2017
DOI: 10.1080/19420862.2017.1323159
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Abstract: Human IgG is the main antibody class used in antibody therapies because of its efficacy and longer halflife, which are completely or partly due to FcgR-mediated functions of the molecules. Preclinical testing in mouse models are frequently performed using human IgG, but no detailed information on binding of human IgG to mouse FcgRs is available. The orthologous mouse and human FcgRs share roughly 60-70% identity, suggesting some incompatibility. Here, we report binding affinities of all mouse and human IgG sub… Show more

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Cited by 180 publications
(147 citation statements)
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“…4B). The obtained affinities of mIgG1, mIgG2a and mIgG2b for the FcγRs fall well within the range of previously reported K D values for recombinantly produced isotypes (Table S3) (26). In summary, mIgG1 did not bind mFcγRI and mFcγRIV, but binds to mFcγRIIb;…”
Section: Biochemical and Functional Characterization Of The Engineeresupporting
confidence: 88%
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“…4B). The obtained affinities of mIgG1, mIgG2a and mIgG2b for the FcγRs fall well within the range of previously reported K D values for recombinantly produced isotypes (Table S3) (26). In summary, mIgG1 did not bind mFcγRI and mFcγRIV, but binds to mFcγRIIb;…”
Section: Biochemical and Functional Characterization Of The Engineeresupporting
confidence: 88%
“…4A) and generated affinity plots to calculate the dissociation constants (K D ) for each receptor concentration. Subsequently, these were interpolated to calculate the affinity of each antibody at receptor concentration giving R max =500, as described before (26) (Fig. 4B).…”
Section: Biochemical and Functional Characterization Of The Engineerementioning
confidence: 99%
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“…40 It has been previously reported in NSG mice, that CART cells expressing an IgG Fc domain-based CAR underwent interactions with Fc gamma receptor (FcγR)-expressing myeloid cells, which led to a tumor-independent trapping of CART cells in the lung, excessive T cell activation and, consequently, lack of in vivo efficacy. [40][41][42] Thus, since the CD19-specific CAR used in this study also contains an IgG Fc spacer, the observed failure of full cancer eradication and the lack of circulating CART cells in the murine PB 8 days after infusion is most likely caused by myeloid cell-mediated activation-induced cell death (AICD) of CART cells in NSG mice due to lacking circulating IgG that normally shield FcγRI (data not shown). 40 Administration of murine or human IgG before CART cell transfer could avoid this off-target activation of CART cells and improve persistence in NSG mice.…”
Section: Discussionmentioning
confidence: 95%
“…Mouse IgG2a antibody is considered as a functional equivalent of human IgG1 antibodies, which constitute the most abundant among all serum IgG isotypes. 9,10 Immunization of human infants with pneumococcal conjugate vaccines has been reported to result in rises in IgG1 antibodies and opsonophagocytosis of antibodies to S. pneumoniae capsular polysaccharides. 11 In line with these findings, our current study has shown a significant increase in S. mitis-specific IgG2a antibodies reactive to S. pneumoniae serotypes 2 and 4, which hints at the implication of this antibody isotype in S. mitis mediated protective immunity to S. pneumoniae.…”
Section: Discussionmentioning
confidence: 99%