Advances in tissue engineering: Cell printing

Varghese, David; Deshpande, Malay; Xu, Tao; Kesari, Priya; Ohri, Sunil K.; Boland, Thomas

doi:10.1016/j.jtcvs.2004.06.050

Cited by 62 publications

(19 citation statements)

References 12 publications

(18 reference statements)

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“…Cell seeding methods using vacuum results in homogenous distribution of the cells; however, all of the published methods require a vacuum pump and the complete seeding process takes almost half an hour [17,25]. Recently, a new method 'cell printing' has been reported [29,30]. Cells and scaffold can be deposited at the same time by this mechanism, but this is still highly experimental.…”

Section: Discussionmentioning

confidence: 99%

A 1-min Method for Homogenous Cell Seeding in Porous Scaffolds

2010

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The aim of this study was to develop and evaluate a simple and rapid cell seeding procedure for both calcium phosphate ceramic scaffolds and polymer scaffolds. Poly(d,l-lactic acid) and β-tri-calcium phosphate scaffolds were seeded with MC3T3-E1 cells in a syringe. Scaffolds were put in the syringe. After replacing the plunger, the cell suspension was drawn into the syringe. The syringe was closed and the plunger was retracted to the volume of the cell suspension to create a vacuum. This was done for 3 × 10 s. By this procedure, cells were homogenously distributed throughout the scaffold. The efficiency of cell seeding was approximately 60% for both scaffolds independent of the initial cell density. The hypotension the cells experienced for 3 × 10 s did not affect the proliferation capacity of the cells. In conclusion, this method of syringe-vacuum cell seeding is easy, quick, cheap, and easily to perform at an operating theatre.

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Section: Discussionmentioning

confidence: 99%

A 1-min Method for Homogenous Cell Seeding in Porous Scaffolds

2010

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“…Furthermore, the maintenance of neuronal phenotypes and basic electrical functions of the cortical neurons in the printed patterns was verified. This method, when combined with the 3D tissue printing method we have demonstrated previously [8], offers the capability of the modified inkjet printers to fabricate 3D functional neural and cardiac tissues, which may have potential in the regenerative treatments for serious neural and cardiac injuries and diseases.…”

Section: Discussionmentioning

confidence: 99%

Fabricating Neural and Cardiomyogenic Stem Cell Structures by a Novel Rapid Prototyping—the Inkjet Printing Method

Gregory

Molnár

et al. 2004

MRS Proc.

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Direct printing of living cardiomyogenic stem cells and embryonic cortical neurons to generate complex cellular patterns and structures of such cells was demonstrated in the study. Furthermore, the immunostaining analyses and the whole-cell patch clamp recordings showed the cortical neurons grown in the printed cellular patterns and structures maintained their basic cellular functions, including neuronal phenotypes and electrophysiological properties. These results and findings may greatly prompt the inkjet printing method evolving into a viable and cost-effective approach for engineering human neural and cardiac tissues or even organs.

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“…Another method of delivery that is currently in development is bioprinting. This method has the capability to deliver specific cells and biomaterials to predefined target sites using layer-by-layer freeform fabrication [23,[42][43][44][45][46]. Typical freeform fabrication involves printing cells onto a scaffold.…”

Section: Experimental Studiesmentioning

confidence: 99%