Advanced fibroblast proliferation inhibition for biocompatible coating by electrostatic layer-by-layer assemblies of heparin and chitosan derivatives

Polyelectrolyte Multilayer Films Based on Natural Polymers: From Fundamentals to Bio-Applications

“…The bioactivity of CHI/HEP films was different from CHI/Dex films and the surface of the CHI/HEP assembly showed strong anticoagulant activity which is useful for coating various blood-contacting biomedical materials [ 24 ]. Chitosan and N-methylated chitosan derivatives, namely, N,N-dimethyl chitosan (DMC) and N,N,N-trimethyl chitosan (TMC) assembled with HEP, showed good inhibition of natural inflammatory response hindering the proliferation speed of fibroblasts up to a factor of forty-fold [ 84 ]. In that sense, multilayer CHI/HEP films have been employed to coat different materials, such as stainless-steel coronary stents and NiTi endovascular stents, to accelerate the re-endothelialization and healing process after coronary stent deployment.…”

“… ( a ) NIH/3T3 fibroblasts after 1 day of contact with controls or LbL multilayer films (left), and surface cell density after 1 and 7 days (right) (Adapted and reprinted with permission from [ 84 ]; Copyright 2016 Elsevier Inc. All rights reserved); ( b ) Adapted and reprinted with permission from [ 90 ] Copyright 2010 Wiley Periodicals, Inc.; ( c ) Schematic representation of the electrostatic interaction between chitosan and hyaluronic acid (left). Adhesive properties of freestanding membranes CHI/HA and CHI/HA-DN (center).…”

Biomimetic Principles to Develop Blood Compatible Surfaces

“…Furthermore, heparin-terminated multilayers were shown to prevent adsorption of major blood proteins such as serum albumin, immunoglobulin G, and fibrinogen onto poly-L-lysine/heparin multilayers, confirming the blood compatibility of this coating concept (121). On the cellular side, a multilayer composed of heparin and chitosan efficiently reduced macrophage adhesion, macrophage spreading, β1 integrin expression, as well as IL-1β production (122), and showed basically no induction of a proliferative response of NIH/3T3 fibroblasts in standard cell culture assays (123). Furthermore, layer-by-layer films of heparin and collagen type I on poly-L-lactic acid substrates were shown to have no activation potential on human mesenchymal stem cells under culture conditions (124), providing additional evidence for the potential of biomimetic coatings using carbohydrate structures to increase blood compatibility (124).…”

N,N,N-Trimethyl Chitosan and Its Potential Bactericidal Activity: Current Aspects and Technological Applications

“…The cationic property of TMC allows its association with polyanionic macromolecules, like heparin, sodium alginate, among others [20,21]. TMC/heparin multilayer thin films carried out from the layer-by-layer assembly [20,22].…”

“…The cationic property of TMC allows its association with polyanionic macromolecules, like heparin, sodium alginate, among others [20,21]. TMC/heparin multilayer thin films carried out from the layer-by-layer assembly [20,22]. Such assembled film prepared with O-methyl TMC, containing 80% DQ showed stronger bactericidal action (64.6% inhibition at 6 h) against E. coli (ATCC 26922) at physiologic pH condition (7.4) [22].…”