Aeromonas dhakensis is not a rare cause of Aeromonas bacteremia in Hiroshima, Japan

Kitagawa, Hirohisa; Ohge, Hiroki; Yu, Liansheng; Kayama, Shizuo; Hara, Takatoshi; Kashiyama, Seiya; Kajihara, Toshiki; Sueda, Taijiro; Sugai, Motoyuki

doi:10.1016/j.jiac.2019.08.020

Cited by 30 publications

(26 citation statements)

References 13 publications

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“…40 out of 58 patients were male, which may attribute to that alcoholic cirrhosis was more prevalent in male than female in our study. Similar to previous researches [ 20 ], we also found that the majority of patients had a variety of underlying diseases, including liver cirrhosis, diabetes mellitus, under immunosuppressed conditions, leukemia and other kinds of malignancy. Nearly half of patients in this study were diagnosed with liver cirrhosis.…”

Section: Discussionsupporting

confidence: 89%

Taxonomy, virulence determinants and antimicrobial susceptibility of Aeromonas spp. isolated from bacteremia in southeastern China

Sun

Zhao

et al. 2021

Antimicrob Resist Infect Control

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Background The study aimed to elucidate the species taxonomy, clinical manifestations, virulence gene profiles and antimicrobial susceptibilities of Aeromonas strains isolated from life-threatening bacteremia in southeastern China. Methods Clinical samples of Aeromonas causing bacteremia were isolated from a teaching hospital in Wenzhou from 2013 to 2018 and a retrospective cohort study was performed. Aeromonas strains were identified at species level by housekeeping gene gyrB. Virulence and drug resistance-associated genes were screened by polymerase chain reaction (PCR) and antimicrobial susceptibility testing (AST) was performed by the VITEK 2 Compact system. Results A total of 58 Aeromonas isolated from patients with bacteremia were collected during 6 years (2013–2018). 58 isolates were identified to five different species, where Aeromonas dhakensis appeared to be the predominant species (26/58), followed by Aeromonas veronii (13/58), Aeromonas caviae (10/58), Aeromonas hydrophila (7/58) and Aeromonas jandaei (2/58). 16 of 58 patients had poor prognosis. Poor prognosis was significantly associated with liver cirrhosis and inappropriate empirical antimicrobials therapy. The progression of bacteremia caused by Aeromonas was extremely fast, especially in A. dhakensis infections. Virulence genes aer, lip, hlyA, alt, ast, and act, were detected at ratios of 24.1% (14/58), 62.1% (36/58), 65.5% (38/58), 58.6% (34/58), 15.5% (9/58) and 65.5% (38/58), respectively. Antimicrobial susceptibility testing exhibited that 9 out of 58 isolates were identified as multi-drug resistant (MDR) organism. The blaTEM gene was identified in all 9 MDR isolates. blaSHV, blaAQU-1, blaMOX, blaCepH, blaCphA and aac(6′)-Ib-cr were detected in 4 isolates, 2 isolates, 1 isolate, 3 isolates, 8 isolates, and 3 isolates, respectively. The majority of Aeromonas strains maintained susceptible to 3rd generation cephalosporins, aminoglycosides, fluoroquinolones and furantoin. Conclusions The prevalence and dangerousness of Aeromonas infections, especially A. dhakensis, are underestimated in clinic. Continuous monitoring is essential to keep track of MDR Aeromonas due to the increasing prevalence recently and a more effective measure is required to control the spread of resistance determinants.

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Section: Discussionsupporting

confidence: 89%

Taxonomy, virulence determinants and antimicrobial susceptibility of Aeromonas spp. isolated from bacteremia in southeastern China

Sun

Zhao

et al. 2021

Antimicrob Resist Infect Control

View full text Add to dashboard Cite

show abstract

“…40 out of 58 patients were male, which may attribute to that alcoholic cirrhosis was more prevalent in male than female in our study. Similar with previous researches [15] that the majority of patients had a variety of underlying diseases, including liver cirrhosis, diabetes mellitus, under immunosuppressed conditions, leukemia and other kinds of malignancy. Nearly half of patients in this study were diagnosed as liver cirrhosis.…”

Section: Discussionsupporting

confidence: 87%

Taxonomy, virulence determinants and antimicrobial susceptibility of Aeromonas spp. isolated from bacteremia in southeastern China

