AFLP analysis of somaclonal variation in Arabidopsis thaliana regenerated plants

Polanco, Carlos; Ruiz, María Luisa Sanchiz

doi:10.1016/s0168-9452(02)00029-8

Cited by 78 publications

(55 citation statements)

References 39 publications

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“…Although AFLP analysis is not as widely used in this type of study as RFLP or RAPD, Polanco and Ruiz (2002) used it to detected somaclonal variation in Arabidopsis and Vendrame et al (1999) in pecan nuts. In asparagus, Raimondi et al (2001) found no intraclonal polymorphisms for RAPD in 77 regenerants obtained by somatic embryogenesis from the same clones as were used in our study, but Raimondi et al (2001) used a smaller number of markers (157 as against 408 in our study) and, analogously to what was previously discussed regarding ploidy variation, differences in callus age and/or mode of regeneration of somaclones could account for the differences in the results.…”

Section: Discussionmentioning

confidence: 99%

Somaclonal variation in Asparagus officinalis plants regenerated by organogenesis from long-term callus cultures

Pontaroli

Camadro

2005

Genet. Mol. Biol.

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Somaclonal variation in plants regenerated by organogenesis from long-term cultured calluses of two diploid staminate genotypes of Asparagus officinalis cv. Argenteuil was characterized by plant phenotype, ploidy, meiotic behavior, pollen viability, fruit and seed set, and AFLP profiles. Phenotypic deviations from the donors were detected in foliage color, flower size, and cladode and flower morphology. Ploidy changes were observed in 37.8% of the 37 regenerants studied. Meiotic alterations in 12 out of 21 regenerants included laggards, dicentric bridges, micronuclei, restitution nuclei and polyads. Of the 408 AFLP markers screened in 43 regenerants and the donors, 2.94% showed polymorphism. High pollen viability was observed in the 22 regenerants analyzed. All crosses between one pistillate plant and 35 regenerants, as well as the controls, produced fruits and seeds; however, no plump seeds resulted in 35.3% of the crosses with regenerants, and no seeds germinated in 12.5% of those with apparently normal seeds. Fruit and seed set was similar in crosses with diploid regenerants with normal meiosis and the controls but was lower in crosses with diploid and polyploid regenerants with abnormal meiosis. Our results show that the regenerated plants exhibited conspicuous somaclonal variation that could be eventually exploited for in vitro selection systems.

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Section: Discussionmentioning

confidence: 99%

Somaclonal variation in Asparagus officinalis plants regenerated by organogenesis from long-term callus cultures

Pontaroli

Camadro

2005

Genet. Mol. Biol.

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“…However, when setting up a protocol to regenerate plantlets for industrial and commercial purposes, the primary goal is to keep specific desirable characteristics of the plant unaltered. Since tissue culture-induced variations can yield unwelcome phenomena, it is important to know the frequency, the genomic distribution, the mechanisms and the factors influencing those characteristics [4]. Somaclonal variation can be analysed at both the phenotypic and genotypic levels.…”

Section: Introductionmentioning

confidence: 99%

“…The RFLP technique requires large amounts of high quality DNA, and probe availability is also an issue. The RAPD technique has proven inconclusive in many cases due to its low reproducibility and its low potential of revealing tissue culture-induced variations [4]. By contrast, the AFLP technique allows the performance of reproducible analyses with reduced amounts of DNA, combining the peculiarities of RFLPs and PCR-based protocols and revealing a greater number of polymorphisms.…”

Section: Introductionmentioning

confidence: 99%

“…By contrast, the AFLP technique allows the performance of reproducible analyses with reduced amounts of DNA, combining the peculiarities of RFLPs and PCR-based protocols and revealing a greater number of polymorphisms. This has made AFLP a powerful tool that has been employed in several studies of somaclonal variation: in oak [8], pecan trees [9], sugarcane [10], sunflower [11], and Arabidopsis thaliana [4]. Some authors [12,13] compared the different types of molecular marker classes for discovering plant genetic variation, and all stated that AFLP consistently showed higher efficiency in detecting polymorphisms than RFLP, RAPD and SSR.…”

Section: Introductionmentioning

confidence: 99%

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An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Perrini

Alba

Ruta

et al. 2009

Cellular and Molecular Biology Letters

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A protocol for the induction of regeneration from leaves of Helichrysum italicum was established. Calli were found to form on the basal medium only when it was supplemented with thidiazuron (TDZ) alone or in combination with naphthalene acetic acid (NAA), with a percentage ranking of at least 80%. The hormone-free medium showed the highest percentage of shoot regeneration (62%) even though no callus formed. AFLP markers were employed to verify tissue culture-induced variation in the regenerated plantlets obtained by direct shoot regeneration or the indirect shoot regeneration process (callus formation). Seven out of the eleven AFLP primer pairs yielded polymorphic patterns. The average number of fragments per primer pair was 64.1. Singletons were represented by 12 (2.7%) fragments. Student’s T-test was performed both on the average number of shared fragments and on the nucleotide diversity, and no significant statistical difference was observed between the two regeneration treatments.

