Adventitious root induction in Ophiorrhiza prostrata: a tool for the production of camptothecin (an anticancer drug) and rapid propagation

Abstract

Roots of Ophiorrhiza prostrata D. Don serve as a rich source of camptothecin (CPT), an anticancer drug. Because of the large-scale collection of its roots, the plant has become a threatened species. The present study accomplishes the induction of adventitious roots as a means for the production of CPT as well as for the large-scale propagation of this anticancer drug plant using leaf and internode explants. The biomass yield and CPT content of adventitious roots induced from different explants were compared to roots developed on ex vitro rooted stem cuttings. Adventitious roots were produced on half-strength Murashige and Skoog (MS) medium supplemented with 10.74 lM a-naphthaleneacetic acid and 2.32 lM kinetin at mean fresh weights of 0.753, 0.739 and 0.748 g roots from leaf, internode and shoot, respectively. CPT yield from in vitro derived roots after 50, 80 and 120 days of incubation (0.028, 0.06 and 0.1% dry weight, respectively) was not significantly different from those harvested at the same age from ex vitro rooted (0.03, 0.06 and 0.13%, respectively) stem cuttings. CPT from subcultured roots derived from solid (0.08%) medium was lower than from suspension culture medium (0.12%). Subsequent cultures of the adventitious roots showed a stable production of CPT (0.16%). The yield of CPT from 360-dayold plant-derived roots was 0.19%. Elicitation using methyl jasmonate and acetyl salicylic acid exhibited no enhancement in CPT yield. In vitro propagation through direct shoot regeneration was achieved from the adventitious roots upon transfer to MS medium with 8.87 lM N 6 -benzyladenine (BA) and 2.46 lM indole-3-butyric acid (IBA) with a mean of 21.2 shoots per culture in 50 days. The shoots upon subculture on medium having the same level of BA and IBA underwent rapid proliferation. The shoots transferred to field conditions after in vitro rooting exhibited 95% survival. Adventitious root induction, from leaf and internode explants, enables the feasible production of CPT as well as the large-scale rapid propagation of this species which can safeguard it from extinction.

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

“…In our experiment, 0.4 mg L -1 of IBA was more effective auxins as compared to the other auxin such as IAA and ABA in developing the root systems. This observation has also been reported elsewhere, for example IBA showed a better auxin for adventitious root growth and development in W. somnifera (Baraldi et al 1995), O. prostrata (Martin et al 2008), O. stamineus (Leng and Lai-Keng 2004), P. corylifolia (Baskaran and Jayabalan 2009) and E. angustifolia (Baskaran and Jayabalan 2009).…”

Effects of Media Composition and Auxins on Adventitious Rooting of Bienertia sinuspersici Cuttings

“…It is useful for the growth of most plant species, but its formulation is prepared in very high concentrations, particularly nitrogen, and may be desired in as much as a 10-fold dilution by a particular species and explant type [13]. Thus, lowering the strength of MS medium is often used in vitro culture for rooting of cuttings, as reported in "Delicious" apple, Malus domestica [14] and Ophiorrhiza prostrata, an anti-cancer drug producing plant [15]. This study further confirms that lowering the strength of MS medium was essential for inducing adventitious root formation in B. sinuspersici cuttings and that half-strength MS medium was determined to be the optimal concentration for this process.…”

Phenolic acid and DNA contents of micropropagated Eryngium planum L.

“…Apiaceae roots have the potential to accumulate secondary metabolites, making them a good source for the production of therapeutic compounds. (Ekiert 2000;Martin et al 2008;Murthy et al 2008). Therefore, it is important that successful techniques for the mass culture of Apiaceae Values not significantly different at P = 0.05 (t-Student's test) Fig.…”