Sun

Zhao

et al. 2020

Preprint

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Background: The study was aimed to elucidate the species taxonomy, clinical manifestations, virulence gene profiles and antimicrobial susceptibilities of Aeromonas strains isolated from life-threatening bacteremia in southeastern China. Methods: Clinical samples of Aeromonas causing bacteremia were isolated from a teaching hospital in Wenzhou during 2013 to 2018 and retrospective cohort study was performed. Aeromonas strains were identified to species level by housekeeping gene gyrB. Six virulence-associated genes (aer, lip, hlyA, alt, ast, and act) were screened by polymerase chain reaction (PCR) and antibiotics susceptibility testing (AST) was performed by VITEK 2 Compact system.Results: A total of 58 patients with bacteremia caused by Aeromonas species were collected during 6 years (2013-2018). 58 isolates were identified to five different species, where Aeromonas dhakensis appeared to be predominant (26/58), followed by Aeromonas veronii (13/58), Aeromonas caviae (10/58), Aeromonas hydrophila (7/58) and Aeromonas jandaei (2/58). 16 of 58 patients had poor prognosis. Poor prognosis was significantly associated with community-acquired infections and liver cirrhosis. The progression of bacteremia caused by Aeromonas was extremely fast, especially in A. dhakensis infections. Virulence genes aer, lip, hlyA, alt, ast, and act, were detected at ratios of 24.1% (14/58), 62.1% (36/58), 65.5% (38/58), 58.6% (34/58), 15.5% (9/58) and 65.5% (38/58), respectively. Antimicrobials susceptibility exhibited that 9 out of 58 isolates were identified as multi-drug resistant (MDR) organism. The majority of Aeromonas strains maintained susceptible to 3rd generation cephalosporins, aminoglycosides, fluoroquinolones and furantoin. Conclusions: The prevalence and dangerousness of Aeromonas infections, especially A. dhakensis, are underestimated in clinic. Continuous monitoring is essential to keep track of MDR Aeromonas due to the increasing prevalence recently and more effective measure is required to control the spread of resistance determinants.

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“…Aeromonas spp. are known to carry several chromosomal β-lactam resistance genes belonging to Ambler classes B, C and D [18,20,[22][23][24]. By obtaining the genetic sequences of these β-lactamase genes through WGS, we hoped to shed light on whether the class C cephalosporinases MOX and FOX, now pervasive among the Enterobacteriaceae, originate from the Aeromonas genus.…”

Section: Discussionmentioning

confidence: 99%

Taxonomic Identification of Different Species of the Genus Aeromonas by Whole-Genome Sequencing and Use of Their Species-Specific β-Lactamases as Phylogenetic Markers

et al. 2021

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Some Aeromonas species, potentially pathogenic for humans, are known to express up to three different classes of chromosomal β-lactamases, which may become hyperproduced and cause treatment failure. The aim of this study was to assess the utility of these species-specific β-lactamase genes as phylogenetic markers using whole-genome sequencing data. Core-genome alignments were generated for 36 Aeromonas genomes from seven different species and scanned for antimicrobial resistance genes. Core-genome alignment confirmed the MALDI-TOF identification of most of the isolates and re-identified an A. hydrophila isolate as A. dhakensis. Three (B, C and D) of the four Ambler classes of β-lactamase genes were found in A. sobria, A. allosacharophila, A. hydrophila and A. dhakensis (blaCphA, blaAmpC and blaOXA). A. veronii only showed class-B- and class-D-like matches (blaCphA and blaOXA), whereas those for A. media, A. rivipollensis and A. caviae were class C and D (blaCMY, blaMOX and blaOXA427). The phylogenetic tree derived from concatenated sequences of β-lactamase genes successfully clustered each species. Some isolates also had resistance to sulfonamides, quinolones and aminoglycosides. Whole-genome sequencing proved to be a useful method to identify Aeromonas at the species level, which led to the unexpected identification of A. dhakensis and A.rivipollensis and revealed the resistome of each isolate.

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Aeromonas dhakensis is not a rare cause of Aeromonas bacteremia in Hiroshima, Japan

Cited by 30 publications

References 13 publications

Taxonomy, virulence determinants and antimicrobial susceptibility of Aeromonas spp. isolated from bacteremia in southeastern China

Taxonomy, virulence determinants and antimicrobial susceptibility of Aeromonas spp. isolated from bacteremia in southeastern China

Taxonomy, virulence determinants and antimicrobial susceptibility of Aeromonas spp. isolated from bacteremia in southeastern China

Taxonomic Identification of Different Species of the Genus Aeromonas by Whole-Genome Sequencing and Use of Their Species-Specific β-Lactamases as Phylogenetic Markers

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