Advances in the Conservation of Bamboo Genetic Resources Through Whole Seed Cryopreservation

Scherwinski‐Pereira, Jonny Everson; Nogueira, Jênifer Silva; Vasconcelos, Janaína Medeiros; Neves, Joane dos Santos; Balzon, Talita Aparecida

doi:10.1007/978-981-16-1310-4_12

Cited by 1 publication

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“…The association of this technique with cryopreservation represents a perfect match for bamboo propagation and conservation; however, no reports exploiting cryopreservation and somatic embryogenesis have been described [ 11 ]. Only two reports on the slow-growth conservation of somatic embryos of Dendrocalamus hamiltonii [ 12 , 13 ] and one on cryopreservation of the whole zygotic seed [ 14 ] were available to date, demonstrating the need for new strategies for the in vitro conservation of bamboo species.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Antioxidant System Activation Assures the Viability of Guadua chacoensis (Bambusoideae, Poaceae) Embryogenic Cultures during Cryopreservation

Polesi

Goeten

Fraga

et al. 2023

Plants

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This study aimed to establish a cryopreservation protocol for G. chacoensis embryogenic cultures (ECs) and to investigate the role of antioxidant enzymes activities during cryopreservation. The growth dynamics of cell suspensions were also investigated, followed by a phytotoxicity test to assess the ECs’ ability to tolerate the use of cryoprotective solutions for different incubation times (0, 30, 60, 120, and 240 min). We evaluated the EC redox state in three steps of cryopreservation: after incubation in cryoprotection solution, after thawing, and 60 days after regrowth. Our results showed that the ECs support the use of cryoprotective solution until 120 min, showing phytotoxic effects with 240 min of incubation. This study reports a 100% survival of the cultures and a 10% increase ratio in fresh material for both incubation times tested (60 and 120 min). Increased malonaldehyde content was identified after incubation in the cryoprotective solution. An increase in the activities of catalase and ascorbate peroxidase was also identified in the subsequent steps, suggesting that the activation of antioxidant enzymes is essential for maintaining cell homeostasis during cryopreservation.

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Section: Introductionmentioning

confidence: 99%