Advances in electrospinning and 3D bioprinting strategies to enhance functional regeneration of skeletal muscle tissue

Embedded 3D bioprinting – An emerging strategy to fabricate biomimetic & large vascularized tissue constructs

“…Bioinks used for extrusion bioprinting possess high viscosity & shear-thinning behavior to maintain high cell viability, shape fidelity, and layer stackability. Extrusion–based bioprinting is a widely explored and cost–effective method among the earlier mentioned bioprinting techniques [ 21 ]. However, the fabrication of micro-vascularized and anatomically similar complex structures is challenging due to poor resolution [ 22 ].…”

Emerging perspectives on 3D printed bioreactors for clinical translation of engineered and bioprinted tissue constructs

“…1 These engineered tissue scaffolds assist in regenerating dead/damaged tissues that have lost their regeneration potential. 2 In recent times, additive manufacturing has emerged as an alternative to conventional scaffold fabrication techniques owing to the creation of 3D scaffolds with improved properties for cell adhesion, proliferation, extracellular deposition, and tissue regeneration. 3 In particular, 3D bioprinting is capable of fabricating complex living tissue architectures by printing cell-suspended bioinks in a layer-by-layer fashion with high reproducibility.…”

“…The bioreactor cultured scaffold subjected to fluid flow induced mechanical stimulation has improved the cell proliferation and maturation and increased the expression of muscle markers compared to the static cultured 3D scaffold. 2,17,18 Hence, the bioreactor culture approach might address the existing challenges in static in vitro culture conditions due to its ability to provide the native tissue microenvironment. However, advanced research in bioreactor development ended up with additional feasibility of culturing, storing, monitoring and assessing the functionality of the TE constructs for a longer culture period of up to a few months before implantation in vivo.…”

Global hotspots and emerging trends in 3D bioprinting research

“…Due to the superior performance of the 3D bioprinting technology, it has been a research hotspot in various fields of tissue engineering, such as transplant and regenerative medicine, as well as disease model construction. For instance, several types of bio-ink and printing processes have been developed for applications in tissue engineering ( Liang et al 2022 ; Luo et al 2022 ; Murab et al 2022 ; Sonaye et al 2022 ; Thangadurai et al 2022 ). In addition, 3D bioprinting has been widely used in artificial organ fabrication and tissue regeneration ( Ramadan and Zourob, 2020 ; Jain et al 2022 ; Panda et al 2022 ; Pourmasoumi et al 2022 ; Wang et al 2023 ).…”