Aedes nigrinus (Eckstein, 1918) (Diptera, Culicidae), a new country record for England, contrasted with Aedes sticticus (Meigen, 1838)

Harbach, Ralph E.; Dallimore, Thom; Briscoe, Andrew G.; Culverwell, C. Lorna; Vaux, Alexander G.C.; Medlock, Jolyon M.

doi:10.3897/zookeys.671.12447

Cited by 10 publications

(9 citation statements)

References 16 publications

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“…We followed the classification of Wilkerson et al (2015) for taxa in Aedini. The subgeneric placement for all species can be found in Harbach (2017) and Harbach et al (2017).…”

Section: Methodsmentioning

confidence: 99%

“…Nonetheless, these findings demonstrated that the UK is at risk of introduction by invasive species of Aedes (Dallimore et al, 2017). In addition, Harbach et al (2017) discovered Ae.nigrinus (Eckstein, 1918) in the New Forest, southern England, which brings the total count of named species to 37 [35 native species plus two records of invasive species]. In addition, the occurrence of certain species has been very sporadic as in the case of Ae.vexans (Meigen, 1830); however, Medlock et al (2017b) reported a resident population of this species at Marston Marshes, Norwich.…”

Section: Introductionmentioning

confidence: 99%

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DNA barcoding of British mosquitoes (Diptera, Culicidae) to support species identification, discovery of cryptic genetic diversity and monitoring invasive species

Hernández‐Triana¹,

Brugman²,

Nikolova³

et al. 2019

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Correct mosquito species identification is essential for mosquito and disease control programs. However, this is complicated by the difficulties in morphologically identifying some mosquito species. In this study, variation of a partial sequence of the cytochromecoxidase unit I (COI) gene was used for the molecular identification of British mosquito species and to facilitate the discovery of cryptic diversity, and monitoring invasive species. Three DNA extraction methods were compared to obtain DNA barcodes from adult specimens. In total, we analyzed 42 species belonging to the generaAedesMeigen, 1818 (21 species),AnophelesMeigen, 1818 (7 species),CoquillettidiaTheobald, 1904 (1 species),CulexLinnaeus, 1758 (6 species),CulisetaFelt, 1904 (7 species), andOrthopodomyiaTheobald, 1904 (1 species). Intraspecific genetic divergence ranged from 0% to 5.4%, while higher interspecific divergences were identified betweenAedesgeminusPeus, 1971/Culisetalitorea(Shute, 1928) (24.6%) andAe.geminus/An.plumbeusStephens, 1828 (22.5%). Taxonomic discrepancy was shown betweenAn.daciaeLinton, Nicolescu & Harbach, 2004 andAn.messeaeFalleroni, 1828 indicating the poor resolution of theCOIDNA barcoding region in separating these taxa. Other species such asAe.cantans(Meigen, 1818)/Ae.annulipes(Meigen, 1830) showed similar discrepancies indicating some limitation of this genetic marker to identify certain mosquito species. The combination of morphology and DNA barcoding is an effective approach for the identification of British mosquitoes, for invasive mosquitoes posing a threat to the UK, and for the detection of hidden diversity within species groups.

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“…We followed the classification of Wilkerson et al (2015) for taxa in Aedini. The subgeneric placement for all species can be found in Harbach (2017) and Harbach et al (2017).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

DNA barcoding of British mosquitoes (Diptera, Culicidae) to support species identification, discovery of cryptic genetic diversity and monitoring invasive species

Hernández‐Triana¹,

Brugman²,

Nikolova³

et al. 2019

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“…There have been 36 mosquito species reported in the UK [ 16 ] of which 15 are Aedinae and 4 Culex . Based on records of abundance and feeding preference the following have potential as RVFV vectors: Ae.…”

