Advance in chitosan hydrolysis by non-specific cellulases

Xia, Wenshui; Ping, Liu; Liu, Jing

doi:10.1016/j.biortech.2008.01.011

Cited by 144 publications

(84 citation statements)

References 55 publications

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“…In this study the effect pH was not investigated due to limited variation of pH could be conducted. In addition, previous publications reported that the optimal pH of the chitosan hydrolysis using cellulase enzyme was within the range 4.0 -5.5 [25].…”

Section: Hydrolysis Of Chitosanmentioning

confidence: 95%

Enhanced Enzymatic Hydrolysis of Chitosan by Surfactant Addition

Rokhati

Susanto

Haryani

et al. 2017

Period. Polytech. Chem. Eng.

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Section: Hydrolysis Of Chitosanmentioning

confidence: 95%

Enhanced Enzymatic Hydrolysis of Chitosan by Surfactant Addition

Rokhati

Susanto

Haryani

et al. 2017

Period. Polytech. Chem. Eng.

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“…27,28 First, glycol chitosan (6 g) was dissolved in 400 mL of distilled water overnight. Then 11 mL of acetic acid and cellulase (0.12 g) were added under magnetic stirring.…”

Section: Degradation Of Glycol Chitosanmentioning

confidence: 99%

Fabrication and characterization of nuclear localization signal-conjugated glycol chitosan micelles for improving the nuclear delivery of doxorubicin

Yu¹,

Xie²,

Zheng³

et al. 2012

IJN

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Background: Supramolecular micelles as drug-delivery vehicles are generally unable to enter the nucleus of nondividing cells. In the work reported here, nuclear localization signal (NLS)-modified polymeric micelles were studied with the aim of improving nuclear drug delivery. Methods: In this research, cholesterol-modified glycol chitosan (CHGC) was synthesized. NLSconjugated CHGC (NCHGC) was synthesized and characterized using proton nuclear magnetic resonance spectroscopy, dynamic light scattering, and fluorescence spectroscopy. Doxorubicin (DOX), an anticancer drug with an intracellular site of action in the nucleus, was chosen as a model drug. DOX-loaded micelles were prepared by an emulsion/solvent evaporation method. The cellular uptake of different DOX formulations was analyzed by flow cytometry and confocal laser scanning microscopy. The cytotoxicity of blank micelles, free DOX, and DOX-loaded micelles in vitro was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in HeLa and HepG2 cells. Results: The degree of substitution was 5.9 cholesterol and 3.8 NLS groups per 100 sugar residues of the NCHGC conjugate. The critical aggregation concentration of the NCHGC micelles in aqueous solution was 0.0209 mg/mL. The DOX-loaded NCHGC (DNCHGC) micelles were observed as being almost spherical in shape under transmission electron microscopy, and the size was determined as 248 nm by dynamic light scattering. The DOX-loading content of the DNCHGC micelles was 10.1%. The DOX-loaded micelles showed slow drug-release behavior within 72 hours in vitro. The DNCHGC micelles exhibited greater cellular uptake and higher amounts of DOX in the nuclei of HeLa cells than free DOX and DOX-loaded CHGC (DCHGC) micelles. The half maximal inhibitory concentration (IC 50 ) values of free DOX, DCHGC, and DNCHGC micelles against HepG2 cells were 4.063, 0.591, and 0.171 µg/mL, respectively. Moreover, the IC 50 values of free DOX (3.210 µg/mL) and the DCHGC micelles (1.413 µg/mL) against HeLa cells were nearly 6.96-and 3.07-fold (P , 0.01), respectively, higher than the IC 50 value of the DNCHGC micelles (0.461 µg/mL). Conclusion:The results of this study suggest that novel NCHGC micelles could be a potential carrier for nucleus-targeting delivery.

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“…Chitosan (CH) carries a net positive charge due to -NH 3 + groups and can be easily cross-linked with other anions, oppositely charged drugs and polymers [9]. CH is easily degraded by lysozyme, by non specific cellulases and enzymes secreted by intestinal bacteria [10]. Guar gum has also been examined for use in colonic drug delivery [11].…”

Section: Introductionmentioning

confidence: 99%

Investigations on chitosan-carboxymethyl guar gum complexes interpolymer complexes for colon delivery of fluticasone.

Kumar¹,

Tiwary²,

Kaur³

2010

Int j Drug delivery

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The present study was designed to formulate colon release tablets of fluticasone by employing cross linked chitosan (CH) and carboxymethyl guar gum (CMG) interpolymer complexes (IPC). Matrix tablets were prepared by wet granulation method using IPC as binder and coating agent. The IPC were characterized by Fourier transform infrared spectroscopy (FTIR). The uncoated and coated tablets were tested for their suitability as colon specific drug delivery system by in vitro dissolution studies. The coated tablets were evaluated for their pharmacodynamic performance after oral administration to TNBS induced ulcerative colitic rats. FTIR studies demonstrated that the IPC was formed through an electrostatic interaction between -COO − groups of CMG and -NH 3 + groups of CH. Tablets formulated with 50:50 CH:CMG as binder and coated with the respective ratio of IPC was capable of protecting the drug release in stomach and small intestine and delivering the drug in the colon. Histopathology of the rat colon after oral administration of these IPC film coated tablets revealed significantly greater (p<0.05) reduction in TNBS-induced ulcerative colitis The study confirmed that selective delivery of fluticasone to the colon can be achieved using cross-linked CH and CMG polysaccharides.

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Advance in chitosan hydrolysis by non-specific cellulases

Cited by 144 publications

References 55 publications

Enhanced Enzymatic Hydrolysis of Chitosan by Surfactant Addition

Enhanced Enzymatic Hydrolysis of Chitosan by Surfactant Addition

Fabrication and characterization of nuclear localization signal-conjugated glycol chitosan micelles for improving the nuclear delivery of doxorubicin

Investigations on chitosan-carboxymethyl guar gum complexes interpolymer complexes for colon delivery of fluticasone.

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