Advantages of a new agar medium in the primary isolation of Mycobacterium bovis

Cousins, Debby; Francis, B.R.; Gow, B.L.

doi:10.1016/0378-1135(89)90010-2

Cited by 25 publications

(27 citation statements)

References 5 publications

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“…B83 and ST exhibited similar results in terms of faster detection, which supports Corner's (1994) data from studies using 491 samples, though other investigations showed lower time to first appearance of colonies on 7H11 (COUSINS et al, 1989;CORNER et al, 2012) and Middlebrook 7H11 medium with addition of antibiotics (GALLAGHER;HORWILL, 1977;CORNER;NICOLACOPOULOS, 1988).…”

Section: Discussionsupporting

confidence: 81%

“…Two egg-based media, Stonebrink's medium (ST) (CENTRO PANAMERICANO DE ZOONOSIS, 1985) and Löwenstein-Jensen's medium with sodium pyruvate (LJp) (CORNER; NICOLACOPOULOS, 1988), and two agar-based media, tuberculosis blood agar (B83) (CORNER; NICOLACOPOULOS, 1988) and Middlebrook 7H11 medium (7H11) (COUSINS et al, 1989), were prepared with amounts of 7.5 mL in slopes with screw caps and slopes closed with hydrophobic cotton.…”

Section: Methodsmentioning

confidence: 99%

“…Since M. bovis field strains require enriched media for growth on primary isolation and minimal toxicity of decontaminant reagents (CORNER, 1994), there are several studies seeking the best performance medium (SCHAEFER, 1952;BIRN, 1965;GALLAGHER;HORWILL, 1977;CORNER;NICOLACOPOULOS, 1988;COUSINS et al, 1989), as well as for the best decontamination method (CORNER; TRAJSTMAN, 1988;AMBROSIO et al, 2008;CORNER et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The microaerophilic characteristic has been used as one of the classical bacteriology methods to differ M. bovis from M. tuberculosis (MARKS, 1972;MARKS, 1976;COLLINS et al, 1982). However, the carbon dioxide (CO 2 ) requirement in the incubation atmosphere is questionable when studies without its addition presented growth (COUSINS et al, 1989), while others included CO 2 and had no effect on growth rate or number of colonies (CORNER, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Influence of the incubation conditions on culture media to optimize primary isolation of Mycobacterium bovis

Ikuta

Morato

Souza

et al. 2016

SCA

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The isolation of Mycobacterium bovis is critical to a surveillance system for bovine tuberculosis based on detection of lesions in abattoirs. Thus, four solid culture media and three incubation conditions were investigated to elucidate which combination overcomes the others by assessing growth, time to the first appearance of colonies and their number. Ninety-seven samples of granulomatous lesions were submitted to the decontamination procedure by 1-hexadecylpyridinium chloride at 0.75% w/v, and inoculated on two egg-based media, Stonebrink's (ST) and Löwenstein-Jensen's with sodium pyruvate (LJp), and two agar-based media, tuberculosis blood agar (B83) and Middlebrook 7H11 medium (7H11). Each medium was incubated at 37°C for 90 days in three incubation conditions: in air, in air containing 10% carbon dioxide (CO 2 ), and in air in slopes closed with burned hydrophobic cotton and subsequently plugged with a cork to create a microaerophilic atmosphere. The colonies appeared faster and in higher number when incubated in air containing 10% CO 2 (p < 0.01), independent of media. B83 showed a faster growth and detected more isolates at 30 days of incubation, when compared to ST (0.0178), LJp (p < 0.0001) and 7H11 (p < 0.0001), though there was no difference between B83, ST and LJp at 60 and 90 days of incubation. 7H11 presented the lowest number of isolates (p < 0.0001) and a longer period for the appearance of the first colony (p < 0.001). According to our findings, the concomitant use of ST and B83 media incubated in air containing 10% CO 2 increases the isolation of M. bovis in a shorter period of time, which improves bovine tuberculosis diagnosis. Key words: Primary isolation. Bovine tuberculosis. Mycobacterium bovis. Culture media. Incubation conditions. ResumoO isolamento do Mycobacterium bovis é fundamental para um sistema de vigilância para tuberculose bovina baseado na detecção de lesões em abatedouro. Assim, quatro meios de cultura sólidos e três condições de incubação foram investigados para elucidar qual combinação supera as outras através da avaliação de crescimento, tempo para o aparecimento da primeira colônia e número de colônias. Noventa e sete amostras de lesões granulomatosas foram submetidas ao processo de descontaminação por cloreto de 1-hexadecilpiridínio a 0,75%, e inoculadas em dois meios a base de ovo, Stonebrink (ST) e Löwenstein-Jensen com piruvato de sódio (LJp), e dois meios a base de ágar, ágar sangue tuberculose (B83) e Middlebrook 7H11 (7H11). Cada meio foi incubado a 37°C por 90 dias, em três condições de incubação: em atmosfera normal, em atmosfera com acréscimo de 10% de dióxido de carbono (CO 2 ), e em atmosfera normal em tubos fechados com algodão hidrófobo queimado e subsequentemente fechado com rolha para criar uma atmosfera microaerófila. As colônias apareceram mais rapidamente e em maior número quando incubadas em atmosfera com 10% de CO 2 (p < 0,01), independente dos meios. As micobactérias cresceram em maior abundância e mais rapidamente no meio B83 aos 30 dias de i...

