Advances in research on signal molecules regulating biofilms

Li, Yang; Li, Jinpeng; Liu, Baobao; Wang, Yang

doi:10.1007/s11274-019-2706-x

Cited by 27 publications

(21 citation statements)

References 51 publications

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“…Most AHL synthases have the exact AHL synthesis mechanism; however, their substrate interaction mechanism and identification differ depending on the quantity of acyl-ACP substrates. The availability of LuxI crystallographic structures has paved the way for a better understanding of substrate–enzyme interactions at the molecular level [ 14 , 50 ]. However, the crystal structure of the AHL synthase coupled to the acyl-ACP complex has not yet been determined, which limits our ability to comprehend the interaction details.…”

Section: Ahl Signalmentioning

confidence: 99%

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

et al. 2022

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Microbial biodiversity includes biotic and abiotic components that support all life forms by adapting to environmental conditions. Climate change, pollution, human activity, and natural calamities affect microbial biodiversity. Microbes have diverse growth conditions, physiology, and metabolism. Bacteria use signaling systems such as quorum sensing (QS) to regulate cellular interactions via small chemical signaling molecules which also help with adaptation under undesirable survival conditions. Proteobacteria use acyl-homoserine lactone (AHL) molecules as autoinducers to sense population density and modulate gene expression. The LuxI-type enzymes synthesize AHL molecules, while the LuxR-type proteins (AHL transcriptional regulators) bind to AHLs to regulate QS-dependent gene expression. Diverse AHLs have been identified, and the diversity extends to AHL synthases and AHL receptors. This review comprehensively explains the molecular diversity of AHL signaling components of Pseudomonas aeruginosa, Chromobacterium violaceum, Agrobacterium tumefaciens, and Escherichia coli. The regulatory mechanism of AHL signaling is also highlighted in this review, which adds to the current understanding of AHL signaling in Gram-negative bacteria. We summarize molecular diversity among well-studied QS systems and recent advances in the role of QS proteins in bacterial cellular signaling pathways. This review describes AHL-dependent QS details in bacteria that can be employed to understand their features, improve environmental adaptation, and develop broad biomolecule-based biotechnological applications.

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Section: Ahl Signalmentioning

confidence: 99%

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

et al. 2022

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“…The primary mechanism by which c-di-GMP regulates cell cycle progression, bacterial motility, and biofilm formation involves the interaction of c-di-GMP with a wide assortment of effectors, including riboswitches, DNA-binding proteins, protein complexes, and enzymes whose activities are allosterically regulated through binding of c-di-GMP (Valentini and Filloux, 2019). Several recent reviews thoroughly describe how c-di-GMP binding to these effectors influences transcriptional regulation, signaling, and bacterial virulence (Valentini and Filloux, 2019;Yi et al, 2019;Kunz and Graumann, 2020;Ma et al, 2020;Yoon and Waters, 2021). This review will specifically focus on how c-di-GMP post-translationally regulates biofilm formation through allosteric activation of enzyme complexes involved in the biosynthesis and export of biofilm exopolysaccharides that serve as key structural components of the biofilm EPS.…”

Section: Introductionmentioning

confidence: 99%

Regulation of Biofilm Exopolysaccharide Production by Cyclic Di-Guanosine Monophosphate

Poulin

Kuperman

2021

Front. Microbiol.