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“…AFLP have been used to detect somaclonal variation in plants generated in vitro in Carya illinoinensis (Vendrame et al, 1999), Arabidopsis thaliana (Polanco and Ruiz, 2002), Alnus acuminata H.B.K. (Gutíerrez, 2002;Marulanda et al, 2006), Agave fourcroydes (González et al, 2004), Hevea brasiliensis (Medina et al, 2007) and Manihot esculenta (Medero et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Variabilidad genética de cultivo in vitro de aguacate raza mexicana

Cerda-Hurtado

Ojeda-Zacarías

Iracheta-Donjuan

et al. 2018

Remexca

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ResumenExplantes foliares de Persea americana Mill. var. drymifolia, se establecieron en medio DCR (Gupta y Durzan, 1985) suplementado con vitaminas y reguladores del crecimiento para producir callos, dos sub cultivos se realizaron en intervalos de 30 a 45 días entre ellos. Con el fin de determinar la variabilidad genética se generaron 341 marcadores AFLP (amplified fragment lenght polymorphism) polimórficos con 10 combinaciones de iniciadores, se analizaron 94 muestras: la planta madre, 31 callos, 31 callos del primer sub cultivo y 31 callos del segundo sub cultivo. Se determinó el contenido de información polimórfica (PIC) por combinación de iniciadores y se calculó la distancia genética entre muestras para hacer el análisis de agrupamiento. Se obtuvo la diversidad genética entre la planta madre, callos y callos de los dos sub cultivos y se realizó ei análisis de varianza molecular. Los valores PIC indicaron que las combinaciones de iniciadores fueron adecuadas para estimar la variabilidad genética de los callos. En el análisis de agrupamiento, la planta madre mostró distancias de 0.63 a 0.7 con respecto a sus callos, distancias de 0.78-0.99 se obtuvieron entre los callos y los callos de los dos sub cultivos. La diversidad genética presentó diferencias significativas, el análisis de varianza molecular demostró variabilidad genética entre AbstractLeaf explants of Persea americana Mill. var. drymifolia were established on DCR medium (Gupta and Durzan, 1985) supplemented with vitamins and growth regulators to produce callus, two sub cultures were made in intervals of 30 to 45 days between them. In order to determine the genetic variability 341 AFLP (amplified fragment lenght polymorphism) polymorphism with 10 primer combinations were generated, 94 samples were analyzed: the mother plant, 31 calluses, 31 calluses from the first sub culture and 31 calluses from the second sub culture. Polymorphic information content (PIC) was determined by primer combination and the genetic distance between samples was calculated for the cluster analysis. Genetic diversity between the mother plant, calluses and calluses of the two sub cultures were obtained and the analysis of molecular variance was performed. The PIC values indicated that the primer combinations were adequate to estimate the genetic variability of calluses. In the cluster analysis, the mother plant showed distances of 0.63 to 0.7 in regard to their calluses, distances of 0.78 -0.99 were obtained between calluses and calluses of the two sub cultures. Genetic diversity showed significant differences, analysis of molecular variance demonstrated genetic variability between the mother plant, calluses and calluses of the two sub cultures IntroducciónEn plantas el cultivo in vitro es una alternativa para la conservación ex situ, el intercambio y conservación de germoplasma y la propagación clonal; la selección del explante depende de los recursos y objetivos finales de la técnica. Diversos explantes se han utilizado para obtener tipos diferentes de morfogénesis en agu...

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AFLP analysis of somaclonal variation in Arabidopsis thaliana regenerated plants

Cited by 78 publications

References 39 publications

Somaclonal variation in Asparagus officinalis plants regenerated by organogenesis from long-term callus cultures

Somaclonal variation in Asparagus officinalis plants regenerated by organogenesis from long-term callus cultures

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Variabilidad genética de cultivo in vitro de aguacate raza mexicana

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