Adsorptive stripping voltammetry of nicardipine at a HMDE; determination of trace levels nicardipine in blood and urine

Obendorf, Dagmar; Stubauer, Gottfried

doi:10.1016/0731-7085(95)01554-x

Cited by 13 publications

(6 citation statements)

References 9 publications

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“…Several methods have been reported for determination of nicardipine hydrochloride. These include spectrophotometry [3][4][5] , voltammetry [6][7][8] , high performance liquid chromatography [9][10][11][12] , liquid chromatography mass spectroscopy [13][14][15] and capillary electrophoresis 16 . Most of these methods were used for quantitative determination of nicardipine hydrochloride in biological fluids.…”

Section: Introductionmentioning

confidence: 99%

A Selective High Performance Liquid Chromatographic Method to Follow the Hydrolytic Degradation of Nicardipine Hydrochloride

Ibrahim

Al-Ashban

2009

Journal of Chemistry

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A simple, stability indicating, reverse phase high performance liquid chromatographic method was developed and validated for determination of nicardipine hydrochloride (NC) in the presence of its degradation products. The chromatographic separation was performed on Hypersil, BDS-C18, 30 cm × 3.9 mm id, at ambient temperature with UV-detection at 254 nm. A mixture of 20% (v/v) aqueous 0.01 M sodium acetate∕acetic acid buffer (pH 4.5) and 80% acetonitrile was used as the mobile phase at a flow rate of 1.5 mL min-1, losartan was used as internal standard. The calibration curve is linear over the concentration range 5-40 μg mL-1, with a regression coefficient of 0.9984 and the % recovery was 99.78±0.17. The method was used to investigate the kinetics of alkaline, acids induced degradation, effect of buffer concentration and temperature. The degradation followed first-order kinetics. The rate constant, half-life time, and activation energy were calculated

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Section: Introductionmentioning

confidence: 99%

A Selective High Performance Liquid Chromatographic Method to Follow the Hydrolytic Degradation of Nicardipine Hydrochloride

Ibrahim

Al-Ashban

2009

Journal of Chemistry

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“…4‐Nitrophenyl substituted 1,4‐dihydropyridines contain at least two electroactive sites, an aromatic nitro group that can be reduced in protic media in a four electron step to a hydroxylamine derivative and a dihydropyridine ring that can be oxidized to the corresponding pyridine ring in a two electron step 32.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, αn α was obtained as 1.18, assuming n α =2 31, then α =0.59. In addition, the value of αn α was calculated for the reduction of nifedipine for both MWCNTs/β‐CD/CPE and CPE using the following equation 32: …”

Section: Resultsmentioning

confidence: 99%

β‐Cyclodextrin Incorporated Carbon Nanotube Paste Electrode as Electrochemical Sensor for Nifedipine

Kor

Zarei

2013

Electroanalysis

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In this work a carbon paste electrode modified with multiwalled carbon nanotubes/b-cyclodextrin (MWCNTs/b-CD) was constructed and applied to the determination of nifedipine. The electrochemical behavior of nifedipine at this electrode was investigated using cyclic voltammetry and differential pulse voltammetry. Characterization of the modified electrode was conducted with electrochemical impedance spectroscopy and scanning electron microscopy. After adsorption of nifedipine on the MWCNTs/b-CD paste electrode at 0.0 V for 6 min, a well defined reduction peak was produced in sodium hydroxide of 0.05 M. The calibration curve was linear from 7.0 10 À8 to 1.5 10 À5 M. The detection limit was obtained as 2.5 10 À8 M. The results demonstrated that this electrochemical sensor has excellent sensitivity and selectivity. This sensor was applied for determination of nifedipine in drug dosage and blood serum with excellent recoveries.

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“…Several methods for NC determination in human or laboratory animals plasma have been reported, including gas chromatography (GC) (Watari et al, 1990), GC with electron-capture detection (Higuchi et al, 1975), GCmass spectrometry (Marciniec and Kujawa, 1995), combination of thin-layer chromatography with GC and mass spectrometric detection (Higuchi and Kawamura, 1981) and voltammetry (Obendorf and Stubauer, 1995). However, some of these methods are too complex and cumbersome for the routine analysis of large numbers of samples and involve unconventional, specialized and expansive equipment (Higuchi and Kawamura, 1981) and others are not sensitive enough for accurate quantification of low therapeutic concentrations of NC (Obendorf and Stubauer, 1995), which limits their application.…”

Section: Introductionmentioning

confidence: 99%

“…However, some of these methods are too complex and cumbersome for the routine analysis of large numbers of samples and involve unconventional, specialized and expansive equipment (Higuchi and Kawamura, 1981) and others are not sensitive enough for accurate quantification of low therapeutic concentrations of NC (Obendorf and Stubauer, 1995), which limits their application. In addition, some of these methods (eg GC techniques) are relatively non-specific because require NC oxidation prior to the analysis.…”

Section: Introductionmentioning

confidence: 99%

A simple method for nicardipine hydrochloride quantification in plasma using solid‐phase extraction and reversed‐phase high‐performance liquid chromatography

Fernandes

Veiga

2003

Biomedical Chromatography

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A simple and sensitive reversed-phase liquid chromatography method was developed and validated for the determination of nicardipine hydrochloride (NC) in rabbit plasma. Nicardipine hydrochloride and nimodipine, used as internal standard, were initially extracted from plasma by a rapid solid-phase extraction using C 18 cartridges. After extraction, nicardipine hydrochloride was separated by HPLC on a C 18 column and quantified by ultraviolet detection at 254 nm. A mixture of acetonitrile-0.02 M sodium phosphate buffer-methanol (45:40:15) with 0.2% of triethylamine of pH of 6.1 was used as mobile phase. The mean (AESD) extraction efficiency of NC was 77.56 AE 5.4, 84.23 AE 4.32 and 83.94 AE 3.87% for drug concentrations of 5, 25 and 100 ng/mL, respectively. The method proved to be linear in the range of 5-100 ng/mL with a regression coefficient of 0.9993. The relative standard deviations of intra-and inter-day analysis for NC in plasma were 3.26-6.52% (n = 5) and 4.71-9.38% (n = 5), respectively. The differences of the mean value measured from the concentration prepared, expressed in percentages (bias percentage), were only À 5.2, 0.4 and 0.8% at NC 5, 25 and 50 ng/mL, which confirmed the accuracy of the method. The analytical technique was used to determine NC plasma concentration after drug oral administration to rabbits. The results inferred that NC is rapidly absorbed in rabbits and has a short half-life (t 1/2 = 1.34 h).

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Adsorptive stripping voltammetry of nicardipine at a HMDE; determination of trace levels nicardipine in blood and urine

Cited by 13 publications

References 9 publications

A Selective High Performance Liquid Chromatographic Method to Follow the Hydrolytic Degradation of Nicardipine Hydrochloride

A Selective High Performance Liquid Chromatographic Method to Follow the Hydrolytic Degradation of Nicardipine Hydrochloride

β‐Cyclodextrin Incorporated Carbon Nanotube Paste Electrode as Electrochemical Sensor for Nifedipine

A simple method for nicardipine hydrochloride quantification in plasma using solid‐phase extraction and reversed‐phase high‐performance liquid chromatography

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