Affinity hemosorbents based on aromatic peptides for the binding of immunoglobulins G

Abstract

Affinity haemoadsorbents based on WY, WTY, WNY ligands and polysaccharide matrix were developed for the human immunoglobulin G binding. The characteristics of new sorbents such as the binding of total IgG and binding of IgG subclasses were compared. It was found that all new sorbents well extract the IgG from the blood plasma. It was evidenced that WNY-based sorbent is more effective for binding of IgG subclass 3. The determination of physic-chemical characteristics of IgG binding revealed that desorption constants for IgG are 10 ± 3, 28 ± 4 and 13 ± 3 µM for WY, WTY, WNY based sorbents respectively. Maximum sorption capacities for IgG are 43 ± 2, 45 ± 3 and 46 ± 3 mg IgG per ml of sorbent for WY, WTY, WNY based sorbents respectively. Also it was shown that the new sorbents are compatible with blood and are suitable for the medical purposes.

A Sorbent with Synthetic Ligand for Removing Pro-atherogenic and Pro-inflammatory Components from Human Blood Plasma

“…A comparative analysis was performed using immune sorbents with immobilized polyclonal antibodies against LDL (LDL–Lipopak®), against IgG (Ig Adsopak®, both LTD sorbents manufactured by POCARD, Russia), and against nCRP. The sorbent with a synthetic ligand was obtained by immobilization of aromatic aldehyde on a cross-linked agarose matrix using a molecular spacer according to the method described earlier [ 17 ], albeit modified. The synthesis was carried out without glutaraldehyde.…”

Covalently immobilized chemically modified lysozyme as a sorbent for bacterial endotoxins (lipopolysaccharides)

“…In this work we set a goal to determine the resulting degree of modification (the number of modified amino groups per enzyme molecule) at various modifier/protein molar ratios in the reaction mixture, compare bacteriolytic properties of native and modified lysozyme, then obtain covalently immobilized lysozyme with different degrees of chemical modification and study bacteriolytic activity and sorption characteristics of new materials. As a polymer agarose matrix for lysozyme immobilization we chose WorkBeads 200SEC with a granule diameter of 200 μm for which its hemocompatibility and suitability for use in medicine in extracorporeal blood purification procedures was previously shown [3,6,14]. To determine the bacteriolytic activity we chose gram-negative Escherichia coli and gram-positive Micrococcus luteus cells as model bacteria substrates.…”

Сравнительная характеристика сорбентов для удаления иммуноглобулинов (сравнение in vitro)

“…В клинической практике для удаления аутоантител помимо плазмафереза используются сорбенты с иммобилизованными поликлональными Ат к Ig человека [9,11,12], стафилококковым белком А [13], а также синтетическими лигандами -пептидом PGam146 [14] или триптофаном [15]. Ранее мы синтезировали сорбент с иммобилизованным на макрогранулированную матрицу синтетическим лигандом, пригодный для перфузии крови [16]. Использование такого сорбента существенно упрощает процедуру афереза и позволяет расширить сферу его применения.…”