Advances and applications of nanophotonic biosensors

Altug, Hatice; Oh, Sang‐Hyun; Maier, Stefan A.; Homola, Jiřı́

doi:10.1038/s41565-021-01045-5

Cited by 440 publications

(286 citation statements)

References 155 publications

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“…The characteristics of optical resonances are well studied for individual dielectric particles, while their arrays are used for practical applications. An experimental study of metal particle arrays revealed the effects of collective lattice resonances which provide laser generation 17 , 18 , biosensing applications 19 , 20 and amplification of light radiation 21 – 23 . In addition to creating antireflection coatings, arrays of ordered dielectric particles can be used in optical waveguides 24 , 25 and also as metasurfaces and metamaterials 23 , 26 .…”

Section: Introductionmentioning

confidence: 99%

Interdisk spacing effect on resonant properties of Ge disk lattices on Si substrates

Shklyaev

Utkin

Tsarev

et al. 2022

Sci Rep

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The light reflection properties of Ge disk lattices on Si substrates are studied as a function of the disk height and the gap width between disks. The interdisk spacing effect is observed even at such large gap widths as 500 nm. The gap width decrease leads to the appearance of the reflection minimum in the short wavelength region relative to one originated from the magnetic and electric dipole resonances in individual Ge disks, thereby essentially widening the antireflection properties. This minimum becomes significantly deeper at small gap widths. The observed behavior is associated with the features of the resonant fields around closely spaced disks according to numerical simulation data. The result shows the importance of using structures with geometrical parameters providing the short-wavelength minimum. This can essentially enhance their other resonant properties, which are widely used for applications, in particular, based on collective lattice resonances.

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Section: Introductionmentioning

confidence: 99%

Interdisk spacing effect on resonant properties of Ge disk lattices on Si substrates

Shklyaev

Utkin

Tsarev

et al. 2022

Sci Rep

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“…Because prion proteins have strong interactions with simply functionalized metallic surfaces, besides AuNPs, we envision a broad range of metallic nanoparticles with various materials and shapes to be useful in detection. Additionally, substrate-based nanostructures exhibiting optical resonances could be useful in detecting conformational changes via other sensing modalities such as surface-enhanced infrared absorption spectroscopy 60,61 to further improve the speed and accuracy of prion detection. Examining other areas of the electromagnetic spectrum, such as tera-Hertz sensing, could also lead to improved detection.…”

Section: Discussionmentioning

confidence: 99%

A Field-Deployable Diagnostic Assay for the Visual Detection of Misfolded Prions

Christenson

Rowden

et al. 2021

Preprint

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Diagnostic tools for the detection of protein-misfolding diseases (i.e., proteopathies) are limited. Gold nanoparticles (AuNPs) facilitate sensitive diagnostic techniques via visual color change for the detection of a variety of targets. In parallel, recently developed quaking-induced conversion (QuIC) assays leverage protein-amplification and fluorescent signaling for the accurate detection of misfolded proteins. Here, we combine AuNP and QuIC technologies for the visual detection of amplified misfolded prion proteins from tissues of wild white-tailed deer infected with chronic wasting disease (CWD), a prion disease of cervids. Our newly developed assay, MN-QuIC™, enables both naked-eye and light-absorbance measurements for detection of misfolded prions. MN-QuIC™ leverages basic laboratory equipment that is cost-effective and portable, thus facilitating real-time prion diagnostics across a variety of settings. To test the portability of our assay, we deployed to a rural field-station in southeastern Minnesota and tested for CWD on site. We successfully demonstrated that MN-QuIC™ is functional in a non-traditional laboratory setting by performing a blinded analysis in the field and correctly identifying all CWD positive and CWD not detected deer at the field site in less than 24 hours, thus documenting the portability of the assay. Additionally, we show that electrostatic forces help govern the AuNP/prion interactions. Importantly, all of our white-tailed deer (n=37) were independently tested using ELISA, IHC, and/or RT-QuIC technologies and results secured with MN-QuIC™ were 100% consistent with these tests. We conclude that hybrid AuNP and QuIC assays, such as MN-QuIC™, have great potential for sensitive, field-deployable diagnostics of a variety of protein misfolding diseases.Statement of SignificancePortable diagnostic tools for the detection of prion and protein-misfolding diseases are limited. Using chronic wasting disease (CWD) as a model, we demonstrate the functionality of a gold nanoparticle (AuNP)-based assay for the field-based detection of prion diseases. CWD is spreading through cervid populations across North America and the disease poses significant threats to a number of economic sectors, with global implications. Our newly developed diagnostic assay leverages the visible color change of AuNPs to detect the presence of CWD-causing prions. The assay can be performed using lowcost equipment and reagents, thus having great potential to expand prion disease surveillance efforts and prevent infectious prions from entering the human food chain.

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“…The same biointerface was then used for the analysis of antiHBs in saliva samples, where, due to the lower concentration compared to blood plasma, the analysis had to be performed with the sandwich assay format and PEF readout [27]. Let us note that a thorough review of these developments has been conducted by more specialized publications [28][29][30]. [22] with permission from Elsevier.…”

Section: Chemosensor Types 21 State-of-the-art Of Plasmonic Sensorsmentioning

confidence: 99%

State of the Art of Chemosensors in a Biomedical Context

et al. 2022

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Healthcare is undergoing large transformations, and it is imperative to leverage new technologies to support the advent of personalized medicine and disease prevention. It is now well accepted that the levels of certain biological molecules found in blood and other bodily fluids, as well as in exhaled breath, are an indication of the onset of many human diseases and reflect the health status of the person. Blood, urine, sweat, or saliva biomarkers can therefore serve in early diagnosis of diseases such as cancer, but also in monitoring disease progression, detecting metabolic disfunctions, and predicting response to a given therapy. For most point-of-care sensors, the requirement that patients themselves can use and apply them is crucial not only regarding the diagnostic part, but also at the sample collection level. This has stimulated the development of such diagnostic approaches for the non-invasive analysis of disease-relevant analytes. Considering these timely efforts, this review article focuses on novel, sensitive, and selective sensing systems for the detection of different endogenous target biomarkers in bodily fluids as well as in exhaled breath, which are associated with human diseases.

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Advances and applications of nanophotonic biosensors

Cited by 440 publications

References 155 publications

Interdisk spacing effect on resonant properties of Ge disk lattices on Si substrates

Interdisk spacing effect on resonant properties of Ge disk lattices on Si substrates

A Field-Deployable Diagnostic Assay for the Visual Detection of Misfolded Prions

State of the Art of Chemosensors in a Biomedical Context

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