Advances in multiparametric molecular diagnostics technologies for respiratory tract infections

Reddington, Kate; Tuite, Nina; Barry, Thomas; O’Grady, Justin; Zumla, Alimuddin

doi:10.1097/mcp.0b013e32835f1b32

Cited by 26 publications

(24 citation statements)

References 26 publications

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“…However, the development of such tests and their successful implementation into clinical practice requires further development. [31][32][33] Where the accuracy of results is high with multiplex tests, the desirable characteristics for providing both diagnostic and epidemiological information become convergent, and routine diagnostic laboratories can consider fulfi lling a public health role. 34 Molecular multiplex tests need to be transported outside the laboratory as point-of-care tests in busy tertiary care, outpatient clinical settings, or rural areas in developing countries.…”

Section: Point-of-care and Near-patient Testingmentioning

confidence: 99%

Rapid point of care diagnostic tests for viral and bacterial respiratory tract infections—needs, advances, and future prospects

Zumla

Al‐Tawfiq

Enne

et al. 2014

The Lancet Infectious Diseases

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147

132

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Respiratory tract infections rank second as causes of adult and paediatric morbidity and mortality worldwide. Respiratory tract infections are caused by many different bacteria (including mycobacteria) and viruses, and rapid detection of pathogens in individual cases is crucial in achieving the best clinical management, public health surveillance, and control outcomes. Further challenges in improving management outcomes for respiratory tract infections exist: rapid identification of drug resistant pathogens; more widespread surveillance of infections, locally and internationally; and global responses to infections with pandemic potential. Developments in genome amplification have led to the discovery of several new respiratory pathogens, and sensitive PCR methods for the diagnostic work-up of these are available. Advances in technology have allowed for development of single and multiplexed PCR techniques that provide rapid detection of respiratory viruses in clinical specimens. Microarray-based multiplexing and nucleic-acid-based deep-sequencing methods allow simultaneous detection of pathogen nucleic acid and multiple antibiotic resistance, providing further hope in revolutionising rapid point of care respiratory tract infection diagnostics.

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Section: Point-of-care and Near-patient Testingmentioning

confidence: 99%

Rapid point of care diagnostic tests for viral and bacterial respiratory tract infections—needs, advances, and future prospects

Zumla

Al‐Tawfiq

Enne

et al. 2014

The Lancet Infectious Diseases

Self Cite

147

132

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“…Furthermore, the filmarray GI panel detects and identifies the greatest number of viruses, bacteria and parasites on the market at present. However, a disadvantage of the Filmarray platform is that sample throughput is low [36].…”

Section: Integrated Platform Testsmentioning

confidence: 99%

A current overview of commercially available nucleic acid diagnostics approaches to detect and identify human gastroenteritis pathogens

Reddington

Tuite

Minogue

et al. 2014

Biomolecular Detection and Quantification

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Purpose of reviewGastroenteritis is caused by a wide range of viral, bacterial and parasitic pathogens and causes millions of deaths worldwide each year, particularly in infant populations in developing countries. Traditional microbiological culture and immunological based tests are time consuming, laborious and often lack diagnostic specificity and sensitivity. As a result patients can receive suboptimal and/or inappropriate antimicrobial treatment. In recent years, rapid nucleic acid diagnostics (NAD) technologies have become available to complement or even bypass and replace these traditional microbiological culture and immunological based tests.The main purpose of this review is to describe a number of recently available multiparametric commercial tests, to support the rapid and accurate clinical diagnosis of human gastroenteritis. These state of the art technologies have the ability to identify a wide range of microorganisms associated with enteric gastroenteritis. Following further technological innovation and more comprehensive clinical validation studies, these NAD tests have the potential to impact on the economic burden of health care systems. These rapid NAD tests can also be used to guide improved patient therapy in a timely manner which will reduce the extent of morbidity and mortality associated with these infections globally.

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“…However, the low multiplexing capability of real-time PCR instrumentation represents a major drawback of the technology within the clinical setting [28], which is limited to four or five fluorescent channels detecting fluorescent dyes at different wavelengths or using melting-point analysis to differentiate among PCR products. Limitations are associated with the number of detectors available or the need for discrimination in detectable wavelengths to prevent cross-talk.…”

Section: Introductionmentioning

confidence: 99%

The increasing application of multiplex nucleic acid detection tests to the diagnosis of syndromic infections

Gray

Coupland

2013

Epidemiol. Infect.

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On 14 January 2013, the US Food and Drug Administration (FDA) announced permission for a multiplex nucleic acid test, the xTAG® Gastrointestinal Pathogen Panel (GPP) (Luminex Corporation, USA), which simultaneously detects 11 common viral, bacterial and parasitic causes of infectious gastroenteritis, to be marketed in the USA. This announcement reflects the current move towards the development and commercialization of detection technologies based on nucleic acid amplification techniques for diagnosis of syndromic infections. We discuss the limitations and advantages of nucleic acid amplification techniques and the recent advances in Conformité Européene - in-vitro diagnostic (CE-IVD)-approved multiplex real-time PCR kits for the simultaneous detection of multiple targets within the clinical diagnostics market.

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Advances in multiparametric molecular diagnostics technologies for respiratory tract infections

Cited by 26 publications

References 26 publications

Rapid point of care diagnostic tests for viral and bacterial respiratory tract infections—needs, advances, and future prospects

Rapid point of care diagnostic tests for viral and bacterial respiratory tract infections—needs, advances, and future prospects

A current overview of commercially available nucleic acid diagnostics approaches to detect and identify human gastroenteritis pathogens

The increasing application of multiplex nucleic acid detection tests to the diagnosis of syndromic infections

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