Aeromonas veronii tolC modulates its virulence and the immune response of freshwater pearl mussels, Hyriopsis cumingii

Zheng, Sichun; Tang, Xiaoqi; Yang, Qinglin; Zhou, Xicheng; Li, Yanhong; Wu, Zhengli

doi:10.1016/j.dci.2024.105137

Cited by 2 publications

(3 citation statements)

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“…Researchers prepared a live attenuated A.veronii vaccine by suppressing the expression of SmpB, to immunize Zebrafish , and achieved a survival rate of 65% after challenge with a virulent strain ( 13 ). A tolC gene-deleted live attenuated vaccine significantly reduced tissue damage in Hyriopsis cumingii infected by a virulent strain ( 14 ). An hcp gene-deleted attenuated vaccine immunized the largemouth bass, achieving a 100% survival rate, significantly higher than the 76.67% survival rate of the formalin inactivated vaccine immunization group ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

“…The administration routes of fish vaccine include injection, oral, and immersion. Injection immunization includes muscle injection and intraperitoneal injection ( 14 , 18 ). The site for muscle injection is generally the muscle at the base of the dorsal fin or tail fin of the fish, while the intraperitoneal injection site is often inside the peritoneum at the base of the ventral fin.…”

Section: Introductionmentioning

confidence: 99%

“…The safety of the live attenuated vaccines raises concerns, particularly regarding their potential reversion to virulence or adverse effects in immunocompromised individuals. And, the precise duration of immunity these vaccines confer remains elusive, prompting inquiries into the necessity for booster shots and sustained protection for vaccinated groups (13)(14)(15)17). Therefore, while A.veronii vaccine development is a promising strategy for infection control, it necessitates ongoing research to address these prevailing issues.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the efficacy of Aeromonas veronii ΔhisJ vaccine in Carassius auratus via different immunization routes

Wu,

Ma,

Pan

et al. 2024

Front. Vet. Sci.

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IntrodctionAeromonas veronii is a significant pathogen to various aquatic life. Infections in fish can lead to high mortality rates, causing substantial economic losses in aquaculture. Vaccination is proposed as a substitute for antibiotics in aquaculture to decrease disease-related mortality and morbidity. Our study previously constructed a hisJ-deleted strain of A. veronii, which provided protective effect to Loach.MethodsTo further assess the vaccine’s applicability, this study evaluated its genetic stability and safety, and the immune protective effects in Carassius auratus through four distinct administration routes: intraperitoneal injection, intramuscular injection, oral administration, and immersion, to determine the efficacy of these administration routes.ResultsThe results showed that the vaccine remained genetically stable after 45 generations. Immunization via these administration routes was safe for Carassius auratus, with intraperitoneal and intramuscular injections causing stronger adverse reactions. Immersion immunization resulted in mild adverse reactions, and no significant adverse reactions were observed following oral immunization. Immunizing Carassius auratus at safe concentrations via these routes enhanced the phagocytic activity in serum, increased the levels of non-specific immune-related enzymes (ACP, AKP, C3, C4, LZM, SOD, and IgM), and improved specific serum antibody levels. It also elevated levels of cytokines related to inflammatory responses (IL-1β, IL-10, TNF-α, TGF-β) in organ tissues (liver, spleen, kidney, mid-post intestine, and gills). The survival rates of Carassius auratus were measured after challenging with the virulent strain A. veronii TH0426, resulting in the relative survival rates of 64% for Intraperitoneal vaccine group, 56% for Intramuscular vaccine group, 52% for oral vaccine group, and 48% for immersion vaccine group. Analysis of bacterial load in the liver, spleen, and kidney post-challenge showed a decreasing trend in the control group, indicating that the vaccine strain ΔhisJ could gradually restrict the rapid proliferation of bacteria in these tissues, thereby providing a certain level of immune protection against A. veronii.DiscussionIn brief, the vaccine strain ΔhisJ can serve as a safe live attenuated vaccine for Carassius auratus, and this study lays the foundation for the development of live attenuated vaccines against Aeromonas veronii.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%