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Section: Discussionsupporting

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of the incubation conditions on culture media to optimize primary isolation of Mycobacterium bovis

Ikuta

Morato

Souza

et al. 2016

SCA

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“…Dados similares aos observados por Gathogo et al (2012), que inspecionaram 929 carcaças em dois abatedouros no Kênia e observaram que as lesões distribuídas nas carcaças estavam localizadas nos gânglios linfáticos brônquicos e mediastinais, parênquima pulmonar, parênquima hepático e linfonodos localizados em outros tecidos. No exame bacteriológico dos fragmentos de lesões provenientes de bovinos abatidos, os resultados foram compatíveis com a literatura, sendo relatado crescimento das primeiras colônias de M. bovis em meio Stonebrink-leslie de 21 dias (Cousins et al 1989), 36 dias (Corner 1994) e 28 dias (Mota et al 2001). Segundo Corner (1994), o diagnóstico bacteriológico é considerado "padrão ouro", apesar de requerer um longo período para o crescimento das primeiras colônias e identificação da espécie.…”

Section: Discussionunclassified

Múltiplas estirpes de isolados de Mycobacteriumbovis identificados por tipagem molecular em bovinos abatidos em matadouros-frigoríficos

et al. 2014

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Pesq. Vet. Bras. 34(2):103-108, fevereiro 2014 103 RESUMO.-O objetivo do presente trabalho foi utilizar mé-todos bacteriológicos e moleculares para a identificação do Mycobacterium bovis em lesões observadas em carcaças de bovinos durante a inspeção post mortem de rotina em matadouros-frigoríficos com serviço de inspeção oficial. Foi acompanhado o abate e a inspeção de 825.394 bovinos, sadios ao exame ante mortem pelo serviço de inspeção oficial em dez matadouros-frigoríficos do estado da Bahia. Carcaça de 180 bovinos apresentaram lesões sugestivas de tuberculose e por outras linfadenites. No isolamento bacteriano, 25 amostras apresentaram crescimento disgônico de colônias de coloração creme-amareladas em meio de cultura Stonebrink-Leslie. Desses isolados, 14 foram identifi- Múltiplas estirpes de isolados de Mycobacterium bovisidentificados por tipagem molecular em bovinos abatidos em matadouros-frigoríficos The aim of this study was to use bacteriological and molecular methods to identify Mycobacterium bovis in lesions observed in cattle carcasses during routine post-mortem inspection in slaughterhouses with official inspection service. It was accompanied the slaughter and inspection of 825,394 cattle, healthy ante mortem examination by the official inspection service in ten slaughterhouses in the state of Bahia. Carcasses of 180 cattle presented lesions suggestive of tuberculosis and other lymphadenitis. In bacterial isolation, 25 samples showed dysgonic growth of colonies of creamy-yellow in medium-Stonebrink Leslie. From these isolates, 14 were identified as M. bovis and the multiplex PCR technique spoligotyping was discriminated against eight different spoligotypes of M. bovis, seven previously described in the literature and a new spoligotypes without former description. The major spoligotypes was SB0121, with five samples which has been described in Brazil and other countries, followed by two clusters, SB295 and SB1055, with two isolates each. The SB1145 and SB1648 spoligotypes were reported only in Brazil and Denmark, respectively. The spoligotypes SB140 has been found in Brazil, Argentina, Uruguay and Paraguay. These results demonstrate that the spoligotypes obtained are shared, so far, among Brazilian states and among Latin America and Europe. Thus, molecular discrimination of isolates of M. bovis by Spoligotyping constitutes a tool for epidemiological studies of bovine tuberculosis in the state of Bahia. INDEX TERMS: Mycobacterium bovis, mycobacteria, molecular epidemiology, spoligotyping, tuberculosis, zoonosis, cattle. (Brasil 2006, Heinemann et al. 2008.A tuberculose no homem é causada principalmente pelo Mycobacterium tuberculosis, mas a doença causada por M. bovis tem sido reportada e representa um risco para a saúde pública (Parreiras et al. 2012, Reyes et al. 2012. Em 2011, 8,7 milhões de pessoas adoeceram e 1,4 milhões de óbitos anuais foi determinado pela tuberculose. A maioria dos óbitos ocorre em países de baixa e média renda e está entre as três principais causas de mortes em mulheres ent...

