Advances in sequencing and key character analysis of mango (Mangifera indica L.)

Abstract

Mango (Mangifera indica L.) is an important fruit crop in tropical and subtropical countries associated with many agronomic and horticultural problems, such as susceptibility to pathogens, including powdery mildew and anthracnose, poor yield and quality, and short shelf life. Conventional breeding techniques exhibit significant limitations in improving mango quality due to the characteristics of long ripening, self-incompatibility, and high genetic heterozygosity. In recent years, much emphasis has been placed on identification of key genes controlling a certain trait through genomic association analysis and directly breeding new varieties through transgene or genotype selection of offspring. This paper reviews the latest research progress on the genome and transcriptome sequencing of mango fruit. The rapid development of genome sequencing and bioinformatics provides effective strategies for identifying, labeling, cloning, and manipulating many genes related to economically important traits. Preliminary verification of the functions of mango genes has been conducted, including genes related to flowering regulation, fruit development, and polyphenol biosynthesis. Importantly, modern biotechnology can refine existing mango varieties to meet the market demand with high economic benefits.

Increasing Mango Production Efficiency under the Fast-Changing Climate

“…Recent genome sequences on mango varieties (Carabao, Tommy Atkins, Alphonso & Irwin) facilitate the study of mangoes at the transcriptomics, comparative genomics and genotyping to provide reliable gene sets and references [75][76][77]. A number of molecular markers are also used to explore genetic diversity, generate genetic linkage maps and identify markers for key traits (pulp colour, fruit colour, taste and flavour) [54,[78][79][80]. These investigations aid in the identification of the genes and proteins involved in early and late flowering, various stages of fruit development and biotic stress situations.…”

SSR Markers-Based DNA Fingerprinting for Varietal Identification in Mango Cultivars

“…Mango is often cross-pollinated [4], an allotetraploid (2n = 4X= 40) [5], highly heterozygous tree fruit, mono-and polyembryonic seed and having small imputed genome size of approximately 440-480 MB [6,7]. The genes controlling the traits can be mapped using high-throughput sequencing [8,9]. New mango varieties are generated by hybridization, introduction, selection and breeding of novel transgenes or genotypes [10,11].…”