Advantages and Applications of Cryopreservation in Fisheries Science

Abstract

Cryopreservation is a long-term

Bio-banking: An Emerging Approach for Conservation of Fish Germplasm

“…Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for an extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity [13]. Genetic and taxonomic information serve as a backbone for the implementation of plans to protect and preserve threatened and endangered species.…”

“…Successful cryopreservation of fish sperm have been achieved for more than 200 fish species and many fish species have been adequated for the purpose of cryobanking [18][19][20]. Cryopreservation of sperm have been established for freshwater and marine fish species, including carp, salmonids, rainbow trout, catfish, cichlids, medakas, whitefish, pike, milkfish, grouper, cod, and zebrafish [21][22][23][24][25][26][27].…”

“…A technique to obtain the mature eggs from the late stage oocytes is available. Thus, the combination of this technique and their cryopreservation could be a breakthrough [13]. The studies also indicated that in cryopreserved ovarian tissues, the ovarian follicles remain in their natural three-dimensional structure where they may be protected from physical stress and damage.…”

Cryopreserved sperm for oocyte fertilization of dourado Salminus brasiliensis

“…This technique also facilitates genetic programs implementation, because the easily handling of semen is useful for developing new strains. In addition, one of the most important factors is the possibility of store germplasm, ensuring the maintenance of genetic variability of endangered populations (TSAI & LIN, 2012;MIGAUD et al, 2013).…”

Assessment of viability of sperm cells of Litopenaeus vannamei on cryopreservation

“…The main aim of reproduction biotechnologies is the optimization of male and female gametes and production of individuals with recognized origin, emphasizing genetic inheritance qualities. The techniques of germplasm cryopreservation include storage of the sperm, eggs and embryos and contribute directly to animal breeding programs (Tsai and Lin 2012) such as hybridization, hatcheries, population conservation and genetic selection, making the programs of genetic improvement in the species of economic interest more favorable (Gwo 2000). In this context, semen cryopreservation can be considered an interesting alternative since the resources availability, limitations and chances of failure concerning the material period of storage are observed.…”