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Occupational exposure and challenges in tackling M. bovis at human–animal interface: a narrative review

Devi

Lee²,

Yan

et al. 2021

Int Arch Occup Environ Health

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Zoonotic tuberculosis caused by Mycobacterium bovis (M. bovis) , a member of Mycobacterium tuberculosis complex (MTBC) has increasingly gathered attention as a public health risk, particularly in developing countries with higher disease prevalence. M. bovis is capable of infecting multiple hosts encompassing a number of domestic animals, in particular cattle as well as a broad range of wildlife reservoirs. Humans are the incidental hosts of M. bovis whereby its transmission to humans is primarily through the consumption of cattle products such as unpasteurized milk or raw meat products that have been contaminated with M. bovis or the transmission could be due to close contact with infected cattle. Also, the transmission could occur through aerosol inhalation of infective droplets or infected body fluids or tissues in the presence of wound from infected animals. The zoonotic risk of M. bovis in humans exemplified by miscellaneous studies across different countries suggested the risk of occupational exposure towards M. bovis infection, especially those animal handlers that have close and unreserved contact with cattle and wildlife populations These animal handlers comprising of livestock farmers, abattoir workers, veterinarians and their assistants, hunters, wildlife workers as well as other animal handlers are at different risk of contracting M. bovis infection, depending on the nature of their jobs and how close is their interaction with infected animals. It is crucial to identify the underlying transmission risk factors and probable transmission pathways involved in the zoonotic transmission of M. bovis from animals to humans for better designation and development of specific preventive measures and guidelines that could reduce the risk of transmission and to protect these different occupational-related/populations at risk. Effective control and disease management of zoonotic tuberculosis caused by M. bovis in humans are also hindered by various challenges and factors involved at animal–human interface. A closer look into factors affecting proper disease control and management of M. bovis are therefore warranted. Hence, in this narrative review, we have gathered a number of different studies to highlight the risk of occupational exposure to M. bovis infection and addressed the limitations and challenges underlying this context. This review also shed lights on various components and approaches in tackling M. bovis infection at animal–human interface.

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Advantages of a new agar medium in the primary isolation of Mycobacterium bovis

Cited by 25 publications

References 5 publications

Influence of the incubation conditions on culture media to optimize primary isolation of Mycobacterium bovis

Influence of the incubation conditions on culture media to optimize primary isolation of Mycobacterium bovis

Múltiplas estirpes de isolados de Mycobacteriumbovis identificados por tipagem molecular em bovinos abatidos em matadouros-frigoríficos

Occupational exposure and challenges in tackling M. bovis at human–animal interface: a narrative review

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