chemistry of vapor-deposited 2,2′:5′,2″-terthiophene (α-3T) solid-state thin films with postdeposited Ag, Al, and Ca is investigated in ultrahigh vacuum using Raman spectroscopy. Vapor-deposited Ag forms nanoparticles on these films and induces considerable surface enhanced Raman scattering (SERS) along with a change in molecular symmetry of adjacent α-3T and formation of Ag−S bonds; no other reaction chemistry is observed. Vapordeposited Al and Ca undergo chemical reaction with α-3T initiated by metal-to-α-3T electron transfer. For Al, the resulting product is predominantly amorphous carbon through initial radical formation and subsequent decomposition reactions. For Ca, the spectral evidence suggests two pathways: one leading to α-3T polymerization and the other resulting in thiophene ring opening, both initiated by radical formation through Ca-to-α-3T electron transfer. These interfacial reactions reflect the complex chemistry that can occur between low work function metals and thiophene-based oligomers. This reactivity is strongly correlated with metal work function.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Understanding the Reaction Chemistry of 2,2′:5′,2″-Terthiophene Films with Vapor-Deposited Ag, Al, and Ca

2015

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“…Following a similar protocol was studied a glucose sensor based on pTTP coated with AuZn oxide layer [ 536 ]. In another study, the fabrication of an ultrasensitive electrochemical immunosensor for detecting human immunoglobulin G (IgG) [ 537 ]. In here, the target protein IgG was sandwiched between the anti-IgG antibody, covalently attached to the pTTP via amide bond, and the Ag (I)-cysteamine complex (Ag–Cys) adsorbed onto gold nanoparticles (AuNPs)–anti-IgG.…”

Section: Bioapplicationsmentioning

confidence: 99%

Thiophene-Based Trimers and Their Bioapplications: An Overview

et al. 2021

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Certainly, the success of polythiophenes is due in the first place to their outstanding electronic properties and superior processability. Nevertheless, there are additional reasons that contribute to arouse the scientific interest around these materials. Among these, the large variety of chemical modifications that is possible to perform on the thiophene ring is a precious aspect. In particular, a turning point was marked by the diffusion of synthetic strategies for the preparation of terthiophenes: the vast richness of approaches today available for the easy customization of these structures allows the finetuning of their chemical, physical, and optical properties. Therefore, terthiophene derivatives have become an extremely versatile class of compounds both for direct application or for the preparation of electronic functional polymers. Moreover, their biocompatibility and ease of functionalization make them appealing for biology and medical research, as it testifies to the blossoming of studies in these fields in which they are involved. It is thus with the willingness to guide the reader through all the possibilities offered by these structures that this review elucidates the synthetic methods and describes the full chemical variety of terthiophenes and their derivatives. In the final part, an in-depth presentation of their numerous bioapplications intends to provide a complete picture of the state of the art.

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“…The sensor also offers low limit of detection of 0.15 ng mL À1 , limit of quantitation of 0.50 ng mL À1 and high sensitivity (11.297 lA cm À2 ), which are sufficient for the detection of immunoglobulin G at the physiological level. Table 1 shows the detection performances for detecting immunoglobulin G using our proposed immunosensor as compared to other immunosensors reported in the literatures [32][33][34][35][36][37][38][39]. Entries 1-4 are of sandwich structured immunosensors whilst entries 5-8 are of label-free immunosensors.…”

Section: Linear Response and Limit Detection Of The Immunosensormentioning

confidence: 99%

A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G

Putnin

Jumpathong

Laocharoensuk

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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This work focuses on fabricating poly(2-aminobenzylamine)-modified screen-printed carbon electrode as an electrochemical immunosensor for the label-free detection of human immunoglobulin G. To selectively detect immunoglobulin G, the anti-immunoglobulin G antibody with high affinity to immunoglobulin G was covalently linked with the amine group of poly(2-aminobenzylamine) film-deposited screen-printed carbon electrode. The selectivity for immunoglobulin G was subsequently assured by being challenged with redox-active interferences and adventitious adsorption did not significantly interfere the analyte signal. To obviate the use of costly secondary antibody, the [Fe(CN)] redox probe was instead applied to measure the number of human immunoglobulin G through the immunocomplex formation that is quantitatively related to the level of the differential pulse voltammetric current. The resulting immunosensor exhibited good sensitivity with the detection limit of 0.15 ng mL, limit of quantitation of 0.50 ng mL and the linear range from 1.0 to 50 ng mL. Given those striking analytical performances and the affordability arising from using cheap screen-printed carbon electrode with label-free detection, the immunosensor serves as a promising model for the next-step development of a diagnostic tool.

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Ag(I)-cysteamine complex based electrochemical stripping immunoassay: Ultrasensitive human IgG detection

Cited by 28 publications

References 35 publications

Understanding the Reaction Chemistry of 2,2′:5′,2″-Terthiophene Films with Vapor-Deposited Ag, Al, and Ca

Understanding the Reaction Chemistry of 2,2′:5′,2″-Terthiophene Films with Vapor-Deposited Ag, Al, and Ca

Thiophene-Based Trimers and Their Bioapplications: An Overview

A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G

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