Adverse events after infusions of cryopreserved hematopoietic stem cells depend on non-mononuclear cells in the infused suspension and patient age

Adverse reactions during stem cell infusion in children treated with autologous and allogeneic stem cell transplantation

“…15 Our study found no relationship between granulocyte content and AR, however, the median granulocyte concentration was low in our study among both groups (median granulocyte 24 × 10 9 /L among NAR vs 25 × 10 9 /L among SAR, P = 0.9). Other reports have similarly identified granulocytes, 15,19 non-mononuclear cells, 13 WBC, 26 and total nucleated cell dose 17 as important risk factors for developing ARs.…”

“…Elevation in serum lactate dehydrogenase levels, a reflection of cell lysis, from day − 1 to +1, was higher in the group of patients who had an AR compared with the group that had none. 13 Post-thaw CD45+ cell viability, a reflection of granulocyte lysis, was lower in a group of patients that had ARs compared with those without. 12 Interestingly, we found no association between WBC/kg, CD45+ cells/kg, mononuclear cells/kg, granulocytes/kg and risk of ARs.…”

“…14 Overall, ARs occurs during infusion of stem cells in 23-76% of cryopreserved products, 1,2,14,19 but almost all studies include only adults or a few pediatric patients but describe the population in aggregate. 13,16,17,19 Our objective was to determine the incidence and grade of ARs occurring during the infusion of CTPs in pediatric patients undergoing allogeneic and autologous HSCT. We also examined risk factors for the development of ARs.…”

“…11 High WBC concentration, especially due to granulocytes or PMNs, has been associated with increased frequency and severity of ARs. [12][13][14][15] Other risk factors include patient age and non-mononuclear cells, 13 total nucleated cell dose, 16,17 RBC content 18 and infusion technique. 14 Overall, ARs occurs during infusion of stem cells in 23-76% of cryopreserved products, 1,2,14,19 but almost all studies include only adults or a few pediatric patients but describe the population in aggregate.…”

Plasma exchange after hematopoietic stem cell transplantation in multiple myeloma to reduce renal insufficiency

“…3 It has been reported that adverse events following infusions of cryopreserved hematopoietic stem cells is associated with the concentration of red blood cells in the infused specimen. 4 Therapeutic plasma exchange (plasmapheresis) removes large molecular weight substances from the plasma, including plasma-free hemoglobin. Plasma-free hemoglobin is the amount of hemoglobin free in plasma solution and is a result of red blood cell breakage or hemolysis.…”