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Many bacterial species in nature possess the ability to transition into a sessile lifestyle and aggregate into cohesive colonies, known as biofilms. Within a biofilm, bacterial cells are encapsulated within an extracellular polymeric substance (EPS) comprised of polysaccharides, proteins, nucleic acids, lipids, and other small molecules. The transition from planktonic growth to the biofilm lifecycle provides numerous benefits to bacteria, such as facilitating adherence to abiotic surfaces, evasion of a host immune system, and resistance to common antibiotics. As a result, biofilm-forming bacteria contribute to 65% of infections in humans, and substantially increase the energy and time required for treatment and recovery. Several biofilm specific exopolysaccharides, including cellulose, alginate, Pel polysaccharide, and poly-N-acetylglucosamine (PNAG), have been shown to play an important role in bacterial biofilm formation and their production is strongly correlated with pathogenicity and virulence. In many bacteria the biosynthetic machineries required for assembly of these exopolysaccharides are regulated by common signaling molecules, with the second messenger cyclic di-guanosine monophosphate (c-di-GMP) playing an especially important role in the post-translational activation of exopolysaccharide biosynthesis. Research on treatments of antibiotic-resistant and biofilm-forming bacteria through direct targeting of c-di-GMP signaling has shown promise, including peptide-based treatments that sequester intracellular c-di-GMP. In this review, we will examine the direct role c-di-GMP plays in the biosynthesis and export of biofilm exopolysaccharides with a focus on the mechanism of post-translational activation of these pathways, as well as describe novel approaches to inhibit biofilm formation through direct targeting of c-di-GMP.

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“…Wszystkie razem te komponenty budują skomplikowaną i rozległą sieć regulatorową [101]. Jednym z istotniejszych TCS u tego patogenu jest system GacS/A [115]. Kinaza histydynowa GacS przenosi grupę fosforanową na regulator odpowiedzi GacA, który ufosforylowany reguluje ekspresję m.in.…”

Section: Wpływ Dwuskładnikowych Systemów Transdukcji Sygnału I Srna N...unclassified

“…Te zaś mogą zapobiegać wiązaniu się rybosomu z sekwencją SD i hamować translację mRNA rsmA, kodującego regulator transkrypcji, który jest homologiem CsrA [51]. RsmA kontroluje ekspresję genów systemu sekrecji III typu (T3SS), wytwarzania pili IV typu, ruchliwości oraz wytwarzania biofilmu [115]. RetS i LadS to hybrydowe kinazy sensorowe, które uczestniczą w kontroli ekspresji systemu T3SS oraz tworzenia biofilmu [8].…”

Section: Wpływ Dwuskładnikowych Systemów Transdukcji Sygnału I Srna N...unclassified

Oddziaływania Pomiędzy Małymi, Regulatorowymi RNA a Dwuskładnikowymi Systemami Transdukcji Sygnału u Bakterii Gram-Ujemnych

Jaworska

Staniszewska

Gomza

et al. 2022

Postępy Mikrobiologii - Advancements of Microbiology

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Streszczenie Małe, regulatorowe RNA (sRNA) wraz z dwuskładnikowymi systemami transdukcji sygnału (TCS) wchodzą w skład rozbudowanych sieci kontroli ekspresji genów bakteryjnych. Oddziałując ze sobą wzajemnie, zapewniają niezwykle szybką, jak również precyzyjną odpowiedź bakterii na zmieniające się warunki bytowania. Kontrolując wiele procesów wpływają na stan fizjologiczny komórki, skład proteomu osłon komórkowych czy zdolność do wzrostu w postaci biofilmu. Niezwykle często sRNA stanowią brakujące ogniwa, w odpowiedzi na złożone bodźce środowiskowe, pomiędzy dwuskładnikowymi systemami transdukcji sygnału, a genami docelowymi czy innymi systemami regulatorowymi. Współdziałanie TCS-sRNA wydaje się globalną cechą regulacyjną u wielu organizmów prokariotycznych. Zdobywanie wiedzy na temat tych mechanizmów kontroli toruje drogę do opracowania nowych strategii walki z drobnoustrojami patogennymi.

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Advances in research on signal molecules regulating biofilms

Cited by 27 publications

References 51 publications

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

Regulation of Biofilm Exopolysaccharide Production by Cyclic Di-Guanosine Monophosphate

Oddziaływania Pomiędzy Małymi, Regulatorowymi RNA a Dwuskładnikowymi Systemami Transdukcji Sygnału u Bakterii Gram-Ujemnych

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