Adsorption Process of Molecularly Imprinted Silica for Extraction of Lactose From Milk

Abstract

-In Brazil, about 25-30% of the population has some degree of intolerance to lactose, a disorder associated with the inability of the body to digest lactose due to a disability or absence of the enzyme lactase. The goal of this study was to evaluate the performance of adsorption of lactose from fresh milk using a fixed bed column of molecularly imprinted polymer (MIP). The polymeric material was characterized using Scanning electron microscopy (SEM) analysis, thermal analysis (e.g., differential scanning calorimetric (DSC) and thermogravimetric analysis (TGA), Fourier Transform Infrared Spectroscopy (FTIR), and the method of Braunauer, Emmet and Teller (BET). The adsorption column dynamics and performance were studied by the breakthrough curves using a 2 4-1 fractional factorial design. The chemical and structural characterization of the pure matrix and imprinted polymers confirmed the molecularly imprinted polymer (MIP) imprinted with lactose. The highest capacity was 62.21 mgg -1 , obtained at 307.1 K and a flow rate of 12.5 mL.min -1 , with central point conditions, 320.1 K and 9 mL.min -1 , with an average value of 50.9 mg.g -1 . The results indicate that the molecularly imprinted polymer is efficient.

Effects of Processing on Structure and Thermal Properties of Powdered Preterm Infant Formula

“…The second endothermic transition of F samples occurred between 114 and 160 °C (Figure B, peak b). The previous study reported that dehydration endotherm may occur in lactose DSC analysis at around 135 °C (Balieiro et al., ; Kaialy, Larhrib, Martin, & Nokhodchi, ; Yuan, Hourigan, Sleigh, & Steele, ), so peak g represented dehydration endotherm of lactose. However, T on and T p values were obviously increased after homogenization ( T on from 114.9 to 128.8 °C, T p from 124.5 to 140.7 °C), and then decreased slightly after following processing but still higher than that of MIF (114.9, 128.8, 125.0, 120.2, and 121.8 °C for MIF, HIF, PIF, EIF, and SIF).…”

Modeling of milk lactose removal by column adsorption using artificial neural networks: MLP and RBF

“…The preparation of the adsorbent and the analytical determination of the lactose were performed as described by Balieiro et al [8]. The adsorbent was prepared using the sol-gel process and tetraethoxysilane (TEOS) as raw material.…”

“…The mobile phase consisted of acetonitrile and ultrapure water (60:40) at a flow rate of 1.5 mL min -1 , with an analysis time of 10 min. For the determination of lactose from milk samples: 30 µL samples were diluted in a 2 mL volumetric flask in ultrapure water and then the samples were filtered through a 0.45 Millipore membrane and subsequently injected into the equipment [8].…”

“…The main advantage that MIPs have over the conventional materials used in extraction in the solid phase, is their molecular specificity [7]. Balieiro et al [8] and Oliveira et al [9] studied the adsorption process of molecularly imprinted silica in a fixed-bed column with continuous flow, for the extraction of lactose and cholesterol from milk, respectively.…”

“…Some studies have used phenomenological models to describe the adsorption mechanism [8][9][10][11][12][13][14][15]. In addition to the phenomenological models, the literature abounds with studies that use empirical models developed using artificial neural network (ANN) techniques, to obtain a model that best describes various adsorption processes [9,11,[16][17][18][19][20][21][22].…”