Advances in measuring lifespan in the yeast
            <i>Saccharomyces cerevisiae</i>

Minois, Nadège; Frajnt, Magdalena; Wilson, Chris; Vaupel, James W.

doi:10.1073/pnas.0408332102

Cited by 76 publications

(57 citation statements)

References 25 publications

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“…Since overexpression leads to about a 20% increase in longevity in yeast, it may be that the effect is smaller or more variable in worms and therefore undetectable. Moreover, the effect in yeast is on replicative and not chronological life span, although replicative life span correlates well with total yeast life span (Minois et al 2005). When hyl-1 deletion mutants became available in worms, we assessed their longevity, since the longevity effect in yeast LAG1 deletions is on the order of 50%.…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1

Tedesco

Jiang

Wang

et al. 2008

AGE

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Yeast LAG1 was one of the first longevity genes found. Subsequent analysis showed that it encodes a component of ceramide synthase. Homologs of LAG1 have been identified in all eukaryotes examined for their presence, and multiple homologs are the norm. In human and mouse, the LAG1 counterpart is called LASS1. The involvement of this gene in determining yeast replicative life span led us to ask whether longevity effects could be found in C. elegans. Extended longevity was seen when we used RNAi to decrease expression of the worm homolog of LAG1, termed hyl-1, for Homolog of Yeast Longevity gene. In contrast, neither deletion of the gene nor overexpression resulted in life extension. There was no evidence that hyl-1 interacts with the insulin/IGF-1 like signaling pathway to specify longevity or dauer formation, nor were effects on stress resistance detected. Gene expression of hyl-1 homologs was altered in the deletion mutant and by RNAi, showing distinct evidence for compensation at the transcript level. These regulatory changes may explain the subtle phenotypic effects found under the conditions studied here.

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Section: Discussionmentioning

confidence: 99%

Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1

Tedesco

Jiang

Wang

et al. 2008

AGE

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“…Recently, we have developed a measurement of life span in individual S. cerevisiae yeast cells, not linked to the ability to divide (Minois et al 2005). We simply used a dye, Phloxine B, able to differentially stain dead and living cells to know exactly when a cell dies, i.e., becomes metabolically inactive.…”

Section: Resultsmentioning

confidence: 99%

Symmetrically Dividing Cells of the Fission Yeast Schizosaccharomyces Pombe Do Age

et al. 2006

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Theories of the evolution of senescence state that symmetrically dividing organisms do not senesce. However, this view is challenged by experimental evidence. We measured by immunofluorescence the occurrence and intensity of protein carbonylation in single and symmetrically dividing cells of Schizosaccharomyces pombe. Cells of S. pombe show different levels of carbonylated proteins. Most cells have little damage, a few show a lot, an observation consistent with the gradual accumulation of carbonylation over time. At reproduction, oxidized proteins are shared between the two resulting cells. These results indicate that S. pombe does age, but does so in a different way from other studied species. Damaged cells give rise to damaged cells. The fact that cells with no or few carbonylated proteins constitute the main part of the population can explain why, although age is not reset to zero in one of the cells during division, the pool of young cells remains large enough to prevent the rapid extinction of the population.

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“…This may have several reasons, but the most probable may be the proliferation of the microorganisms due to the relatively high temperatures of PBS during the 30 min sampling (33 • C without and 25 • C with tempering). S. cerevisiae yeast cells have a generation time of approximately 90 min at 30 • C (Minois et al 2004), which is three times the sampling time in our experiments. However, in laboratory tests, yeast cells were cultivated for 30 min at 33 • C and 25 • C in an Erlenmeyer flask in PBS to evaluate the influence of proliferation within sampling to the number of CFU.…”

Section: Discussionmentioning

confidence: 99%

A Temperature-Controlled AGI-30 Impinger for Sampling of Bioaerosols

Springorum¹,

Clauß²,

Hartung³

2011

Aerosol Science and Technology

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AGI-30 impingers are widely applied for the efficient collection of bioaerosols. However, these glass vessels are not protected against solar radiation and changing ambient temperature during sampling, which may impair the sampling efficiency by too high or low temperatures of the sampling liquid leading to early proliferation of microorganisms or ice formation. In order to reduce such disadvantages, an impinger holder was developed and tested under laboratory and field conditions, which insulates and controls the temperature of the impinger and the sampling liquid by means of a Peltier element. The influence of various ambient temperatures and relative humidities on the temperatures of tempered and untempered impinger liquids such as mineral oil, peptone water, and phosphate-buffered saline (PBS) during sampling periods of 30 min was investigated and compared. The type of sampling liquid greatly influenced the amount of residual liquid after sampling and the temperature in both the tempered and untempered impingers depended on the type of sample liquid, ambient temperature, and humidity. Mineral oil showed the lowest losses, followed by PBS and peptone water. Tempering of the impingers had some influence on the amount of losses. Tempering also strongly influenced the number of culturable bacteria (colony-forming unit [CFU]) in the sampling liquids. In the tempered impingers, three times higher CFU/m 3 were found than in the untempered impingers. A great advantage of tempering was that sampling at ambient temperatures as low as −5 • C was possible. However, in spite of insulation and tempering the impinger to a fixed value, the variance was rather high due to varying ambient temperature and relative humidity, which always have a strong influence on condensation and evaporation and therefore on the amount of sample liquid and the resulting number of CFU. Nevertheless, the temperature-controlled AGI-30 impinger is a step forward to more stable results.

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Advances in measuring lifespan in the yeast Saccharomyces cerevisiae

Cited by 76 publications

References 25 publications

Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1

Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1

Symmetrically Dividing Cells of the Fission Yeast Schizosaccharomyces Pombe Do Age

A Temperature-Controlled AGI-30 Impinger for Sampling of Bioaerosols

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