Section: Introductionmentioning

confidence: 99%

Competence of mosquitoes native to the United Kingdom to support replication and transmission of Rift Valley fever virus

et al. 2018

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BackgroundRift Valley fever phlebovirus (RVFV) is a mosquito-borne arbovirus causing severe disease in humans and livestock. It is endemic in Africa and spread to the Arabian Peninsula in 2000 raising concerns it could emerge in Europe. The ability of temperate mosquitoes from the United Kingdom (UK) to support replication and transmission of RVFV is unknown.MethodsIn this study, two colonised lines of Culex pipiens, wild-caught Aedes detritus and Ae. rusticus from the UK were infected with pathogenic strains of RVFV to assess their vector competence. Mosquitoes were offered artificial blood-meals containing 106 or 107 plaque forming units (PFU)/ml RVFV, simulating natural peak viraemia in young ruminants, and maintained at 20 °C or 25 °C for up to 21 days. Bodies, legs and saliva were collected and tested for the presence of viral RNA and infectious virus to determine the infection, dissemination and transmission potential.ResultsAcross temperatures, doses and strains the average infection, dissemination and transmission rates were: 35, 13 and 5% (n = 91) for Cx. pipiens (Caldbeck); 23, 14 and 5% (n = 138) for Cx. pipiens (Brookwood); 36, 28 and 7% (n = 118) for Ae. detritus. However, despite 35% (n = 20) being susceptible to infection, Ae. rusticus did not transmit RVFV. Survival of Aedes species was negatively affected by maintenance at 25 °C compared to the more representative peak average British summer temperature of 20 °C. Increased mortality was also observed with some species infected with 107 PFU/ml compared to 106 PFU/ml.ConclusionsIt can be concluded that temperate mosquito species present in the UK demonstrate a transmission potential for RVFV in the laboratory but, even at high temperatures, this occurred at low efficiency.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-2884-7) contains supplementary material, which is available to authorized users.

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“…DNA was isolated from individual eggs homogenized by electronic pestle, extracted using the DNeasy® Blood and Tissue Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer's instructions and amplified by polymerase chain reaction following the methods described in Harbach et al . (). Sequencing was carried out using an AB3500 genetic analyser (Applied Biosystems, Inc., Foster City, CA, U.S.A.).…”

Section: Methodsmentioning

confidence: 97%

The challenge of invasive mosquito vectors in the U.K. during 2016–2018: a summary of the surveillance and control of Aedes albopictus

Vaux

Dallimore

Cull

et al. 2019

Medical Vet Entomology

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Mosquito‐borne diseases resulting from the expansion of two key vectors, Aedes aegypti and Aedes albopictus (Diptera: Culicidae), continue to challenge whole regions and continents around the globe. In recent years there have been human cases of disease associated with Chikungunya, dengue and Zika viruses. In Europe, the expansion of Ae. albopictus has resulted in local transmission of Chikungunya and dengue viruses. This paper considers the risk that Ae. aegypti and Ae. albopictus represent for the U.K. and details the results of mosquito surveillance activities. Surveillance was conducted at 34 points of entry, 12 sites serving vehicular traffic and two sites of used tyre importers. The most common native mosquito recorded was Culex pipiens s.l. (Diptera: Culicidae). The invasive mosquito Ae. albopictus was detected on three occasions in southern England (September 2016, July 2017 and July 2018) and subsequent control strategies were conducted. These latest surveillance results demonstrate ongoing incursions of Ae. albopictus into the U.K. via ground vehicular traffic, which can be expected to continue and increase as populations in nearby countries expand, particularly in France, which is the main source of ex‐continental traffic.

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Aedes nigrinus (Eckstein, 1918) (Diptera, Culicidae), a new country record for England, contrasted with Aedes sticticus (Meigen, 1838)

Cited by 10 publications

References 16 publications

DNA barcoding of British mosquitoes (Diptera, Culicidae) to support species identification, discovery of cryptic genetic diversity and monitoring invasive species

DNA barcoding of British mosquitoes (Diptera, Culicidae) to support species identification, discovery of cryptic genetic diversity and monitoring invasive species

Competence of mosquitoes native to the United Kingdom to support replication and transmission of Rift Valley fever virus

The challenge of invasive mosquito vectors in the U.K. during 2016–2018: a summary of the surveillance and control of Aedes albopictus

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